Streptozotosin İndüklenen Alzheimer Hastalığı Modelinde Nörogenez ve Metabolik Parametreler Arasındaki İlişkinin Araştırılması

Abstract

Amaç: Bu çalışmada, STZ kaynaklı Alzheimer hastalığı modelinde nöroplastisite ile ilişkili proteinler (DCX, EGR1, sFRP3) ile metabolik belirteçler (insülin, HbA1c, asprosin) arasındaki etkileşimleri analiz etmeyi ve bu parametrelerdeki değişikliklerin Alzheimer hastalığının (AD) patofizyolojisini nasıl etkilediğini değerlendirmeyi amaçladık. Yöntemler: 8 ila 10 haftalık, 250-300 g ağırlığında 14 erkek Wistar albino sıçan kullanıldı. Hayvanlar iki gruba ayrıldı: kontrol ve Alzheimer modeli. AD modeli, STZ'nin stereotaksik enjeksiyonuyla oluşturuldu ve kontrol grubuna yapay BOS enjekte edildi. Hayvanlar ameliyattan sonra 14 gün boyunca izlendi. Çalışmanın sonunda serum örnekleri toplandı ve DCX, EGR1, sFRP3, insülin, gHbA1c ve asprosin düzeyleri ELISA ile analiz edildi. Değişkenler gruplar arasında karşılaştırıldı ve P değerleri < 0,05 anlamlı kabul edildi. Sonuçlar: DCX ve EGR1 düzeyleri istatistiksel anlamlılık olmaksızın sınırda değişiklikler gösterdi (sırasıyla p = 0,056 ve p = 0,080), sFRP3 düzeyleri ise AD grubunda anlamlı olarak daha yüksekti (p = 0,012). İnsülin düzeyleri farklılık göstermedi (p = 0,829), ancak asprosin ve gHbA1c düzeyleri anlamlı olarak daha yüksekti (sırasıyla p = 0,046 ve p = 0,000), bu da metabolik düzensizliği gösteriyordu. Korelasyon analizi asprosin ve gHbA1c (r = 0,807, p < 0,01) ve sFRP3 ve EGR1 (r = 0,780, p < 0,01) arasında güçlü pozitif korelasyonlar gösterdi, bu da AD patofizyolojisinde birbirine bağlı roller olduğunu düşündürmektedir. Sonuç: Bu çalışma AD'de nörogenez ve metabolik parametreler arasındaki ilişkiyi göstermektedir.

Keywords

• Alzheimer Hastalığı
• Streptozotosin
• Nörogenez
• Metabolik Parametreler
• İnsülin Direnci

Investigation of the Relationship Between Neurogenesis and Metabolic Parameters in the Streptozotocin-Induced Alzheimer’s Disease Model

Abstract

Aim: In this study, we aimed to analyze the interactions between neuroplasticity-related proteins (DCX, EGR1, sFRP3) and metabolic markers (insulin, HbA1c, asprosin) in an STZ-induced Alzheimer's disease model, as well as to evaluate how changes in these parameters affect the pathophysiology of Alzheimer's disease (AD). Material and Methods: 14 male Wistar albino rats, aged 8 to 10 weeks, weighing 250–300 g, were used. The animals were divided into two groups: control and Alzheimer's model. The AD model was created by stereotaxic injection of STZ, and the control group was injected with artificial CSF. The animals were monitored for 14 days after surgery. At the end of the study, serum samples were collected and DCX, EGR1, sFRP3, insulin, gHbA1c, and asprosin levels were analyzed by ELISA. The variables were compared between the groups, and P-values < 0.05 were considered significant. Results: DCX and EGR1 levels showed borderline changes without statistical significance (p = 0.056 and p = 0.080, respectively), while sFRP3 levels were significantly higher in the AD group (p = 0.012). Insulin levels did not differ (p = 0.829), but asprosin and gHbA1c levels were significantly higher (p = 0.046 and p = 0.000, respectively), indicating metabolic derangement. Correlation analysis showed strong positive correlations between asprosin and gHbA1c (r = 0.807, p < 0.01) and sFRP3 and EGR1 (r = 0.780, p < 0.01), suggesting interconnected roles in AD pathophysiology. Conclusion: This study shows the relationship between neurogenesis and metabolic parameters in AD.

Keywords

• Alzheimer
• Streptozotocin
• Neurogenesis
• Metabolic Parameters
• Insulin Resistance

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