Isolation, Molecular Characterization and Determination of Antagonistic Properties of Alkalitolerant Streptomyces Members from Van Lake-Çarpanak Island Soil

Abstract

Çarpanak Island is one of the islands in Lake Van, the easternmost and largest lake in Türkiye. In this study, soil samples taken from three different locations of Çarpanak island, Lake Van, were inoculated into 4 different selective isolation media using the dilution plate technique, and 42 actinomycete isolates were stocked. PCR amplification of the 16S rRNA gene of 13 isolates selected according to their morphological differences was performed with 27F and 1525R primers. Phylogenetic trees were constructed with the neighbor-joining algorithm in the MEGA 7.0 software. According to 16S rRNA gene sequence analysis, it was determined that 13 isolates belonged to the genus Streptomyces. The antimicrobial activities of 13 isolates against 8 pathogens and the ability of these isolates to produce lipase, amylase, protease and pectinase were determined. Except for CA43 and CA62 isolates, other Streptomyces isolates have the ability to produce lipase. Other Streptomyces isolates except CA59 and CA94 can synthesize amylase. In addition, 3 of the 13 Streptomyces isolates, CA40, CA61 and CA94, do not have the ability to synthesize protease. No isolate is capable of producing pectinase enzyme. As a result of this study, it was observed that most of the Streptomyces isolates had higher lipase enzyme production abilities than other enzyme groups.

Keywords

• Alkalitolerant Streptomyces
• Lake Van
• Çarpanak Island
• 16S rRNA gene
• Antimicrobial activity
• Enzyme production

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