The Effects of EDTA on Lead Accumulation in Tissues of Clarias gariepinus

Abstract

This study was intended to determine the lead accumulation levels in liver, gill, kidney, brain, and muscle tissues of Clarias gariepinus (African sharp tooth catfish) under the sole effect of lead (1.0 and 2.0 ppm) and combined with EDTA (Ethylene diamine tetraacetic acid) (2.0 and 4.0 ppm) for 7, 15, and 30 days. Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine tissue lead levels, and the SPSS package program was used for statistical evaluation of the experimental data. No mortality was observed in fish over the time periods of the experiments, and concentrations were determined with lead only and with lead together with EDTA. At the end of the experimental periods, the presence of lead increased the metal accumulation in the tissues and organs examined when compared to the control, and, in terms of accumulation, a relationship was determined between the tissues in the order of gill > kidney >liver > brain > muscle. The effect of lead together with EDTA was reduced lead accumulation in tissues and organs when compared to the effect of lead only.

Keywords

• Clarias gariepinus
• Lead
• EDTA
• Interaction
• Tissue
• Accumulation

References

1. Abdullah, S., Javed, M. & Javid, A. (2007). Studies on acute toxicity of metals to the fish (Labeo rohita). International Journal of Agriculture and Biology, 9(2): 333-337.
2. Alkahemal-Balawi, H. F., Ahmad, Z., Al-Akel, A. S., Al-Misned, F., Suliman, El. A. M. & Al-Ghanim, K. A. (2011). Toxicity bioassay of lead acetate and effects of its sublethal exposure on growth, haematological parameters and reproduction in Clarias gariepinus. African Journal of Biotech, 10(53): 11039-11047. https://doi.org/10.5897/AJB11.1463
3. Al-Balawi, H. F. A., Al-Akel, A. S., Al-Misned, F., El Amin, M. S., Al-Ghanim, K. A., Mahboob, S. & Ahmad, Z. (2013). Effects of sub-lethal exposure of lead acetate on histopathology of gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus. Brazilian Archives of Biology and Technology, 56(2): 293-302. https://doi.org/10.1590/S1516-89132013000200015.
4. Alves, L. C. & Wood, C. M. (2006). The chronic effects of dietary lead in freshwater juvenile rainbow trout (Oncorhynchus mykiss) fed elevated calcium diets. Aquatic Toxicology, 78: 217–232. https://doi.org/10.1016/j.aquatox.2006.03.005
5. Banaee, M., Haghi, B. N. & Zoheiri, F. (2013). LC50 and bioaccumulation of lead nitrate Pb(NO3)2 in goldfish (Carassius auratus). International Journal of Aquatic Biology, 1(5): 233-239. https://doi.org/10.22034/ ijab.v1i5.153
6. Bawa-Allah, K. A. & Saliu, J. K. (2015). Acute toxicity and bioaccumulation patterns of lead and zinc in juveniles of Clarias gariepinus. Journal of Environmental Science Toxicology and Food Technology (IOSR-JESTFT), 9(1): 42-47. https://doi.org/10.9790/2402-09124247
7. Canlı, M. & Kargın, F. (1995). A comparative study on heavy metal (Cd,Cr, Pb and Ni) accumulation in the tissue of the carp Cyprinus carpio and the nile fish Tilapia nilotica. Turkish Journal of Veterinary and Animal Sciences, 19: 165–171.
8. Cengiz, E. İ. (2006). Gill and kidney histopathology in the freshwater fish Cyprinus carpio after acute exposure to deltamethrin. Environmental Toxicology and Pharmacology, 22: 200–204. https://doi.org/10.1016/j.etap.2006.03.006

