In the present study, effects of different diets on the fatty acid composition of hatched seabass (Dicentrarchus labrax) at after fertilization of the egg (E), and yolk sac (Y) stages have been investigated. In the study, the fish were fed with Artemia (A), Artemia-microparticulate mix (AMD) and microparticulate diet (M). According to the results of the study, the levels of total PUFAs in the total lipid (TL) were significantly higher in eggs (E) (P<0.05) compared to the other groups. 14:1, 15:1, 17:1 and 22:1n 9 and also aracidonic acid and docosaksaneoic acid could not be detected in Artemia but detectable in E and Y groups. During the development stages, polyunsaturated fatty acids (PUFA), arachidonic (20:4n6 ArA), eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) were decreased significantly (P<0.05) till the AMD stage. ArA, EPA and DHA were used as energy sources by Dicentrarchus labrax larvae. Total n-3 content showed the highest levels at the AMD stage, which was followed by the M, E, Y and A stages, respectively. EPA/DHA ratio was the highest in the AMD group (P>0.05). In the study, seabass fatty acid composition at different growth stages was affected by diet composition. After feeding stage especially in the AMD and M groups, essential fatty acids were provided from diets.