Yonca Kuru Otuna (Medicago sativa) Atık Kahve Ekstraktı İlavesinin Antimetanojenik Etkisinin İn Vitro Gaz Üretim Metoduyla Belirlenmesi

Yıl 2022, Cilt: 2 Sayı: 2, 77 - 82, 30.09.2022

Bilal Selçuk , Tuğba Bakır , Ali Kaya , Atilla Başer

Öz

Bu çalışmada, çiçeklenme döneminde hasat edilmiş olan yonca kuru otuna (Medicago S.) farklı dozlarda atık kahve ekstraktı ilavesinin; yonca kuru otunun fermantasyon parametreleri; pH değerleri, amonyak azotu (NH3-N) üretim miktarları, metabolize edilebilir enerji ve in vitro organik madde sindirimi üzerindeki etkileri belirlenmiştir. Atık kahve ekstraktı ilavesi yonca kuru otunun in vitro gaz üretimlerini ve metan üretim değerlerini önemli düzeyde etkilemiştir (P<0.001). In vitro gaz üretim metodu ile belirlenen 24 saatlik gaz üretim miktarları 44.76 ile 62.52 ml 200mg-1 kuru madde (KM) arasında bulunmuştur. Yonca kuru otunun gaz üretim değerleri atık kahve ekstraktı ilavesi ile önemli derece artmış olup en yüksek değer 1.2 ml ilaveli grupta bulunmuştur. Fermantasyon sonrası % CH4 üretiminde en düşük değer %16.98 ile kontrol grubunda bulunurken; en yüksek üretim %24.31 ile yonca kuru otuna 1.8 ml atık kahve ekstraktı ilave edilen grupta bulunmuştur. Atık kahve ekstraktı ilavesi ile amonyak azotu (NH3-N) üretim miktarları 19.27 ile 23.77 mg 100ml-1 arasında değişmiş olup en düşük değer kontrol grubunda en yüksek değer ise 1.2 ml ilave edilen grupta bulunmuştur. Atık kahve ekstraktı ilavesinin yonca kuru otunun NH3-N üretim miktarlarını azalttığı görülmüştür (P<0.05). Metabolize edilebilir enerji ve in vitro organik madde sindirimi düzeylerini önemli seviyede etkilemiştir (P<0.001). Fermantasyon sonrası rumen sıvılarının pH değerlerinde kontrol grubuna oranla herhangi bir farklılık görülmemiştir (P>0.05). Sonuç olarak yonca kuru otuna atık kahve ekstraktı ilavesinin ruminant hayvanlarda antimetanojenik bir etki göstermediği görülmüştür.

Anahtar Kelimeler

in vitro, atık kahve ekstrakt, metan üretimi

Determination of Antimethanogenic Effect of Addition of Waste Coffee Extract to Alfalfa Hay (Medicago sativa) by In Vitro Gas Production Method

Öz

In this study, the addition of waste coffee extract at different doses to alfalfa hay (Medicago S.) harvested during flowering; fermentation parameters of alfalfa hay; The effects on pH values, ammonia nitrogen (NH3-N) production amounts, metabolizable energy and in vitro organic matter digestion were determined. The addition of waste coffee extract significantly affected the in vitro gas productions and methane production values of alfalfa hay (P<0.001). The 24-hour gas production amounts determined by the in vitro gas production method were found to be between 44.76 and 62.52 ml 200mg-1 dry matter (DM). Gas production values of alfalfa hay increased significantly with the addition of waste coffee extract, and the highest value was found in the group with 1.2 ml addition. While the lowest value in % CH4 production after fermentation was found in the control group with 16.98%; The highest production was found in the group that added 1.8 ml of waste coffee extract to alfalfa hay with 24.31%. Production amounts of ammonia nitrogen (NH3-N) with the addition of waste coffee extract varied between 19.27 and 23.77 mg 100ml-1, and the lowest value was found in the control group and the highest value in the group with 1.2 ml added. It was observed that the addition of waste coffee extract decreased the NH3-N production amounts of alfalfa hay (P<0.05). It significantly affected the levels of metabolizable energy and in vitro organic matter digestion (P<0.001). No difference was observed in the pH values of the rumen fluids after fermentation compared to the control group (P>0.05). As a result, it was observed that the addition of waste coffee extract to alfalfa hay does not have an antimethanogenic effect in ruminant animals.

