Differences in the Differential Expression of MicroRNAs Between Patients with Familial Multiple Sclerosis and Those with Sporadic Multiple Sclerosis

Year 2023, Volume: 24 Issue: 4, 334 - 342, 30.12.2023

Halil Güllüoğlu , Hasan Armağan Uysal , Turan Poyraz , Zekiye Altun , Derya Kaya Pınar Özçelik , Egemen İdiman

Abstract

Objective: Multiple sclerosis (MS) is a heterogeneous disease with clinical and immunological features. Most MS cases occursporadically, but a considerable proportion of patients have a family history of MS. The etiology and pathophysiology of MSremainunclear. Recent epidemiological and gene expression studies have indicated that dysregulation of microRNAs (miRNAs) may playarole in MS pathogenesis. This study aimed to evaluate the differential expression of miRNAs in sporadic MS (sMS) and familial MS(FMS) patients.
Materials and Methods: This cross-section, single-center study was conducted in 20 FMS and 10 sMS patients and 8 healthycontrols. The patients were in the remission. In total, 2,549 miRNA genes were screened in the blood mononuclear cells fromthewhole blood samples of MS patients depending on miRBase 21. Differential expression of miRNAs in MS patients was identifiedcompared with the control group, and miRNAs with a fold change ≥2 were validated using reverse transcription-polymerase chainreaction. Differentially expressed miRNAs were then compared between FMS and sMS patients.
Results: Initial findings showed that miR-5100 and hsa-miR-16-2-3p were increased and miR-432-3p was decreased inFMScompared with sMS, whereas miR-548-aa, hsa-miR-142-3p, and miR-451-b were increased in both sMS and FMS, but miR-548-vwas increased only in sMS. Some miRNAs showed the same expression patterns in both groups.
Conclusion: Differential expression of certain miRNAs may be a useful biomarker in the diagnosis of MS. This study showedthat miRNAs may discriminate between FMS and sMS cases and MS subtypes, as indicated in earlier studies.

Keywords

Multiple sclerosis, familial MS, sporadic MS, miRNA expression

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