Sirotik Karaciğer Hastalarında Bazı Antioksidan Aktivitelerin (Süperoksit Dismutaz, Katalaz, İndirgenmiş Glutatyon) ve Oksidatif Stres Düzeyinin (Malondialdehit Asit) Belirlenmesi

Year 2022, Volume: 8 Issue: 4, 506 - 514, 30.11.2022

Hamam Hamam , Halit Demir , Mesut Aydın , Canan Demir

https://doi.org/10.19127/mbsjohs.1108726

Cited By: 2

Abstract

Amaç: Bu çalışmanın amacı karaciğer sirozlu hastaların kan serumunda lipid peroksidasyon (MDA) ve indirgenmiş glutatyon (GSH), katalaz (CAT) ve süperoksit dismutaz (SOD) gibi antioksidanların düzeylerini belirlemektir.
Yöntemler: Bu çalışmada serum malondialdehit asit (MDA) düzeyleri, süperoksit dismutaz (SOD), indirgenmiş glutatyon (GSH) ve katalaz (CAT) aktiviteleri spektrofotometrik olarak ölçüldü.
Bulgular: Hasta gruplarında SOD, CAT ve GSH aktiviteleri sağlıklı kontrol grubuna göre anlamlı olarak azaldı (p<0.05). MDA düzeyleri hasta grubunda sağlıklı kontrol grubuna göre anlamlı olarak yüksekti (p<0.05).
Sonuç: Sonuç olarak, bu çalışmada oksidatif stres karaciğer sirozu gelişiminde önemli rol oynayabilir. Bu çalışma karaciğer sirozunda MDA, SOD, CAT ve GSH ilişkilerini gösteren ilk çalışmadır. Karaciğer sirozunda antioksidan enzimleri ve oksidatif stres durumunu araştırmak için daha ileri çalışmalar gereklidir.

Keywords

Karaciğer Sirozu, SOD, GSH, CAT, MDA

Determination of some Antioxidant Activities (Superoxide Dismutase, Catalase, Reduced Glutathione) and Oxidative Stress Level (Malondialdehyde Acid) in Cirrhotic Liver Patients

Abstract

Objective: The aim of this study was to determine the levels of malondialdehyde (MDA) and antioxidants such as reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) in the blood serum of liver cirrhosis patients.
Methods: İn This investigation, we took blood from 31 healthy individuals, and 30 patients with Cirrhosis in both males and females. In this study, serum MDA levels, SOD, GSH, and CAT activities were measured spectrophotometrically. In paired group comparisons in terms of continuous variables; the T-test was utilized where normal deviation was achieved, and Mann-Whitney U statistics was utilized where it was not. In addition, ROC curve analysis was performed to evaluate their performance in differentiating the patient group from the control group.
Results: SOD, CAT, and GSH activities were significantly decreased in the patient groups compared to the healthy control group (p<0.05). MDA levels were significantly higher in the patient group compared to the healthy control group (p <0.05).
Conclusion: In conclusion, in this study, oxidative stress may play an important role in the development of liver cirrhosis. This study is the first one to show the relationships of MDA, SOD, CAT, and GSH in liver cirrhosis. Further studies are essential to investigate antioxidant enzymes and oxidative stress status in liver cirrhosis.