9. Cicik, B., Ay, Ö. & Karayakar, F. (2004). Effects of lead and cadmium interaction on the metal accumulation in tissue and organs of Nile tilapia Oreochromis niloticus. Bulletin of Environmental Contamination and Toxicology, 1(72): 141-148. https://doi.org/10.1007/s00128-003-0252-5
10. Clearwater, S. J., Farag, A. M. & Meyer, J. S. (2002). Review: Bioavailability and toxicity of dietborne copper and zinc to fish. Comparative Biochemistry and Physiology - Part C, 132: 269–313. https://doi.org/10.1016/S1532-0456(02)00078-9
11. Çiftçi, N., Cicik, B., Erdem, C. & Ay, Ö. (2008). Effects of lead concentrations on sera parameters and hematocrit levels in Anguilla anguilla (Linnaeus, 1758). Journal of Fisheries Sciences, 2(4): 616-622. https://doi.org/10.3153/jfscom.2008025
12. Eisler, R. (2000). Handbook of chemical risk assessment health hazards to humans, plants and animals: Vol 1, United States of America, 844p.
13. Figueiredo-Fernandes, A., Fontanhas-Fernandes, A., Rocha, E. & Reis-Henriques, M. A. (2006). The effect of paraquat on hepatic EROD activity, liver and gonadal histology in males and females of Nile tilapia, Oreochromis niloticus, exposed at different temperatures. Archives of Environmental Contamination and Toxicology, 51: 626–632. https://doi.org/10.1007/s00244-005-0208-3
14. Gül, A., Yılmaz, M. & Işılak, Z. (2009). Acute toxicity of zinc sulphate (ZnSO4.H2O) to guppies (Poecilia reticulata P.1859). Gazi University Journal of Science, 22(2): 59-65.
15. James, R., Sampath, K. & Selvamani, P. (1998). Effect of EDTA on reduction of copper toxicity in Oreochromis mossambicus (Peters). Bulletin of Environmental Contamination and Toxicology, 60: 487-493. https://doi.org/10.1007/s001289900651
16. Jezierska, B. & Witeska, M. (2006). The metal uptake and accumulation in fish living in polluted waters. Soil and Water Pollution Monitoring, Protection and Remediation, 3(23): 107-113. https://doi.org/10.1007/978-1-4020-4728-2_6
17. Karaman, Z. & Dörücü, M. (2017). Balıklarda bağışıklık sistemi organları ve histolojisi. International Journal of Pure and Applied Sciences, 3(1): 65-74.
18. Karataş, S. & Kalay, M. (2002). Tilapia zilli’nin solungaç, karaciğer, böbrek ve beyin dokularında kurşun birikimi. Turkish Journal of Veterinary and Animal Sciences, 26: 471-477.
19. Katti, S. R. & Sathyanesan, A. G. (1986). Lead nitrate induced changes in the brain constituents of the freshwater fish Clarias batrachus (L). Neurotoxicology, 7(3): 47–52.
20. Kedziorek, M. A. M. & Bourg, A. C. M. (2000). Solubilization of lead and cadmium during the percolation of EDTA through a soil polluted by smelting activities. Journal of Contaminant Hydrology, 40: 381–392. https://doi.org/10.1016/S0169-7722(99)00056-X
21. Khidr, B. M., Mekkawy, I. A. A., Harabawy, A. S. A. & Ohaida, A. S. M. I. (2012). Effect of lead nitrate on the liver of the cichlid fish (Oreochromis niloticus): A light microscope study. Pakistan Journal of Biological Sciences, 15(18): 854-862. https://doi.org/10.3923/pjbs.2012.854.862
22. Kusemiju, V., Patience, A. & Oluwatoyin, A. J. (2012). Accumulation of lead in the tissues of freshwater catfish Clarias gariepinus exposed to static nominal concentrations of lead nitrate. Agriculture and Biology Journal of North America, 3(12): 510-515. https://doi.org/10.5251/abjna.2012.3.12.510.515
23. Łuszczek-Trojnar, E., Sionkowski, J., Drazg-Kozak, E. & Popek, W. (2016). Copper and lead accumulation in common carp females during long-term dietary exposure to these metals in pond conditions. Aquaculture Research, 47: 2334–2348. https://doi.org/10.1111/are.12689
