THIOL/DISULPHIDE HOMEOSTASIS AND VASCULAR ENDOTHELIAL GROWTH FACTOR LEVELS IN SALIVA OF TYPE 1 DIABETIC CHILDREN WITH GINGIVITIS

Year 2024, Volume: 25 Issue: 4, 451 - 465, 22.12.2024

Sultan Keles , Burcin Irem Abas , Ahmet Anık , Ayşe Anık , Ozge Cevik

https://doi.org/10.69601/meandrosmdj.1596241

Abstract

Amaç: Tip 1 diyabeti ve gingivitisi olan hastaların tiyol/disülfit dengesini ve tükürük vasküler endotelyal büyüme faktörü (VEGF) düzeyini değerlendirmektir.
Gereç ve Yöntemler: Tip 1 diyabeti (DM) olan 40 ve sistemik olarak sağlıklı olan 40 çocuk (H) çalışmaya dahil edildi. Çocuklar, periodontal ve sistemik sağlık durumlarına göre dört alt gruba ayrıldılar: 1) sistemik ve periodontal olarak sağlıklı çocuklar (Hh), 2) sistemik olarak sağlıklı gingivitisli çocuklar (Hg), 3) tip 1 diyabeti olan periodontal olarak sağlıklı çocuklar (DMh), 4) tip 1 diyabeti olan gingivitisli çocuklar (DMg). Sondalama derinliği (PD), gingival indeks (PI) ve plak indeksi (PI) değerleri kaydedildi. Tiyol/disülfit dengesi parametrelerini ölçmek için otomatik ölçüm metodu, VEGF konsantrasyonlarını ölçmek için ise ELISA analizi kullanıldı.
Sonuçlar: DM ve H grupları benzer klinik periodontal parametrelere ve tükürük VEGF seviyelerine sahipti (p>0,05). GI, PI, PD ve disülfit ölçümleri, periodontal olarak sağlıklı alt gruplara (Hh ve DMh) kıyasla gingivitis alt gruplarında (Hg ve DMg) istatistiksel olarak anlamlı düzeyde daha yüksekti (p<0,001). Gingivitis alt grupları (Hg ve DMg), periodontal olarak sağlıklı alt gruplara (Hh ve DMh) kıyasla istatistiksel olarak anlamlı düzeyde daha yüksek VEGF miktarlarına sahipti (p<0,001).
Sonuçlar: Tiyol/disülfit dengesi, gingivitisli diyabetik çocuklarda disülfit yönüne doğru hareket eder. Tükürükteki tiyol/disülfit dengesi ve VEGF seviyeleri, gingival inflamasyonun tanısal belirteçleri olabilir.

Keywords

Tükürük, gingivitis, diabetes mellitus, oksidatif stres

THIOL/DISULPHIDE HOMEOSTASIS AND VASCULAR ENDOTHELIAL GROWTH FACTOR LEVELS IN SALIVA OF TYPE 1 DIABETIC CHILDREN WITH GINGIVITIS

Abstract

Objective: To evaluate the thiol/disulphide homeostasis and level of vascular endothelial growth factor (VEGF) in saliva of children with type 1 diabetes mellitus and gingivitis.
Methodology: Forty children with type 1 diabetes mellitus [DM] and 40 systemically healthy [H]) children were recruited for the study. Based on children’ periodontal and systemic health status, they were divided into four subgroups: 1) systemically and periodontally healthy children (Hh), 2) systemically healthy children with gingivitis (Hg), 3) diabetic children with periodontal health (DM+h), and 4) diabetic children with gingivitis (DM+g). Periodontal clinical parameters were recorded. A new automated technique was used to measure the thiol/disulphide homeostasis parameters, and ELISA was used to measure the amounts of VEGF in saliva.
Results: Children with or without diabetes had comparable clinical periodontal parameters and salivary VEGF levels (p>0.05). GI, PI, PD, and disulphide levels were significantly higher in the gingivitis subgroups than in the periodontally healthy subgroups (p<0.001). The gingivitis subgroups had significantly higher amounts of VEGF compared to the periodontally healthy subgroups (p<0.001).
Conclusions: Thiol/disulphide homeostasis shifts towards disulphide direction in diabetic children with gingivitis. Thiol/disulphide homeostasis and salivary VEGF levels may be diagnostic markers of gingival inflammation.

Keywords

saliva, gingivitis, diabetes mellitus, oxidative stress

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