Investigation of the Effects of Whey Powder on Haematococcus pluvialis Cell Growth Kinetics

Year 2022, Volume: 11 Issue: 3, 343 - 351, 30.09.2022

Büşra Aydoğdu Özden Fakıoğlu

https://doi.org/10.33714/masteb.1134451

Abstract

This study was carried out to examine the effect on the growth of Haematococcus pluvialis using low-cost whey powder (WP). The H. pluvialis used in this study were from Çukurova University, Faculty of Fisheries, and 70% demineralized WP from Cici Dairy Industry Trade Inc. The experiment was conducted under laboratory conditions with 3 replications. During this experiment, cell numbers and biomass were analyzed every day. In addition, the specific growth rate of H. pluvialis was calculated according to the Monod Equivalence. The mean values of the cell number following the WP application were calculated according to the groups (C, W5, W10 and W15) as 763.34±419.62 cells/ml, 951.60±388.20 cells/ml, 1105.27±380.35 cells/ml and 978.63±411.07 cells/ml, respectively. The mean biomass value has been found the lowest in the control group (0.84±0.36 g/l), and the highest value in the W15 group (1.26±0.55 gl^-1). The mean specific growth rate was determined as 0.52±0.09 day^-1 in the control group, 0.56±0.1 day^-1 in the W5 group, 0.56±0.14 day^-1 in the W10 group, and as 0.61±0,09 day^-1 in the W15 group. In accordance with the data obtained, both the biomass and the specific growth rate of H. pluvialis were observed to increase in the W15 group. The use of WP as a nutrient medium is recommended due to its low-cost as well as increasing biomass.

Keywords

Whey powder, Biomass, Specific growth rate, Haematococcus pluvialis

Supporting Institution

Çukurova University

Project Number

FYL-2021-8908

Thanks

H. pluvialis strain was obtained from Çukurova University (Assoc. Prof. Dr. Leyla Hızarcı USLU), This research was supported by the Coordinatorship of Scientific Research Projects (grant no: FYL-2021-8908) and this manuscript was produced from the master thesis of the first author under the supervision of the second author.

