Lipid Peroxidation In Patients With Multipl Myeloma

Year 2000, Volume: 1 Issue: 3, 13 - 15, 01.12.2000

Zahit Bolaman Süleyman Demir Mehmet H. Köseoğlu Yaşar Enli Gürhan Kadıköylü Diler Aslan

Abstract

Aim of study: We investigated the malondialdehyde (MDA) levels as a parameter of lipid peroxidation in patients with multiple myeloma as a preliminary report. Material and method: Fasting venous blood samples were drawn in 12 patients with multiple myeloma and 15 healthy persons. Serum was separated and frozen at -20 C°. All samples were thawed at room temperature and MDA levels were measured by the method in which MDA reacted with thiobarbituric acid and produced a colored complex. This colored complex was read by optic scale at 532 wavelength. Results: MDA levels were 1.51±0.16nmol/ml in patients withmultiple myeloma and 0.97±0.11 nmol/mlinthe control group (P<0.05). We didn't observe a significant relationship between the concentration of MDA and age, sex, paraprotein level, lightchaintype and hemoglobin levels (p>0.05). Conclusion: Plasma MDA levels were found to be higher in multiple myeloma in comparison to control group (p<0.05). These results are a preliminary report and the higher plasma MDA levels could be an indicator of increased oxidative stress in multiple myeloma.

Keywords

Multiple myeloma, malondialdehyde, oxidative stress

References

• 1. Barlogie B, Epstein J, Selvanayagam P, Alexanian R. Plasma cell myeloma-new biological insight and advances in therapy. Blood 1989; 73:865-79.
• 2. Hallek M, Bergsagel PL, Anderson KC. Multiple myeloma:increasing evidence for a multistep transformation process. Blood 1998; 91:3-21
• 3. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford Medicine Press, 1999; 246-351.
• 4. Allen RG, Venkatraj VS. Oxidants and antioxidants in development and differentiation. J Nutr 1992;122: 631- 5.
• 5. Tamer L, Polat G, Eskandari G, Ercan B, Atik U. Serbest Radikaller. Mersin Üniversitesi Týp Fakültesi Dergisi 2000; 1:52-8.
• 6. Ray C, Batra S, Shukla NK, Doo S, Raina V, Ashok S, Husain SA. Lipid peroxidation, free radical production and antioxidant status in breast cancer. Breast Cancer Res Treat 2000 59:163-70.
• 7. Cheeseman KH, Slater TF. An introduction to free radical biochemistry in vivo. British Medical Bulletin 1993; 49:481-93.
• 8. Janero DR. Malondialdehyde and thiobarbituric acidreactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990; 9:515-40.
• 9. Devi GS, Prasad MH, Saraswathi I, Raghu D, Rao DN, Reddy PP. Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias. Cli Chim Acta 2000; 293:53-62.
• 10. Ghalaut VS, Ghalaut PS, Singhs S. Lipid perxidation in leukemia. J Asssoc Physician India 1999; 47:403-5.
• 11. Güven M, Özturk B, Sayal A Özet A. Lipid peroxidation and antioxidant system nthe blood of patients with Hodgkin's disease. Clin Biochem 2000; 33:209-12.
• 12. Sabitha KE, Shymaladevi CS. Oxidant and antioxidant activity changes in patients with oral cancer and treated with radiotherapy. Oral Oncol 1999; 35:273-7.
• 13. Sadani GR, Nadkarni GD. Role of tissue antioxidant defence in thyroid cancers. Cancer Lett 1996; 3:231-5.
• 14. Iwagaki H, Hamazaki K, Matsubara N, Hiramatsu M, Orita K, Mori A. Lipid peroxidation in hepatocelluler carcinoma. Acta Med Okayama 1995; 49:313-5.
• 15. Zima T, Spicka I, Stipek S, Crkovska J, Platenik J, Merta M, Tesar V. Antioxidant enzymes and lipid peroxidation in patients with multiple myeloma. Neoplasma 1996; 43:69-73.
• 16. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-8.
• 17. Cheeseman KH. Mechanisms and effects of lipid peroxidation. Mol Aspect Med 1993; 14:191-7.
• 18. Li B, Gutierrez PL, Amstad P, Blough NV. Hydroxyl radical production by mouse epidermal cell line in the presence of quinone anti-cancer compounds. Chem Res Toxicol 1999; 12:1042-9.
• 19. Comporti M. Lipid peroxidation:Biopathological significance. Mol Asp Med 1993; 14:199-207.
• 20. Diplock AT, Rice-Evans CA, Burdon RH. Is there a significant role for lipid peroxidation in the causation of malignancy and for antioxidant in cancer prevention. Cancer Res 1994; 1:1952- 4.