Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis

Year 2018, Volume: 19 Issue: 4, 289 - 295, 31.12.2018

İsmail Özkoçak Hakan Göktürk , Umut Safiye Şay Coşkun Fatma Aytaç

Abstract

Objective: The aim of this study was to evaluate bacterial reduction after using different irrigants, lasers and photodynamic therapy (PDT) in root canals infected by Enterococcus faecalis.
Materials and Methods: Seventy human maxillary incisors were used in this study. After endodontic preparation, sixty teeth were infected by 20 µl E. faecalis (ATCC 29212) and specimens were incubated for 72 hours. Groups were formed according to the irrigation protocol used: group 1: negative control, group 2: positive control, group 3: 10 mL 2% chlorhexidine (CHX), group 4: 10 mL 5% NaOCl, group 5: diode laser, group 6: Er:YAG laser, and group 7: indocyanine green (ICG)-diode laser (PDT). After disinfection procedures the samples isolated from root canals were placed into blood agar, incubated at 37 °C for 24 h and then were counted for colony-forming units. One-Way ANOVA was used for the statistical evaluation of data. For multiple comparisons, Tamhane’s T2 test was used.
Results: All investigated disinfection procedures had a significant reduction in the bacterial population (p<0.001). CHX, NaOCl and ICG-diode laser groups were equally effective in reducing E. faecalis populations (p>0.05) and statistically more effective than both laser groups (p<0.05).
Conclusion: Promising results were obtained by using PDT with ICG. This alternative disinfection method found effective on E. faecalis.

Keywords

Endodontics , Enterococcus faecalis , lasers , photodynamic therapy

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