miR-1267 Induces Tumorigenicity and Contributes to Risk of Clear Cell Renal Cell Carcinoma

Year 2019, , 100 - 105, 28.08.2019

Sercan Ergün Kalbiye Konanç

https://doi.org/10.19127/mbsjohs.569404

Abstract

Objective: Dysregulated microRNA signatures in different cancer
types are being uncovered continually implying their significance in cancer
pathogenesis. miR-1267 was not previously associated with RCC. In this
study, it is aimed to obtain the expression profile of miR-1267 in patients
with ccRCC and its correlation with patient parameters.

Methods:
Kidney Cancer cDNA Array consisting of cDNA samples obtained from healthy
kidney tissues of 4 healthy individuals and tumoral kidney tissues of 5 Stage
I, 5 Stage II, 3 Stage III and 2 Stage IV ccRCC patients was used. Hsa-miR-1267
and SNORD48 (as housekeeping gene) expressions were analyzed. miR-1267
expression was statistically correlated with the clinical parameters of
patients. miRGator 3.0 database was used to compare miR-1267 expression
patterns of different urological cancer types.

Results:
The expression of miR-1267 was significantly higher in male than female
(p=0.027). Also, there were statistically significant increase in miR-1267
expression in stage IV when compared to stage I (p<0.001). Moreover,
increased platelet/lymphocyte ratio and calcium level, which were parameters
giving information about the occurrence of ccRCC, are significantly associated
with increased miR-1267 expression (p<0.001 and p=0.003, respectively). The expression of miR-1267 in kidney tumor tissues was higher
approximately three times than normal kidney tissues (p>0.05).

Conclusion: miR-1267 could have
oncogenic function, have predictive value for RCC development and be predictive
about aggressiveness in ccRCC.

Keywords

miR-1267, ccRCC, platelet/lymphocyte ratio, high calcium level

Supporting Institution

Scientific Research Projects Commission of Ordu University

Project Number

HD-1718

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