Ratlarda Farklı Detomidin Kombinasyonlarının Klinik Olarak Karşılaştırılması

Abstract

Bu çalışmada, ratlar üzerinde detomidin, detomidin-ketamin, detomidin-ketamin-butorfanol ve detomidin-ketamin-diazepam kombinasyonlarının klinik olarak etkileri karşılaştırıldı. Toplam 32 rat dört farklı gruba ayrıldı. Her anestezi grubunda cinsiyet dağılımı eşit olacak şekilde (4 erkek, 4 dişi) 8 adet rat yer aldı. 1. Grup D’ye (n=8) 0,1 mg/kg detomidin; 2. DK’ye (n=8) 0,1 mg/kg detomidin ve 75 mg/kg ketamin; 3.Grup DKB’ye (n=8) 0,1 mg/kg detomidin, 75 mg/kg ketamin ve 5 mg/kg butorfanol; 4. Grup DKD’ye (n=8) 0,1 mg/kg detomidin, 75 mg/kg ketamin ve 5 mg/kg diazepam intraperitoneal yolla uygulandı. Anestezi öncesi ve anestezi sonrası 5, 10, 15, 20, 25, 30, 45, 60, 90 ve 120 dakika kardiyopulmoner parametreler (kalp frekansı, solunum frekansı, vücut sıcaklığı, perifer oksijen satürasyonu) ve refleksler (pedal, palpebral, korneal, ayak parmak arası) her dört grupta incelendi. Cerrahi anestezi süresi ve toplam anestezi süresi bakımından D grubuna göre DK, DKB ve DKD grupları anlamlı düzeyde düşük (P=0,000) bulundu. Refleks parametreleri bakımından D grubuna göre pedal, palpebral ve korneal refleks kaybolma süreleri DKD grubu (P=0,035), ayak parmak arası refleksi kaybolma süresi DKD grubu (P=0,023) anlamlı düzeyde düşük bulundu. Kalp frekansı, perifer oksijen satürasyonu ve vücut sıcaklığı bakımından her dört grupta da düşüşler olduğu görüldü. Sonuç olarak, D grubu ratlarda tek başına kullanıldığında yeterli sedasyon ve anestezi oluşturmadığı tespit edildi. DKB grubu iyi bir analjezi sağladığından ağrılı özellikle ortopedik cerrahi operasyonlarda, DKD grubu ise uzun süreli anestezi sağladığından cerrahi operasyonlarda kullanımı önerilmektedir.

Keywords

• rat
• anestezi
• solunum frekansı
• kalp frekansı
• detomidine

Clinical Comparison of Different Detomidine Combinations in Rats

Abstract

In this study, the clinical effects of detomidine, detomidine-ketamine, detomidine-ketamine-butorphanol, and detomidine-ketamine-diazepam combinations were compared in rats. A total of 32 rats were divided into four distinct groups. Each anesthesia group consisted of 8 rats with an equal sex distribution (4 males, 4 females). The experimental protocols were administered intraperitoneally as follows: Group D (n=8) received 0.1 mg/kg detomidine; Group DK (n=8) received 0.1 mg/kg detomidine and 75 mg/kg ketamine; Group DKB (n=8) received 0.1 mg/kg detomidine, 75 mg/kg ketamine, and 5 mg/kg butorphanol; and Group DKD (n=8) received 0.1 mg/kg detomidine, 75 mg/kg ketamine, and 5 mg/kg diazepam. Cardiopulmonary parameters (heart rate, respiratory rate, body temperature, peripheral oxygen saturation) and reflexes (pedal, palpebral, corneal, and interdigital) were evaluated in all four groups before anesthesia and at 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 minutes post-anesthesia. In terms of surgical and total anesthesia duration, the DK, DKB, and DKD groups were found to be significantly lower compared to Group D (P=0.000). Regarding reflex parameters, the disappearance times for pedal, palpebral, and corneal reflexes in the DKD group (P=0.035) and the interdigital reflex in the DKD group (P=0.023) were significantly shorter than those in Group D. Decreases in heart rate, peripheral oxygen saturation, and body temperature were observed across all four groups. In conclusion, it was determined that detomidine alone (Group D) did not provide adequate sedation or anesthesia in rats. The DKB combination is recommended for painful procedures, particularly orthopedic surgeries, due to its effective analgesia, while the DKD combination is suggested for surgical operations requiring prolonged anesthesia.

Keywords

• rat
• anaesthesia
• respiratory rate
• hearth rate
• detomidine

Ethical Statement

This research was supported by Aydın Adnan Menderes University Scientific Research Projects Unit with project number VTF-23016.

Thanks

This study was carried out with the approval of Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (ADU-HADYEK) dated 13.04.2023 and numbered 64583101/2023/53.

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