Investigation of the shape of goat (capra hircus) astragalus via geometric morphometry method

Year 2024, Volume: 9 Issue: 2, 277 - 282, 31.08.2024

Tuğba Oktay , Yasin Demiraslan , Özcan Özgel

https://doi.org/10.24880/meditvetj.1541126

Abstract

The aim of this study is to determine the effect of sex on shape of goat astragalus via geometric morphometry method. A total of 37 astragalus bone samples collected from 16 female and 21 male goats were used as material. Bone samples were cleaned from skin and soft tissue and then macerated by boiling. Astragalus bone samples were photographed from a distance of 20 cm dorsally by focusing on the center of the bone. The photographs were transferred to the computer in JPEG format. Principal Component Analysis, Regression Analysis, Canonical Variate Analysis, and Discriminant Function Analysis were conducted using the Cartesian coordinate values, which were obtained by homologous landmark marking. The first two principal components accounted for 75.967% of the total shape variation. Shape variation was determined in different regions of the astragalus. According to the scatter plot of male and female individuals, male individuals were completely placed within the confidence interval ellipse of female individuals. It was found that allometric effect on the shape of astragalus bone was not statistically significant. As a result of Canonical Variate Analysis, mahalanobis and procrustes distances were detected as 2.9216 (p<0.0001) and 0.0645 (p=0.0035), respectively. This test indicated two female individuals in the group of males. The proximal of male goat astragalus was wider than that of female goats. The results of the Discriminate Function Analysis revealed that 8 of the female individuals and 7 of the male individuals were incorrectly grouped according to the cross validation scores. Geometric morphometry and the related analyses allowed to examine the differences between the astragalus bone samples of male and female goats. The fact that the astragalus bone of male goats was wider than that of females supported the studies using linear measurements in the literature. Consequently, the result indicating that sex factor had a limited grouping effect on astragalus shape in goats was obtained.

Keywords

astragalus, capra hircus, gender shape

Ethical Statement

According to Article 8 of the Regulation on Working Procedures and Principles of Animal Experiments Ethics Committees dated 15 February 2014 and numbered 28914, Ethics Committee Approval is not required (E-93773921-020-354464).

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