References

• Abreu, A. P., Fernandes, B., Vicente, A. A., Teixeira, J., & Dragone, G. (2012). Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source. Bioresource Technology, 118, 61-66. https://doi.org/10.1016/j.biortech.2012.05.055
• Akın, O. (2005). Haematococcus pluvialis mikroalginden astaksantin ekstraksiyonu. [MSc. Thesis. Ege University].
• Anonymous, (2003). Water quality-guidance standard for the routine analysis of phytoplankton abundance and composition using inverted microscopy (Utermöhl Technique). (CEN TC 230/WG 2/TG 3/N73), 37 p.
• Anonymous. (2022). Media preparation recipe template. Retrieved on March 6, 2022, from https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf
• Aslanbay Güler, B., & İmamoğlu, E. (2021). Mikroalgal üretimlerde kinetik modelleme [Kinetic modelling of microalgae productions]. Journal of Limnology and Freshwater Fisheries Research, 7(2), 176-183. https://doi.org/10.17216/limnofish.787055
• Bischoff, H. W., & Bold, H. C. (1963). Phycological studies IV: Some algae from Enchanted Rock and related algal species. University of Texas.
• Bold, H. C. (1949). The morphology of Chlamydomonas chlamydogama, sp. nov. Bulletin of the Torrey Botanical Club, 76(2), 101-108. https://doi.org/10.2307/2482218
• Bougaran, G., Bernard, O., & Sciandra, A. (2010). Modeling continuous cultures of microalgae colimited by nitrogen and phosphorus. Journal of Theoretical Biology, 265(3), 443-454. https://doi.org/10.1016/j.jtbi.2010.04.018
• Çokcan, C. (2015). Haematococcus pluvialis alginden astaksantin üretimi [MSc. Thesis. Yıldız Teknik University].
• Eriştürk, S. (2005). Haematococcus pluvialis’in fotobiyoreaktörlerde üretimine etki eden parametrelerin optimizasyonu ve similasyonu [MSc. Thesis. Ege University].
• Girard, J. M., Roy, M. L., Hafsa, M. B., Gagnon, J., Faucheux, N., Heitz, M., Tremblay, R., & Deschênes, J. S. (2014). Mixotrophic cultivation of green microalgae Scenedesmus obliquus on cheese whey permeate for biodiesel production. Algal Research, 5, 241-248. https://doi.org/10.1016/j.algal.2014.03.002
• Göksan, T. (2003). Haematococcus pluvialis flotow’un (Chlorophyceae) laboratuvar ve dış ortam koşullarında kültürü sırsında pigment kompozisyonu ve fotokimyasal parametrelerinde meydana gelen değişimler [Ph.D. Thesis. Ege University].
• İmamoğlu, E. (2005). Haematococcus pluvialis üretiminde vejatatif fazda ve kistik fazda optimum ortam, sıcaklık ve ışık koşullarının tespiti [MSc. Thesis. Ege University].
• Kalmaoğlu, G. (2020). Effects of green light spectrum on growth and gene expression profiles of green algae Haematococcus pluvialis. [MSc. Thesis. Bolu Abant İzzet Baysal University].
• Kang, C. D., Lee, J. S., Park, T. H., & Sim, S. J. (2005). Comparison of hetetrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis. Applied Microbiology and Biotechnology, 68(2), 237-241. https://doi.org/10.1007/s00253-005-1889-2
• Klausmeier, C. A., Litchman, E., & Levin, S. A. (2004). Phytoplankton growth and stoichiometry under multiple nutrient limitation. Limnology and Oceanography, 49(4 part2), 1463-1470. https://doi.org/10.4319/lo.2004.49.4_part_2.1463
• Köksal, M. (2008). Farklı ortam koşullarının (işık, sıcaklık, besin eksikiği ve havalandırma) Haematococcus pluvialis flotow’da büyüme ve astaksantin miktarına etkisi [MSc. Thesis. Çukurova University].
• Sasa, A., Şentürk, F., Üstündağ, Y., & Erem, F. (2020). Alglerin Gıda veya Gıda Bileşeni Olarak Kullanımı ve Sağlık Üzerine Etkileri [Use of algae as foods or food ingredients and their effects on health]. International Journal of Engineering, Design and Technology, 2(2), 97-110.
• Tsolcha, O. N., Tekerlekopoulou, A. G., Akratos, C. S., Bellou, S., Aggelis, G., Katsiapi, M., Moustaka-Gouni, M., & Vayenas, D. V. (2015). Treatment of second cheese whey effluents using a Choricystis‐based system with simultaneous lipid production. Journal of Chemical Technology & Biotechnology, 91(8), 2349-2359. https://doi.org/10.1002/jctb.4829
• Utermöhl, H. (1958). Zur Vervollkommnung der quantitativen Phytoplankton-Methodik [Methods of collecting plankton for various purposes are discussed]. SIL Communications 1953-1996: Internationale Vereinigung für Theoretische und Angewandte Limnologie: Mitteilungen, 9(1), 1-38. https://doi.org/10.1080/05384680.1958.11904091
• Wang, S. K., Wang, X., Miao, J., & Tian, Y. T. (2018). Tofu whey wastewater is a promising basal medium for microalgae culture. Bioresource Technology, 253, 79-84. https://doi.org/10.1016/j.biortech.2018.01.012
• Yıldırım, Ç., & Güzeler, N. (2013). Peyniraltı suyu ve yayıkaltının toz olarak değerlendirilmesi [Evaluation of whey and buttermilk as powder]. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 28(2), 11-20.
• Giritli Yılmaz, S. (2019). Haematococcus pluvialis flotow (Chlorophyceae) mikroalginin fototrofik kültüründe farklı renkte led ışıkların etkisi [MSc. Thesis. Çanakkale Onsekiz Mart University].