24. Martinez, C. B. R., Nagae, M. Y., Zaia, C. T. B. V. & Zaia, D. A. M. (2004). Acute morphological and physiological effects of lead in the neotropical fish Prochilodus lineatus. Brazilian Journal of Biology, 64(4): 797-807. https://doi.org/10.1590/S1519-69842004000500009
25. Mazon, A. F., Monteiro, E. A. S., Pinheiro, G. H. D. & Fernandes, M. N. (2002). Hematological and physiological changes induced by short-term exposure to copper in the freshwater fish, Prochilodus Scrofa. Brazilian Journal of Biology, 62(4A): 621-631. https://doi.org/10.1590/S1519-69842002000400010.
26. Mohanambal, R. & Puvaneswari, S. (2013). Bioaccumulatıon of lead in various tissues of the freshwater fish Catla catla (Hamilton, 1822). International Journal of Development Research, 3(8): 54-60.
27. Morgan, J. D., Sakamoto, T., Grau, E. G. & Iwama, G. K. (1997). Physiological and respiratory responses of the Mozambique tilapia (Oreochromis mossambicus) to salinity acclimation. Comparative Biochemistry and Physiology, 117A(3): 391–398. https://doi.org/ 10.1016/S0300-9629(96)00261-7
28. Muramoto, S. (1980). Effects of complexans (EDTA, NTA and DTPA) on the exposure to high concentrations of cadmium, copper, zinc and lead. Bulletin of Environmental Contamination and Toxicology, 25: 941-946. https://doi.org/10.1007/BF01985635
29. Muramoto, S. (1983). Elimination of copper from Cu-contaminated fish by long term exposure to EDTA and freshwater. Journal of Environmental Science and Health, 18(3): 455-461. https://doi.org/10.1080/10934528309375113
30. Ogbuagu, D. H., Adebayo, E. T., Ayoade, A. A., Ugwu, O. B. & Mba, D. O. (2015). Lead accumulation in and its haematological effects on African catfish Clarias gariepinus. African Journal of Aquatic Science, 40(2): 201–204. https://doi.org/10.2989/16085914.2015.1028325
31. Poleksic, V. & Mitrovic-Tutundzic, V. (1994). Fish gills as a monitor of sublethal and chronic effects of pollution (pp. 339-352). In: Mulls, R, Llyod (Eds) on freshwater fish. Oxford, London, UK: Fishing News books.
32. Ribeiro, A. M., Risso, W. E., Fernandes, M. N. & Martinez, C. B. R. (2014). Lead accumulation and its effects on the branchial physiology of Prochilodus lineatus. Fish Physiology and Biochemistry, 40: 645–657. https://doi.org/10.1007/s10695-013-9873-8
33. Shalaby, A. M. E. (2007). Effect of EDTA on toxicity reduction of cadmium in relation to growth some haematological and biochemical profiles of Nile tilapia (Oreochromis niloticus). Journal of Fisheries and Aquatic Science, 2(2): 100-109.
34. Shukla, V., Dhankhar, M., Prakash, J. & Sastry, K.V. (2007). Bioaccumulation of Zn, Cu and Cd in Channa punctatus. Journal of Environmental Biology, 28(2): 395-397. Soengas, J. L. & Aldegunde, M. (2002). Review. Energy metabolism of fish brain. Comparative Biochemistry and Physiology Part B, 131: 271–296. https://doi.org/10.1016/S1096-4959(02)00022-2
35. Tao, S., Li, H., Liu, C. & Lam, K. C. (2000). Fish uptake of inorganic and mucus complexes of lead. Ecotoxicology and Environmental Safety, 46: 174-180. https://doi.org/10.1006/eesa.1999.1902
36. Van Dyk, J. C., Pieterse, G. M. & Van Vuren, J. H. J. (2007). Histological changes in the liver of Oreochromis mossambicus (Cichlidae) after exposure to cadmium and zinc. Ecotoxicology and Environmental Safety, 66: 432–440. https://doi.org/10.1016/j.ecoenv.2005.10.012
37. Vosylienė, M. Z. (1999). The effect of heavy metals on haematological indices of fish (survey). Acta Zoologica Lituanica. Hydrobiologia, 9(2): 76-82. https://doi.org/10.1080/13921657.1999.10512290