Year 2018, Volume 7 , Issue 1, Pages 1 - 3 2018-06-30

Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus)

Azadeh Yektaseresht [1] , Zahra Hemati [2] , Amin Gholamhosseini [3]

The immunoglobulins (Igs) were isolated and purified respectively from serum of Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus) by using 50% saturated ammonium sulfate precipitation. Analysis of the purified Ig of the two species of fishes on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed two bands, heavy chains and light chains respectively. The molecular weight of the heavy chains of two fishes was about 70 kD and the molecular weight of the light chains was 27kD for Siberian sturgeon and 27.5 kD for Stellate sturgeon. The results suggested that the isolated and purified serum Immunoglobuline in two species of sturgeons, Siberian sturgeon (A. baerii) and Stellate sturgeon (A. stellatus) can be used to produce polyclonal and monoclonal antibodies against sturgeon Ig for developing diagnostic tests against a wide variety of pathogens.

Immunoglobulins, serum, Acipenser baerii, Acipenser stellatus
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Primary Language en
Subjects Marine and Freshwater Biology
Journal Section Research Article

Orcid: 0000-0003-1479-1723
Author: Azadeh Yektaseresht (Primary Author)
Institution: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Country: Iran

Orcid: 0000-0003-1456-1824
Author: Zahra Hemati
Institution: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Country: Iran

Orcid: 0000-0003-1645-1569
Author: Amin Gholamhosseini
Institution: Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Country: Iran


Application Date : January 6, 2018
Acceptance Date : March 27, 2018
Publication Date : June 30, 2018

APA Yektaseresht, A , Hemati, Z , Gholamhosseini, A . (2018). Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus). Marine Science and Technology Bulletin , 7 (1) , 1-3 . DOI: 10.33714/masteb.375727