The Relationship Between Respiratory Function Tests and Serum Leptin IL-4, IL-10, IFN-ã Levels and Plasma Nitric Oxide Concentrations in Asthmatic Adults

Year 2013, Volume: 14 Issue: 1, 23 - 30, 01.04.2013

Mustafa Yılmaz Mustafa Altınışık Mehmet Polatlı Nimet Demirtaş Cengiz Gökbulut Orhan Çildağ

Abstract

PURPOSE: Asthma is accepted as a chronic inflammatory disease of the airway epithelium. In asthma, balance between Th1/Th2 lymphocytes is changed in favour of the Th2 lymphocytes. In this study, it was aimed to investigate the relationship between respiratory function tests and serum leptin, IL-4, IL-10, IFN-γ levels and plasma nitric oxide concentrations. MATERIAL and METHODS: In this study, 32 clinically asthma diagnosed patients were taken as a patient group and 32 healthy individuals as a control group. Respiratory function tests for each case were carried out with spirometer. Serum leptin, IL-4, IL-10, and IFN-γ levels were measured with a commercial kit that uses ELISA. Plasma nitrite and nitrate levels were detected with HPLC. Data were analysed with, Student t-test and Pearson correlation test by using SPSS 13.0 software. RESULTS: In control group, there was a positive correlation between leptin levels and FEV1 and FVC with the value of r=0,443 p=0,005 and r=0,440 p=0,013 respectively, whereas in patient group, there was a negative correlation between leptin levels and FEV1 with the value of r=-0,366 p=0,043. Serum leptin levels were significantly higher in women compared to men, p<0,001. In patient group, plasma nitric oxide levels were significantly lower compared to the control group, p=0,012. CONCLUSION: These findings suggest that, airway inflammation may have systemic effects in asthma so that leptin and nitric oxide levels might be useful parameters to detect these systemic effects.

Keywords

Asthma, leptin, nitrik oksit, IL-4, IL-10, IFN-ã

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• Uzm. Dr. Mustafa YILMAZ
• Yýl Milas Devlet Hastanesi, Biyokimya
• Laboratuvarý, MUÐLA, TÜRKÝYE E-Posta
• : [e-mail] Geliþ Tarihi Kabul Tarihi : 09.01.2013