The Role of Hypericum Perforatum (St. John's Wort Oil) and Olive Oil in the Prevention of Peritoneal Adhesion in a Rat Model: An Animal Study

Year 2022, Volume: 8 Issue: 1, 23 - 30, 25.02.2022

Tuğrul Kesicioğlu , İsmail Aydın , Selahattin Vural , İlkay Çinar , Mehmet Gülmez , Arif Keskin

https://doi.org/10.19127/mbsjohs.1008275

Abstract

Objective: We aimed to investigate Hypericum Perforatum (St. John's Wort Oil) and pure olive oil with respect to their effect on preventing intra-abdominal adhesions after abdominal surgery.
Methods: This was an animal study carried out by the Giresun Faculty of Medicine, Department of General Surgery between August 10, 2020, and September 10, 2020. In the study, intra-abdominal adhesion development was examined macroscopically and microscopically on the 14th day after experimental abdominal surgery in three groups [(i) Control, (ii) olive oil application, (iii) St. John's Wort oil application] each consisting of 7 female Wistar Albino rats. Majuzi classification and Zuhlke's microscopic adhesion classification was used to evaluate adhesions.
Results: Macroscopically, fibrous bands were not observed in 1 rat in the control group, 2 rats in olive oil recipients, and 1 rat in St. John's Wort oil recipients. A significant difference between the groups was identified in terms of Zuhlke's microscopic adhesion scores (p = 0.026). Accordingly, the adhesion level was significantly reduced in olive oil recipients compared to controls (p = 0.002). There was no significant difference in terms of adhesion between all groups (p>0.05). The Majuzi adhesion classifications in the control group, olive oil group and St. John's wort groups were also similar (p = 0.308).
Conclusion: In rats who underwent abdominal surgery, it was determined that intra-abdominal olive oil application reduced the level of adhesion, while St. John's Wort oil application had no effect on adhesion formation.

Keywords

Abdominal surgery, adhesion, St. John, olive oil

References

• 1. Dubuisson J, Botchorishvili R, Perrette S, Bourdel N, Jardon K, Rabischong B, et al. Incidence of intraabdominal adhesions in a continuous series of 1000 laparoscopic procedures. Am J Obstet Gynecol. 2010;203(2):111.e1-3.
• 2. Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Ann Med Surg (Lond). 2017;15:9-13.
• 3. Bruggmann D, Tchartchian G, Wallwiener M, Munstedt K, Tinneberg HR, Hackethal A. Intra-abdominal adhesions: definition, origin, significance in surgical practice, and treatment options. Dtsch Arztebl Int. 2010;107(44):769-75.
• 4. Uslu Yuvaci H, Cevrioglu AS, Gunduz Y, Akdemir N, Karacan A, Erkorkmaz Ü, et al. Does applied ultrasound prior to laparoscopy predict the existence of intra-abdominal adhesions? Turk J Med Sci. 2020;50(2):304-11.
• 5. Maciver AH, McCall M, James Shapiro AM. Intra-abdominal adhesions: cellular mechanisms and strategies for prevention. Int J Surg. 2011;9(8):589-94.
• 6. Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011;17(41):4545-53.
• 7. Urkan M, Özerhan İ H, Ünlu A, Can MF, Özturk E, Gunal A, et al. Prevention of Intraabdominal Adhesions: An Experimental Study Using Mitomycin-C and 4% Icodextrin. Balkan Med J. 2017;34(1):35-40.
• 8. Oh J, Kuan KG, Tiong LU, Trochsler MI, Jay G, Schmidt TA, et al. Recombinant human lubricin for prevention of postoperative intra-abdominal adhesions in a rat model. J Surg Res. 2017;208:20-5.
• 9. Ng QX, Venkatanarayanan N, Ho CY. Clinical use of Hypericum perforatum (St John's wort) in depression: A meta-analysis. J Affect Disord. 2017;210:211-21.
• 10. Huang N, Rizshsky L, Hauck C, Nikolau BJ, Murphy PA, Birt DF. Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages. Phytochemistry. 2011;72(16):2015-23.
• 11. Suntar I, Oyardı O, Akkol EK, Ozcelik B. Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation. Pharm Biol. 2016;54(6):1065-70.
• 12. Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol. 2010;131(3):511-21.
• 13. Russo E, Scicchitano F, Whalley BJ, Mazzitello C, Ciriaco M, Esposito S, et al. Hypericum perforatum: pharmacokinetic, mechanism of action, tolerability, and clinical drug-drug interactions. Phytother Res. 2014;28(5):643-55.
• 14. Hajhashemi M, Ghanbari Z, Movahedi M, Rafieian M, Keivani A, Haghollahi F. The effect of Achillea millefolium and Hypericum perforatum ointments on episiotomy wound healing in primiparous women. J Matern Fetal Neonatal Med. 2018;31(1):63-9.
• 15. Schwingshackl L, Christoph M, Hoffmann G. Effects of Olive Oil on Markers of Inflammation and Endothelial Function-A Systematic Review and Meta-Analysis. Nutrients. 2015;7(9):7651-75.
• 16. Gavahian M, Khaneghah AM, Lorenzo JM, Munekata PE, Garcia-Mantrana I, Collado MC, et al. Health benefits of olive oil and its components: Impacts on gut microbiota antioxidant activities, and prevention of noncommunicable diseases. Trends Food Sci Technol. 2019;88:220-7.
• 17. Cicerale S, Lucas LJ, Keast RS. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Curr Opin Biotechnol. 2012;23(2):129-35.
• 18. Yildiz T, Ilce Z, Yildirim M, Akdogan M, Yurumez Y, Varlikli O, et al. Antienflamatuar and antiadhesive effect of clioquinol. Int J Surg. 2015;15:17-22.
• 19. Bayhan Z, Zeren S, Kocak FE, Kocak C, Akcılar R, Kargı E, et al. Antiadhesive and anti-inflammatory effects of pirfenidone in postoperative intra-abdominal adhesion in an experimental rat model. J Surg Res. 2016;201(2):348-55.
• 20. Sosa S, Pace R, Bornancin A, Morazzoni P, Riva A, Tubaro A, et al. Topical anti-inflammatory activity of extracts and compounds from Hypericum perforatum L. J Pharm Pharmacol. 2007;59(5):703-9.
• 21. Cakir A, Mavi A, Yildirim A, Duru ME, Harmandar M, Kazaz C. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. J Ethnopharmacol. 2003;87(1):73-83.
• 22. Hizli D, Hizli F, Kosus A, Yilmaz S, Kosus N, Haltas H, et al. Effect of Hypericum perforatum on intraperitoneal adhesion formation in rats. Arch Med Sci. 2014;10(2):396-400.
• 23. Schanuel FS, Saguie BO, Monte-Alto-Costa A. Olive oil promotes wound healing of mice pressure injuries through NOS-2 and Nrf2. Appl Physiol Nutr Metab. 2019;44(11):1199-208.
• 24. Ural DA, Saruhan H, Saygın I, Aykan DA, Ural A, Imamoglu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-22.
• 25. de la Puerta R, Martínez-Domínguez E, Ruíz-Gutiérrez V. Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z Naturforsch C J Biosci. 2000;55(9-10):814-9