Anahtar Kelimeler

in vitro, waste coffee extract, methane production

Kaynakça

• AOAC 1990. Official method of analysis. 15th ed., pp.66-88. Association of Official Analytical Chemists, Washington, DC, USA
• Goel G, Makkar HP, Becker K 2008. Effects of Sesbania sesban and Carduus pycnocephalus leaves and Fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage-and concentrate-based feeds to methane Anim Feed Sci Technol 147(1-3): 72-89.
• ICO 2019 International Coffee Organization Statistics. Int Coffee Organ. http://www.ico.org/trade_statistics.asp. Accessed 05/07/2019
• IPCC (Intergoverment Panel on Climate Change) 2001. Climate change 2001. The scientific basis. Cambridge University Press, Cambridge, UK.
• Jiménez-Peralta, F. S., Salem, A. Z. M., Mejia-Hernández, P., González-Ronquillo, M., Albarrán-Portillo, B., Rojo-Rubio, R., & Tinoco-Jaramillo, J. L. (2011). Influence of individual and mixed extracts of two tree species on in vitro gas production kinetics of a high concentrate diet fed to growing lambs. Livestock Science, 136(2-3), 192-200.
• Kılıç, Ü., Abdıwalı, M. A., 2016. Alternatif kaba yem kaynağı olarak şarapçılık endüstrisi üzüm atıklarının in vitro gerçek sindirilebilirlikleri ve nispi yem değerlerinin belirlenmesi. Kafkas Universitesi Veteriner Fakultesi Dergisi, 22(6).
• Lila, Z. A., Mohammed, N., Kanda, S., Kamada, T., and Itabashi, H. 2003. Effect of sarsaponin on ruminal fermentation with particular reference to methane production in vitro. Journal of Dairy Science, 86(10), 3330-3336.
• Menke, K. H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal research and development, 28, 7-55.
• Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D., and Schneider, W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science, 93(1), 217-222.
• Öztürk, H., 2008. Ruminant beslemesinde probiyotik mayalar. Veteriner Hekimler Derneği Dergisi., 79:3 37-42
• Patra, A. K., Kamra, D. N., & Agarwal, N. (2006). Effect of plant extracts on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Animal Feed Science and Technology, 128(3-4), 276-291.
• Salem, A. Z. M. (2012). Oral administration of leaf extracts to rumen liquid donor lambs modifies in vitro gas production of other tree leaves. Animal feed science and technology, 176(1-4), 94-101.
• Salem, A. Z., Kholif, A. E., Olivares, M., Elghandour, M. M., Mellado, M., & Arece, J. (2014). Influence of S. babylonica extract on feed intake, growth performance and diet in vitro gas production profile in young lambs. Tropical Animal Health and Production, 46(1), 213-219.
• Salinas-Rios, T., Ortega-Cerrilla, M. E., Sánchez-Torres-Esqueda, M. T., Hernández-Bautista, J., Díaz-Cruz, A., Figueroa-Velasco, J. L., ... and Cordero-Mora, J. L. 2015. Productive performance and oxidative status of sheep fed diets supplemented with coffee pulp. Small Ruminant Research, 123(1), 17-21.
• Selçuk, B. ve Kamalak, A. 2022. Biberiye Yaprağı Ekstraktının Yonca Otunun Rumen Fermantasyonu, Metan ve Mikrobiyal Protein Üretimine Etkisi, 25(3), 623-628. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi.
• Seo, J., Jung, J. K., and Seo, S. 2015. Evaluation of nutritional and economic feed values of spent coffee grounds and Artemisia princeps residues as a ruminant feed using in vitro ruminal fermentation. PeerJ, 3, e1343.
• Śliwiński, B. J., Soliva, C. R., Machmüller, A., and Kreuzer, M. 2002. Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Animal Feed Science and Technology, 101(1-4), 101-114.
• SPSS, SPSS for Windows advanced statistics release. IBM, Chicago, IL, USA 2016.
• TCI 2016 A brewing storm: the climate change risks to coffee. The Climate Institute. http://www.climateinstitute.org.au/coffee.html. Accessed 05/07/2019
• Teferedegne, B. 2000. New perspectives on the use of tropical plants to improve ruminant nutrition. Proceedings of the Nutrition Society, 59(2), 209-214.
• Van Lier JB, Mahmoud N, Zeeman G 2008. Anarobic Wastewater Treatment. pp.401-442. Biological Wastewater Treatment: Principles, Modeling and Design. Edit. Henze M, van Loosdrecht MCM, Ekama GA, Brdjanovic D. IWA Publishing, London UK.
• Van Soest, P. V., Robertson, J. B., Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of dairy science, 74(10), 3583-3597.
• Venkateswarlu, S., Radhakrishnan, L., Karunakaran, R., and Parthiban, M. 2020. Evaluation of spent coffee waste as a ruminant feed by in vitro gas production technique. IJCS, 8(2), 873-876.
• Vignoli, J. A., Bassoli, D. G., and Benassi, M. D. T. 2011. Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: The influence of processing conditions and raw material. Food chemistry, 124(3), 863-868.