The Effect of Niacin and Melatonin Supplementation on the Antioxidant System and Lipid Peroxidation in Exercised Rats

Year 2022, Volume: 23 Issue: 1, 60 - 66, 30.03.2022

Adem Keskin , Aslıhan Büyüköztürk Karul

Abstract

Objective: The aim of this study was to examine the effect of niacin and melatonin supplementation on the antioxidant system and lipid peroxidation in exercised rats.
Materials and Methods: The rats were divided into four groups. Running exercise was done for ten days. Niacin and melatonin were given before exercise.
Results: The malondialdehyde levels of the melatonin group were lower than those of the other groups. Malondialdehyde levels of the niacin + melatonin group were higher than the control groups super oxide dismutase (SOD) levels of the melatonin group were higher than those of the other groups. SOD levels of the niacin + melatonin group were lower than those of the other groups. Catalase levels of the melatonin group were higher than those of the niacin groups. Glutathione peroxidase levels of the control group were lower than those of the other groups. Total oxidant levels of the control group were lower than those of the niacin groups. Total antioxidant level (TAS) levels of the control group were lower than those of the other groups. TAS levels of the niacin + melatonin group were lower than those of the niacin or melatonin groups. Melatonin levels of the melatonin groups were higher than those of the other groups.
Conclusion: Melatonin supplementation decreased malondialdehyde levels and increased antioxidant parameters. It can be said that it has a higher antioxidant effect compared to niacin. Co-administration of niacin and melatonin increased oxidation parameters and decreased SOD and catalase levels. These enzymes indicated that they were used in response to increased oxidant status.

Keywords

Exercise , melatonin , niacin , lipid peroxidation , antioxidant

References

• 1. Górnicka M, Drywień M, Frąckiewicz J, Dębski B, Wawrzyniak A. Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms. Adv Clin Exp Med 2016; 25: 673-9.
• 2. Pialoux V, Mounier R, Ponsot E, Rock E, Mazur A, Dufour S, et al. Effects of exercise and training in hypoxia on antioxidant/prooxidant balance. Eur J Clin Nutr 2006; 60: 1345-54.
• 3. Granger DN, Kvietys PR. Reperfusion injury and reactive oxygen species: The evolution of a concept. Redox Biol 2015; 6: 524-51.
• 4. Alonso M, Collado PS, González-Gallego J. Melatonin inhibits the expression of the inducible isoform of nitric oxide synthase and nuclear factor kappa B activation in rat skeletal muscle. J Pineal Res 2006; 41: 8-14.
• 5. Brzezinski A. Melatonin in humans. N Engl J Med 1997; 336: 186-95.
• 6. Ağırbaş Ö. Effects of Melatonin and Ascorbic Acid on DNA Damage and Oxidative Stress Emerging in Muscles in Rats on Which Intensive Exercise Stress Was Created. Erzurum. Atatürk University. 2013.
• 7. Burtis CA, Ashwood ER. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics 5th Ed. 2010.
• 8. Ilkhani F, Hosseini B, Saedisommeolia A. Niacin and Oxidative Stress: A Mini-Review. J Nutri Med Diet Care 2016; 2: 1-6.
• 9. Pingitore A, Lima GP, Mastorci F, Quinones A, Iervasi G, Vassalle C. Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports. Nutrition 2015; 31: 916-22.
• 10. Aguiló A, Tauler P, Pilar Guix M, Villa G, Córdova A, Tur JA, et al. Effect of exercise intensity and training on antioxidants and cholesterol profile in cyclists. J Nutr Biochem 2003; 14: 319-25.
• 11. Ziaadini F, Aminae M, MahsaRastegar MM, Abbasian S, Memari AH. Melatonin Supplementation Decreases Aerobic Exercise Training Induced-Lipit Peroxidation and Malondialdehyde in Sedentary Young Women. Pol J Food Nutr Sci 2017; 67: 225-32.
• 12. Mohn A, Catino M, Capanna R, Giannini C, Marcovecchio M, Chiarelli F. Increased oxidative stress in prepubertal severely obese children: effect of a dietary restriction-weight loss program. J Clin Endocrinol Metab 2005; 90: 2653-8.
• 13. Cantó C, Houtkooper RH, Pirinen E, Youn DY, Oosterveer MH, Cen Y, et al. The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. Cell Metab 2012; 15: 838-47.
• 14. Beck WR, Botezelli JD, Pauli JR, Ropelle ER, Gobatto CA. Melatonin Has An Ergogenic Effect But Does Not Prevent Inflammation and Damage In Exhaustive Exercise. Sci Rep 2015; 5: 18065.
• 15. Yang Y, Wu T, He K, Fu ZG. Effect of aerobic exercise and ginsenosides on lipid metabolism in diet-induced hyperlipidemia mice. Zhongguo Yao Li Xue Bao 1999; 20: 563-5.
• 16. Aydemir T, Kuru K. Purification and partial characterization of catalase from chicken erythrocytes and the effect of various inhibitors on enzyme activity. Turkish Journal of Chemistry 2003; 27: 85-97.
• 17. Kumar KV, Mur N. Effect Of Oral Melatonın On Exercıse-Induced Oxıdant Stress In Healthy Subjects. Indian Journal of Pharmacology 2002; 34: 256-9.
• 18. Ortiz-Franco M, Planells E, Quintero B, Acuña-Castroviejo D, Rusanova I, Escames G, et al. Effect of Melatonin Supplementation on Antioxidant Status and DNA Damage in High Intensity Trained Athletes. Int J Sports Med 2017; 38: 1117-25.
• 19. Ganji SH, Kashyap ML, Kamanna VS. Niacin inhibits fat accumulation, oxidative stress, and inflammatory cytokine IL-8 in cultured hepatocytes: Impact on non-alcoholic fatty liver disease. Metabolism 2015; 64: 982-90.
• 20. Stacchiotti A, Favero G, Rodella LF. Impact of Melatonin on Skeletal Muscle and Exercise. Cells 2020; 9: 288.
• 21. Escames G, Ozturk G, Baño-Otálora B, Pozo MJ, Madrid JA, Reiter RJ, et al. Exercise and melatonin in humans: reciprocal benefits. J Pineal Res 2012; 52: 1-11.
• 22. Lopez-Flores M, Luque-Nieto R, Moreira OC, Suarez-Iglasias D, Villa-Vicente JG. Effects of Melatonin on Sports Performance: A Systematic Review. Journal of Excise Physiology online. 2018; 21: 121-38.
• 23. Favero G, Stacchiotti A, Castrezzati S, Bonomini F, Albanese M, Rezzani R, et al. Melatonin reduces obesity and restores adipokine patterns and metabolism in obese (ob/ob) mice. Nutr Res 2015; 35: 891-900.
• 24. Salucci S, Battistelli M, Baldassarri V, Burini D, Falcieri E, Burattini S. Melatonin prevents mitochondrial dysfunctions and death in differentiated skeletal muscle cells. Microsc Res Tech 2017; 80: 1174-81.
• 25. Sayed RKA, Fernández-Ortiz M, Diaz-Casado ME, Aranda-Martínez P, Fernández-Martínez J, Guerra-Librero A, et al. Lack of NLRP3 Inflammasome Activation Reduces Age-Dependent Sarcopenia and Mitochondrial Dysfunction, Favoring the Prophylactic Effect of Melatonin. J Gerontol A Biol Sci Med Sci 2019; 74: 1699-708.