The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization

Gamze Uçak; Ali Doğan Ömür; Serkan Ali Akarsu; Esat Mahmut Kocaman; Ahmet Karahanli; Ahmet Yörü; Belkıs Kamer

doi:10.33714/masteb.1736442

The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization

Abstract

Hesperidin is an important flavonoid compound found at high levels in citrus fruits. In addition to its protective effects on vital organs, numerous studies have highlighted its beneficial impact on the reproductive system. Date palm pollen (DPP) is the male reproductive cell of the date palm flower, known for its successful results in the treatment of infertility. The present study aimed to comparatively investigate the effects of hesperidin and date pollen on fertilization and sperm membrane integrity in rainbow trout sperm. Four male rainbow trout were used in the study. Sperm was collected from the trout by abdominal massage method and divided into 5 equal groups: 0.024 DPP, 0.5 DPP, 0.009 hesperidin, normal diluent and native sperm. Each group was diluted with the previously prepared diluent. Sperm was collected from female fish by the abdominal massage method. Egg fluid was obtained and diluted sperm was added. They were left to incubate in the incubator, and the total number of fertilized eggs was determined after 28-30 days. Smear samples were taken from the groups with eosin-nigrosin staining and evaluated for membrane integrity. As a result, fertilization was observed only in the groups reconstituted with normal diluent and hesperidin-added groups. When sperm membrane integrity was evaluated, it was found that the highest membrane damage was observed in the two groups to which date pollen was added. Date palm pollen-added diluent caused an increase in sperm membrane damage in rainbow trout sperm. The addition of hesperidin to the rainbow trout sperm diluent caused an increase in fertilization potential and a positive effect on the preservation of seminal membrane integrity.

Keywords

Ethical Statement

Approval for the study was obtained from Atatürk University Animal Experiments Local Ethics Committee (Protocol no: 2025/02–38).

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Details

Primary Language

English

Subjects

Fish Biology , Fish Breeding , Pisciculture , Fisheries Management , Aquaculture

Journal Section

Research Article

Authors

Gamze Uçak ^*
0009-0006-3532-973X
Türkiye

Ali Doğan Ömür
0000-0002-2976-4368
Türkiye

Serkan Ali Akarsu
0000-0003-4450-6540
Türkiye

Esat Mahmut Kocaman
0000-0002-6426-7260
Türkiye

Ahmet Karahanli
0009-0002-8321-2836
Türkiye

Ahmet Yörü
0000-0002-3232-9105
Türkiye

Belkıs Kamer
0000-0002-9291-5480
Türkiye

Publication Date

September 30, 2025

Submission Date

July 7, 2025

Acceptance Date

September 25, 2025

Published in Issue

Year 2025 Volume: 14 Number: 3

DOI

https://doi.org/10.33714/masteb.1736442

IZ

https://izlik.org/JA96ZZ65HA

Cite

RIS / Bibtex

APA

Uçak, G., Ömür, A. D., Akarsu, S. A., Kocaman, E. M., Karahanli, A., Yörü, A., & Kamer, B. (2025). The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization. Marine Science and Technology Bulletin, 14(3), 149-154. https://doi.org/10.33714/masteb.1736442

AMA

1.Uçak G, Ömür AD, Akarsu SA, et al. The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization. Mar. Sci. Tech. Bull. 2025;14(3):149-154. doi:10.33714/masteb.1736442

Chicago

Uçak, Gamze, Ali Doğan Ömür, Serkan Ali Akarsu, et al. 2025. “The Comparative Effect of Hesperidin and Date Pollen Added to Rainbow Trout (Oncorhynchus Mykiss) Sperm Diluent on Sperm Membrane Integrity and Fertilization”. Marine Science and Technology Bulletin 14 (3): 149-54. https://doi.org/10.33714/masteb.1736442.

EndNote

Uçak G, Ömür AD, Akarsu SA, Kocaman EM, Karahanli A, Yörü A, Kamer B (September 1, 2025) The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization. Marine Science and Technology Bulletin 14 3 149–154.

IEEE

[1]G. Uçak et al., “The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization”, Mar. Sci. Tech. Bull., vol. 14, no. 3, pp. 149–154, Sept. 2025, doi: 10.33714/masteb.1736442.

ISNAD

Uçak, Gamze - Ömür, Ali Doğan - Akarsu, Serkan Ali - Kocaman, Esat Mahmut - Karahanli, Ahmet - Yörü, Ahmet - Kamer, Belkıs. “The Comparative Effect of Hesperidin and Date Pollen Added to Rainbow Trout (Oncorhynchus Mykiss) Sperm Diluent on Sperm Membrane Integrity and Fertilization”. Marine Science and Technology Bulletin 14/3 (September 1, 2025): 149-154. https://doi.org/10.33714/masteb.1736442.

JAMA

1.Uçak G, Ömür AD, Akarsu SA, Kocaman EM, Karahanli A, Yörü A, Kamer B. The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization. Mar. Sci. Tech. Bull. 2025;14:149–154.

MLA

Uçak, Gamze, et al. “The Comparative Effect of Hesperidin and Date Pollen Added to Rainbow Trout (Oncorhynchus Mykiss) Sperm Diluent on Sperm Membrane Integrity and Fertilization”. Marine Science and Technology Bulletin, vol. 14, no. 3, Sept. 2025, pp. 149-54, doi:10.33714/masteb.1736442.

Vancouver

1.Gamze Uçak, Ali Doğan Ömür, Serkan Ali Akarsu, Esat Mahmut Kocaman, Ahmet Karahanli, Ahmet Yörü, Belkıs Kamer. The comparative effect of hesperidin and date pollen added to rainbow trout (Oncorhynchus mykiss) sperm diluent on sperm membrane integrity and fertilization. Mar. Sci. Tech. Bull. 2025 Sep. 1;14(3):149-54. doi:10.33714/masteb.1736442