Molecular detection of feline calicivirus (FCV) in cats with oral lesions

Year 2024, Volume: 9 Issue: 1, 261 - 265, 12.06.2024

Hasbi Sait Saltık , Zehra Erdağı

Abstract

Feline Calicivirus (FCV) is a major cause of oral lesions in cats with respiratory tract disease (RTD). FCV is a single-stranded, positive-polarity RNA virus that encodes three open reading frames (ORFs). Active virus excretion occurs through the saliva of cats infected with FCV, which belongs to the Vesivirus genus of the Caliciviridae family. Oral mucosal lesions caused by infectious agents in RTD lead to significant impairment in the quality of life of cats. RTD, which also affects the oral mucosa, is a common problem in cats. Ten cats of different ages, breeds, and genders with ocular lesions were used in this study. At the time of sample collection, the veterinarian performed general and oral examinations on each animal. On oral examination,varying degrees of gingivitis, stomatitis, and ulceration symptoms were noted. Samples were extracted using a commercial viral nucleic acid isolation kit. Three out of ten samples (30%) were found to be positive for FCV using RT-PCR. T In conclusion, the high sensitivity, specificity, and potential for field sample testing make RT-PCR a very important and inevitable method for research and clinical diagnosis related to FCV infection in cats with oral lesions.

Keywords

calicivirus, feline, oral lesions, respiratory tract disease, RT-PCR

Ethical Statement

All procedures were approved by the Animal Ethics Committee (AEC) Burdur Mehmet Akif University, Türkiye (No:102/915).

Project Number

1919B012112850

Thanks

We gratefully acknowledge the support of TUBITAK, which provided financing for the research.

