Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus)

Year 2018, , 1 - 3, 30.06.2018

Azadeh Yektaseresht , Zahra Hemati Amin Gholamhosseini

https://doi.org/10.33714/masteb.375727

Abstract

The immunoglobulins (Igs) were isolated and purified respectively from serum
of Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser
stellatus) by using 50% saturated ammonium sulfate precipitation. Analysis of the
purified Ig of the two species of fishes on sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE) under reducing conditions revealed two bands,
heavy chains and light chains respectively. The molecular weight of the heavy
chains of two fishes was about 70 kD and the molecular weight of the light chains
was 27kD for Siberian sturgeon and 27.5 kD for Stellate sturgeon. The results
suggested that the isolated and purified serum Immunoglobuline in two species of
sturgeons, Siberian sturgeon (A. baerii) and Stellate sturgeon (A. stellatus) can
be used to produce polyclonal and monoclonal antibodies against sturgeon Ig for
developing diagnostic tests against a wide variety of pathogens.

Keywords

Immunoglobulins, serum, Acipenser baerii, Acipenser stellatus

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