ABDOMİNAL AORTA İSKEMİ- REPERFÜZYONUNA BAĞLI GELİŞEN AKCİĞER HASARINDA KARNOZİNİN ETKİSİ

Year 2010, Volume: 11 Issue: 3, 41 - 47, 16.12.2010

Bilge Alaçam Rauf Onur Ek Yüksel Yıldız Mukadder Serter Nezihe Tülün Boylu Sadun Temoçın

Abstract

AMAÇ:Aortanın geçici olarak klemplenmesini gerektiren cerrahi girişimlerden sonra, alt ekstremitelerdeiskemi/reperfüzyon (İR) hasarı ve akciğerlerde uzak organ hasarı oluşabilir. İR hasarında nötrofillerinaktivasyonunun önemli bir rol oynadığı bilinmektedir. Amacımız rat modelinde abdominal aortanınklemplenmesini takiben oluşan akciğer iskemi reperfüzyon hasarında karnozinin etkisini araştırmaktır.GEREÇ ve YÖNTEM:Yirmidört adet Sprague-Dawley cinsi rat, rasgele ve eşit sayıda (n=8) olmak üzere üçgruba ayrıldı. Grup 1 deki ratlarda batın açıldı ve abdominal aortları eksplore edildikten sonra hiçbir girişimyapılmadan tekrar kapatıldı. Grup 2'de abdominal aortaya klemp konuldu. 30 dakika iskemi süresinin sonundaklemp kaldırıldı ve 60 dakika reperfüzyon gerçekleştirildi. 2. grupdaki işlemin aynısı karnozin verilen 3. gruba dauygulandı ve karnozin intraperitoneal yolla klemp kaldırılmadan 10 dakika önce, 250 mg/kg olarak verildi. Tümgruplardaki ratlar reperfüzyon süresinin tamamlanmasıyla sakrifiye edildi. Histolojik inceleme ve biyokimyasalanalizler için akciğer doku örnekleri alındı.BULGULAR:Akciğer dokusunda Grup 2'ye ait malonil dialdehit, süperoksit dismutaz, myeloperoksidaz,okside glutatyon ve total glutatyon değerleri kontrol grubundaki değerlere göre anlamlı derecede yüksek bulundu(p<0,05). Grup 3'e ait malonil dialdehit ve okside glutatyon değerleri, aortik iskemi reperfüzyon grubundakideğerlere göre anlamlı derecede düşük bulundu (p<0,05). İmmünohistokimyasal değerlendirmede iNOS içinimmünreaktivitenin grup 1'de olmadığı, grup 2'de şiddetli düzeyde olduğu grup 3'de ise hafif düzeyde olduğugörüldü.SONUÇ:Karnozinin, abdominal aortanın geçici iskemi reperfüzyon hasarına bağlı olarak akciğerlerde oluşanpatolojik değişikleri önlemede yararlı etkileri bulunmaktadır

Keywords

İskemi reperfüzyon hasarı, akciğer, karnozin

The Effect of Carnosine on Lung Injury Induced by Abdominal Aortic Ischemia-Reperfusion

Abstract

OBJECTIVE: The purpose of this study is to examine the effect of carnosine on ischemia reperfusion injury in lungs occuring after occlusion reperfusion of infrarenal abdominal aorta in rats. MATERIALS and METHODS: Twentyfour Sprague-Dawley rats were randomly divided into 3 groups (n=8). Sham laparotomy (control) group (Group 1) underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. In aortic ischemia reperfusion groups (group 2 and 3), after laparotomy and exteriorization of the infrarenal abdominal aorta, aorta was occluded for 30 min ischemia and then the clamp was removed for 60 min reperfusion. In group 3; 250mg/kg carnosine, were given intraperitoneally 10 min before declamping aorta. The lung tissue specimens were taken for biochemical analysis and immunohistochemical examination. RESULTS: In lung specimens, malonil dialdehyde, oxidized glutathione and total glutathione levels, and superoxide dismutase and myeloperoxidase activities were significantly higher in the aortic ischemia reperfusion (group 2) than the sham laparotomy (control) group (p<0.05). Levels of malonil dialdehyde and oxidized glutathione were significantly lower in the aortic ischemia reperfusion and carnosine group (group 3) than the aortic ischemia reperfusion one (p<0.05). While iNOS immunoreactivities were severe in group 2 and mild in group 3, they were not observed in group 1. CONCLUSION: Carnosine was beneficial in prevention of lung injury after abdominal aortic occlusionreperfusion injury.

Keywords

Ischemia reperfusion injury, lung, carnosine

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