Evaluation of the Anti-cancer and Biological Effects of Boric Acid on Colon Cancer Cell Line

Year 2020, Volume: 21 Issue: 3, 238 - 243, 31.12.2020

Ayşe Çiğel Mehmet Dinçer Bilgin , Rauf Onur Ek

https://doi.org/10.4274/meandros.galenos.2020.82246

Cited By: 6

Abstract

Objective: The role of boron in biology includes the coordinated regulation of gene expression as well as the growth and proliferation of all living organisms. Boric acid is known to regulate the proliferation of cancer cells. Colon cancer is among the types of cancer with a high rate of mortality in both men and women. The aim of this study is to evaluate the effects of boric acid (the dominant of boron in plasma) on Caco-2 colorectal cancer cell line.
Materials and Methods: Firstly, the cytotoxic effect of boric acid (0 to 1 mM) on Caco-2 human colon cancer cells was determined and the expressions of Caspase-3 and BCL-2 were evaluated using Western Blot and sodium dodecyl sulphatepolyacrylamide gel electrophoresis analysis.
Results: Our results showed that boric acid inhibits the proliferation Caco-2 cancer cells in a dose dependent manner. We also demonstrated that BCL-2 protein expression decreased with increasing concentrations of boric acid.
Conclusion: This is the first study that demonstrates the anti-cancer effects of boric acid against Caco-2 cancer cell line. Further studies using advanced molecular techniques are needed to precisely explain the anti-cancer mechanisms of boric acid.

Keywords

Boric acid , colon cancer , Caco-2 cell line , Caspase-3 , BCL-2

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