Evaluation of the Relationship Between Cervical Intraepithelial Neoplasia Grades and Connexin 43

Year 2017, , 15 - 19, 28.12.2017

Havva Erdem , Mürüvvet Akçay Çelik

https://doi.org/10.19127/mbsjohs.332613

Abstract

Objective: Cervical intraepithelial neoplasia (CIN) is a
premalign cervical disease. The microscopic features of CIN indicates an
alteration leading to dedifferentiation and loss of maturation in the squamous
epithelium.

Disruption of connexins are frequently reported
in malignant cell lines. Here, it is aimed to show the relationship between the
dysplasia grades and connexin.

Methods: 79 cases were included in the study who were
referred to the pathology department between 2014 and 2015 and who had CIN
(grade 1, 2, 3). Sections of 3 micrometer thickness were taken from the
paraffin blocks of the uterus on the polylysine slide. Cx43 antibody with ABC tecnique
were performed to these sections. Staining cells were defined as positive. The
cases were graded according to the intensity of the staining.

Results: The distribution of 79 dysplastic cases was as
follows. 41 of these cases had CIN 1. The average age of these women was 44.93.
16 women were diagnosed with CIN2. The average age of these women was 42.06. 22
women were diagnosed with CIN 3. The average age of the women was 48.87.

Conclusion: In this study, complete loss of Cx43 expression
was observed in all dysplastic cervical cases.

Keywords

Cervical intraepithelial neoplasia, degree of dysplasia, Cx43

References

• References Parkin DM, Bray F, Ferlay J, Pisani P. Global cancerstatistics, 2002. CA Cancer J Clin. 2005;55(2):74108. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of world wide burden of cancer in 2008: Globocan 2008. Int J Cancer. 2010;127(12): 2893-917. Atasü T, Aydınlı K. Jinekoloji ve obstetrik pratiğinde kolposkopi. Jinekolojik Onkoloji.1996; 12:182. Hong R, Lim SC. Pathological significance of connexin 26 expression in colorectal adenocarcinoma .Oncology Reports. 2008; 19: 913-919. Beyer EC, Paul DL, Goodenough DA: Connexin 43: A protein from rat heart homologous to a gap junction protein from liver. J Cell Biol. 1987; 105: 2621-2629. Beyer EC, Davis LM, Saffitz JE, Veenstra RD, “Cardiac intercellular communication: consequences of connexin distribution and diversity,” Brazilian Journal of Medical and Biological Research.1995; 28(4):415–425. El aoumari a, Fromaget C, Dupont E, Reggio H, Durbec P, Briand JP et al., “Conservation of a cytoplasmic carboxy-terminal domain of connexin 43, a gap junctional protein, in mammal heart and brain,” The Journal of Membrane Biology.1990;115( 3):229–240. King TJ, Fukushima LH, Hieber AD, Shimabukuro KA, Sakr WA, Bertram JS. Reduced levels of connexin 43 in cervical dysplasia: inducible expression in a cervical carcinoma cell line decreases neoplastic potential with implications for tumor progression. Carcinogenesis.2000;(21)6:1097–1109. Nicolson GL, Dulski KM, Trosko JE. “Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells,” Proceedings of the National Academy of Sciences of the United States of America.1988;(85)2;473–476. Carystinos GD, Bier A, Batist G. “The role of connexinmediated cell-cell communication in breast cancer metastasis,” Journal of Mammary Gland Biology and Neoplasia.2001;(6)4;431–440. Bišćanin A, Ljubičić N, Boban M, Baličević D, Pavić I, Bišćanin MM et al. Cx43 expression in colonic adenomas and surrounding mucosa ıs a marker of malignant potential. Anticancer Research. 2016(36)10 ;5437-5442. Hieber AD, King TJ, Morioka S, Fukushima LH, Franke AA, Bertram JS. Comparative effects of all-trans beta-carotene vs. 9-cis beta-carotene on carcinogen-induced neoplastic transformation and connexin 43 expression in murine 10T1/2 cells and on the differentiation of human keratinocytes.Nutr. Cancer . 2000; 37:234–244. Sutherland BM, Bennett PV. Transformation of human cells by DNA transfection. Cancer Res. 1984;44: 2769–2772. King, TJ, Fukushima, LH, Yasui Y, Lampe P.D, Bertram JS. Mol. Carcinog. 2002;35:29–41. Bertram JS. Dietary carotenoids, connexins and cancer: what is the connection?. Biochemical Society Transactions.2004;32(6): 985-9. Puzzo L, Caltabiano R, Parenti R, Trapasso S, Allegra E. Connexin 43 (Cx43) Expression in Laryngeal Squamous Cell Carcinomas: Preliminary Data on Its Possible Prognostic Role. Head Neck Pathol. 2016;10(3):292-7. Wilgenbus KK, Kirkpatrick CJ, Knuechel R, Willecke K, Traub O. Expression of Cx26, Cx32 and Cx43 gap junction proteins in normal and neoplastic human tissues. Int J Cancer. 1992;51(4):522-9. Tada J, Hashimoto K. Ultrastructural localization of gap junction protein connexin 43 in normal human skin, basal cell carcinoma, and squamous cell carcinoma. J Cutan Pathol. 1997 ;24(10):628-35. Schneider B, Teschner M, Sudermann T, Pikula B, Lautermann J. Expression of gap junction proteins (connexin 26, 30, 32, 43) in normal mucosa, hyperkeratosis and carcinoma of the human larynx. ORL J Otorhinolaryngol Relat Spec. 2002;64(5):324-9. Hagemann IS, Pasha TL, Roberts SA, Yum SW, Zhang PJ. Aberrant connexin 43 and 26 expression in cervical dysplasia.Analytical and Quantitative Cytology and Histology. 2012; 34(1):28-40.