References

• Abd-Eldaim, M. M., Wilkes, R. P., Thomas, K. V., & Kennedy, M. A. (2009). Development and validation of a TaqMan real-time reverse transcription-PCR for rapid detection of feline calicivirus. Archives of virology, 154, 555-560.
• Aydin, H., & Timurkan, M. O. (2018). A pilot study on feline astrovirus and feline panleukopenia virus in shelter cats in Erzurum, Turkey. Rev. Med. Vet, 169(1/3), 52-57.
• Baydar, E., Eröksüz, Y., Timurkan, M. Ö., & Eröksüz, H. (2014). Feline infectious peritonitis with distinct ocular involvement in a cat in Turkey. 20 (6), 961-965
• Binns, S. H, Dawson, S., Speakman, A. J., Cuevas, L.E., Hart, C. A., Gaskell, C. J., ... & Gaskell, R. M. (2000). A study of feline upper respiratory tract disease with reference to prevalence and risk factors for infection with feline calicivirus and feline herpesvirus. Journal of feline medicine and surgery, 2(3), 123-133.
• Cao, N., Tang, Z., Zhang, X., Li, W., Li, B., Tian, Y., & Xu, D. (2022). Development and application of a Triplex TaqMan quantitative real-time PCR assay for simultaneous detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1. Frontiers in Veterinary Science, 8, 792322.
• Coyne, K. P., Dawson, S., Radford, A. D., Cripps, P. J., Porter, C. J., McCracken, C. M., & Gaskell, R. M. (2006). Long-term analysis of feline calicivirus prevalence and viral shedding patterns in naturally infected colonies of domestic cats. Veterinary microbiology, 118(1-2), 12-25.
• Dağalp, S. B., Doğan, F., Farzanİ, T. A., Babaoğlu, A. R., Kırmızı, G. A., & Çabalar, M. (2019). Molecular investigation of Feline Herpesvirus 1 (FHV-1) and feline calicivirus in cats with respiratory system problem. Eurasian Journal of Veterinary Sciences, 2019, Vol. 35, No. 3, 131-138. https://doi.org/10.15312/EurasianJVetSci.2019.236
• Druet, I., & Hennet, P. (2017). Relationship between Feline calicivirus Load, Oral Lesions, and Outcome in Feline Chronic Gingivostomatitis (Caudal Stomatitis): Retrospective Study in 104 Cats. Frontiers in Veterinary Science, 2017; 4: 209.
• Fontes, A. C, Vieira, M. C., Oliveira, M., Lourenço, L., Viegas, C., Faísca, P., Seixas, F., Requicha, J. F., & Pires, M. A. (2023). Feline calicivirus and natural killer cells: A study of its relationship in chronic gingivostomatitis. Veterinary World 16(8): 1708–1713.
• Henzel, A., Brum, M. C. S., Lautert, C., Martins, M., Lovato, L. T., Weiblen, R. (2012). Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology 43, 560-568. https://doi.org/10.1590/S1517-83822012000200017
• Hofmann-Lehmann, R., Hosie, M. J., Hartmann, K., Egberink, H., Truyen, U., Tasker, S., ... & Möstl, K. (2022). Calicivirus infection in cats. Viruses, 14(5), 937.
• Karapinar, Z., & Timurkan, M.O. (2024). Heterogeneity of Feline Parvoviruses Genotypes and Determination of Distinct Genetic Lineages in Circulation in Turkey. Pakistan Journal of Zoology, 56(2).587-594
• Kim, S. J., Park, Y. H., & Park, K. T. (2020). Development of a novel reverse transcription PCR and its application to field sample testing for feline calicivirus prevalence in healthy stray cats in Korea. Journal of Veterinary Science, 21(5).
• Knowles, J. O., Gaskell, R. M., Gaskell, C. J., Harvey, C.E., & Lutz, H. (1989). Prevalence of feline calicivirus, feline leukaemia virus and antibodies to FIV in cats with chronic stomatitis. The Veterinary Record, 124(13), 336-338.
• Koc, B. T. & Oguzoglu, T. C. (2020). A phylogenetic study of Feline Immunodeficiency Virus (FIV) among domestic cats in Turkey. Comparative Immunology, Microbiology and Infectious Diseases, Dec:73:101544. https://doi.org/10.1016/j.cimid.2020.101544
• Lee, Y., Maes, R., Tai, S. H. S, & Hussey, G. S. (2019). Viral replication and innate immunity of feline herpesvirus-1 virulence-associated genes in feline respiratory epithelial cells. Virus Research 264, 56-67. https://doi.org/10.1016/j.virusres.2019.02.013
• Lommer, M. J., & Verstraete, F. J. M. (2003). Concurrent oral shedding of feline calicivirus and feline herpesvirus 1 in cats with chronic gingivostomatitis. Oral microbiology and immunology, 18(2), 131-134.
• Mochizuki, M., Kawakami, K., Hashimoto, M. & Ishida, T., (2000). Recent epidemiological status of feline upper respiratory infections in Japan. Journal of Veterinary Medical Science, 62, 801-803
• Ohe, K., Sakai, S., Sunaga, F., Murakami, M., Kiuchi, A., Fukuyama, M., ... & Taneno A (2006). Detection of feline calicivirus (FCV) from vaccinated cats and phylogenetic analysis of its capsid genes. Veterinary research communications, 30(3), 293-305.
• Oskarsson, K., Axelsson, P. L., & Penell, J. C. (2021). Dental Problems and Prophylactic Care in Cats—Knowledge and Perceptions among Swedish Cat Owners and Communication by Veterinary Care Staff. Animals, 11(9), 2571.
• Palombieri, A., Sarchese, V., Giordano, M. V., Fruci, P., Crisi, P. E., Aste, G., ... & Di Profio, F. (2022). Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease. Animals, 13(1), 65.
• Pedersen, N. C., Sato, R., Foley, J. E., & Poland, A. M. (2004). Common virus infections in cats, before and after being placed in shelters, with emphasis on feline enteric coronavirus. Journal of feline medicine and surgery, 6(2), 83-88.
• Radford, A. D., Coyne, K. P., Dawson, S., Porter, C. J., & Gaskell, R. M. (2007). Feline calicivirus. Veterinary Research, 2007, 38 (2), pp.319-335.
• Radford, A., Maria A., & Sykes, J. E. (2021). Feline Calicivirus Infections. In: J. E. Sykes (Ed.). Greene’s Infectious Diseases of the Dog and Cat (pp. 443-454). WB Saunders.
• Schulz, C., Hartmann, K., Mueller, R. S., Helps, C., & Schulz, B. S. (2015). Sampling sites for detection of feline herpesvirus-1, feline calicivirus and Chlamydia felis in cats with feline upper respiratory tract disease. Journal of feline medicine and surgery 17(12), 1012-1019.
• Sykes, J. E., Browning, G. F., Anderson, G., Studdert, V. P., & Smith, H. V. (1997). Differential sensitivity of culture and the polymerase chain reaction for detection of feline herpesvirus 1 in vaccinated and unvaccinated cats. Arch Virol 142(1), 65-74. https://doi.org/10.1007/s007050050059.
• Walter J, Foley P, Yason C, Vanderstichel R, & Muckle A. (2020). Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydia felis, and Bordetella bronchiseptica in a population of shelter cats on Prince Edward Island. Can J Vet Res 84(3), 181-188.
• Weeks, M. L., Gallagher, A., & Romero, C. H. (2001) Sequence analysis of feline caliciviruses isolated from the oral cavity of clinically normal domestic cats (Felis catus) in Florida. Research in Veterinary Science 71:223–225.
• Westman, M. E., Coggins, S. J., van Dorsselaer, M., Norris, J. M., Squires, R. A., Thompson, M., & Malik, R. (2022). Feline immunodeficiency virus (FIV) infection in domestic pet cats in Australia and New Zealand: Guidelines for diagnosis, prevention and management. Australian Veterinary Journal. Aug; 100(8): 345–359. https://doi.org/10.1111/avj.13166
• Zheng, M., Li, Z., Fu, X., Lv, Q., Yang, Y., & Shi, F. (2021). Prevalence of feline calicivirus and the distribution of serum neutralizing antibody against isolate strains in cats of Hangzhou, China. Journal of Veterinary Science, 22(5).