Keywords

Liver Cirrhosis, SOD, GSH, CAT, MDA

References

• 1. Tiwari AK. Antioxidants: new-generation therapeutic base for treatment of polygenic disorders. Curr Sci. 2004; 86:1092.
• 2. Wood-Kaczmar A, Gandhi S, Wood NW. Understanding the molecular causes of Parkinson's disease. Trends in molecular medicine. 2006;12(11):521-528.‏
• 3. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes care. 1996;19(3):257-267.‏
• 4. Khan MA, Tania M, Zhang DZ, Chen HC. Antioxidant enzymes and cancer. Chinese Journal of Cancer Research. 2010;22(2): 87-92.‏
• 5. Schaffner F, Popper H. Capillarization of hepatic sinusoids in man. Gastroenterology. 1963;44(3):239-242.‏
• 6. Wanless IR, Nakashima E, Sherman M. Regression of human cirrhosis: morphologic features and the genesis of incomplete septal cirrhosis. Archives of pathology & laboratory medicine. 2000;124(11):1599-1607.
• 7. Desmet VJ, Roskams T. Cirrhosis reversal: a duel between dogma and myth. Journal of hepatology. 2004;40(5):860-867.‏
• 8. Popov B, Gadjeva V, Valkanov P, Popova S, Tolekova A. Lipid peroxidation, superoxide dismutase and catalase activities in brain tumor tissues. Archives of physiology and biochemistry, 2003;111(5): 455-459.‏
• 9. Aebi H. Catalase In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, Verlag Chemie/Academic Press Inc., Weinheim/NewYork, 1974; 673-680.
• 10. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 1963;61:882–888.
• 11. Gutteridge JM. Lipit peroxidation and antioxidants as biomarkers of tissue damage, Clin. Chem 1995;41(12):1819–1828.
• 12. Salman KA, Ashraf S. Reactive oxygen species: A link between chronic inflammation and cancer. Asia-Pacific Journal of Molecular Biology and Biotechnology. 2013;21(2):42-49.
• 13. Cichoż-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World journal of gastroenterology: WJG, 2014;20(25):8082.
• 14. Salganik RI. The benefits and hazards of antioxidants: controlling apoptosis and other protective mechanisms in cancer patients and the human population. Journal of the American College of Nutrition. 2001;20(sup5):464S-472S.‏
• 15. Cerutti PA. Oxy-radicals and cancer. The Lancet. 1994;344(8926):862-863.‏
• 16. Moncada S, Higgs A. The L-arginine- nitric oxide pathway. N Engl J Med. 1993;329:2002-12.
• 17. Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB. Resources and biological activities of natural polyphenols. Nutrients. 2014;6(12):6020-6047.‏
• 18. Babczyńska A, Wilczek G, Migula P. Effects of dimethoate on spiders from metal pollution gradient. Science of the total environment. 2006;370(2-3):352-359.‏
• 19. Mallikarjuna K, Shanmugam KR, Nishanth K, Wu MC, Hou CW, Kuo CH, Reddy KS. Alcohol-induced deterioration in primary antioxidant and glutathione family enzymes reversed by exercise training in the liver of old rats. Alcohol. 2010;44(6):523-529.‏
• 20. Shanmugam KR, Mallikarjuna K, Reddy KS. Effect of alcohol on blood glucose and antioxidant enzymes in the liver and kidney of diabetic rats. Indian journal of pharmacology. 2011;43(3):330.‏
• 21. Videla LA, Rodrigo R, Orellana M, Fernandez V, Tapia G, Quinones L, et al. Oxidative stress-related parameters in the liver of non-alcoholic fatty liver disease patients. Clinical science. 2004;106(3):261-268.
• 22. Das SK, Balakrishnan V, Mukherjee S, Vasudevan DM. Evaluation of blood oxidative stress-related parameters in alcoholic liver disease and non-alcoholic fatty liver disease. Scandinavian journal of clinical and laboratory investigation. 2008;68(4):323-334.‏
• 23. Singal AK, Jampana SC, Weinman SA. Antioxidants as therapeutic agents for liver disease. Liver International. 2011;31(10):1432-1448.‏
• 24. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y. The role of oxidative stress and antioxidants in liver diseases. International journal of molecular sciences. 2015;16(11): 26087-26124.‏
• 25. Moncada S, Higgs EA. Endogenous nitric oxide: physiology, pathology, and clinical relevance. European Journal of Clinical Investigation. 1991;21:361-374.
• 26. Kalpakcioglu B, Senel K. The interrelation of glutathione reductase, catalase, glutathione peroxidase, superoxide dismutase, and glucose-6-phosphate in the pathogenesis of rheumatoid arthritis. Clinical Rheumatology. 2008;27:141-145. doi:10.1007/s10067-007-0746-3.
• 27. Abrahamovych M, Abrahamovych O, Fayura O, Fayura L, Tolopko S. The effect of oxidative stress on the autonomic nervous system in patients with liver cirrhosis. Georgian Med News. 2020;298:94-99.