Bilateral Arteria Carotis Communis Ligasyonu Sonrası Arteria Basilaris’te Gözüken Morfohistopatolojik Değişikliklere Cinsiyetin Etkisi: Deneysel Çalışma

Year 2020, Volume: 2 Issue: 2, 28 - 33, 28.07.2020

Cengiz Öztürk , Kemal Alp Nalcı , Onur Ceylan , İsmail Malkoç , Mehmet Aydin

https://doi.org/10.37990/medr.760505

Abstract

incelmesi, uzama, kıvrılma, vasküler remodelizasyon ve anevrizmalara sebep olabilir. Biz cinsiyetin bilateral arteria carotis communis
ligasyonu sonucu oluşan histomorfolojik değişikliklerin derecesine olan etkisini araştırdık.
Materyal ve Metot: Bu çalışma 7 dişi ve 7 erkek tavşan üzerinde yapılmıştır. Hem erkek hem de dişi tavşanlardan ikisi kontrol, diğerleriyse
deney grubu olarak kullanıldı. Arteria carotis communis bifurkasyonunun hemen proksimalinden uygulanan kalıcı ligasyon, steno-oklüziv
karotis arter hastalığını tekrarlamak için yapıldı. Hayvanlar üzerinde yapılan bu çalışmanın 3. haftasında baziler arter hacmi stereolojik
yöntemlerle ölçüldü. Sonuçlar Mann-Whitney U testi ile karşılaştırıldı.
Bulgular: Baziler arterlerde büyük oranda luminal genişleme, duvar incelmesi, uzaması, kıvrılması ve doligoektatik kümelenme tespit edildi.
Baziler arterlerin ortalama hacmi erkek kontrol grubu tavşanlarında 3,65 ± 0,35 mm3, erkek deney tavşanlarında ise 4.96 ± 0.99 mm3 olarak
değerlendirildi. Baziler arterlerin ortalama hacim değerleri dişi kontrol grubu tavşanlarında 3,97 ± 0,40 mm3, dişi deney tavşanlarında ise
6,32 ± 1,13 mm3 olduğu tespit edildi. Baziler arter genişlemesi dişi tavşanlarda erkeklerden gözle görülür derecede belirgindi (p<0.01).
Sonuç: Bilateral arteria carotis communis ligasyonu, dişi tavşanlarda erkek tavşanlara göre daha belirgin luminal genişleme, duvar
incelmesi, uzama, kıvrılma ve anevrizmalara sebep oluyor.

Keywords

Bilateral arteria carotis communis ligasyonu, baziler arterler, stereolojik yöntem

Effect of Gender on Morphohistopathological changes of Basilar Arteries Following Bilateral Common Carotid artery Ligation: An Experimental Study

Abstract

Aim: Steno-occlusive carotid artery disease causes increased pressure, retrograde blood flow, luminal enlargement, vascular wall
thinning, elongation, convolutions, vascular re-modelization, and aneurysm formation in basilar arteries. We investigated if gender affects
the severity of histomorphologic alterations of basilar arteries after bilateral common carotid artery ligation.
Material and Methods: This study was conducted on 7 male and 7 female rabbits. Two of both male and female rabbits were used as a
control group and the remaining were used as the study group. Permanent ligation of the common carotid arteries from just proximal of
bifurcation was performed to replicate steno-occlusive carotid artery disease. Basilar artery volumes were measured by stereological
methods after the sacrifice of the animals at the end of the 3rd week. Results were compared Mann-Witney U test between groups.
Results: Luminal enlargement, wall thinning, elongation, convolutions, and doligoectatic configurations were detected in the majority of
basilar arteries. The mean basilar arterial volume values were estimated as male rabbits were 3.65 ± 0.35 mm3 in control and 4.96 ± 0.99
mm3 in the study group. The mean basilar arterial volume values were estimated as female rabbits were 3.97 ± 0.40 mm3 in control and
6.32 ± 1.13 mm3 in the study group. The severity of basilar enlargement was significantly higher in the female rabbits compared with the
male rabbits (p < 0.01).
Conclusion: Bilateral common carotid artery ligation causes more severe luminal enlargement, wall thinning, elongation, convolution, and
aneurysm formation in female rabbits than males.
Keywords: Bilateral common carotid artery ligation (BCCAL), basilar artery (BA), stereological method

Keywords

common carotid ligation,, basilar arteries, stereological method

References

• Aygul R, Aydin MD, Kotan D, Demir R, Ulvi H, Karalar M, Nalbantoglu NG, Eseoglu M. Role of the trigeminal system on posterior communicating artery remodelization after bilateral common carotid artery ligation. Anal Quant Cytopathol Histpathol. 2013 Aug;35(4):217-25.
• Castelló-Ruiz M, Salom JB, Fernández-Musoles R, Burguete MC, López-Morales MA, Arduini A, Jover-Mengual T, Hervás D, Torregrosa G, Alborch E. Relaxant Effects of the Selective Estrogen Receptor Modulator, Bazedoxifene, and Estrogen Receptor Agonists in Isolated Rabbit Basilar Artery. J Cardiovasc Pharmacol. 2016 Oct;68(4):313-321.
• Chang CM, Su YF, Chang CZ, Chung CL, Tsai YJ, Loh JK, Lin CL. Pgesterone attenuates experimental subarachnoid hemorrhage-induced vasospasm by upregulation of endothelial nitric oxide synthase via Akt signaling pathway. 1. Biomed Res Int. 2014;2014:207616. doi: 10.1155/2014/207616. Epub 2014 May 13.
• Chrissobolis S, Sobey CG. Influence of gender on K+-induced cerebral vasodilatation. Stroke. 2004 Mar;35(3):747-52. Epub 2004 Feb 5.
• Eseoglu M, Yilmaz I, Karalar M, Aydin MD, Kayaci S, Gundogdu C, Gunaldi O, Onen MR. The role of sympathectomy on the regulation of basilar artery volume changes in stenoocclusive carotid artery modeling after bilateral common carotid artery ligation: an animal model. Acta Neurochir (Wien). 2014 May;156(5):963-9.
• Fredriksson K, Nordborg C, Johansson BB. The hemodynamic effect of bilateral carotid artery ligation and the morphometry of the main communicating circuit in normotensive and spontaneously hypertensive rats. Acta Physiol Scand. 1984 Jul;121(3):241-7.
• Futo J, Shay J, Block S, Holt J, Beach M, Moss J. Estrogen and progesterone withdrawal increases cerebral vasoreactivity to serotonin in rabbit basilar artery. Life Sci. 1992;50(16):1165-72.
• Gao L, Hoi Y, Swartz DD, Kolega J, Siddiqui A, Meng H. Nascent aneurysm formation at the basilar terminus induced by hemodynamics. Stroke. 2008 Jul;39(7):2085-90.
• Gürer B, Turkoglu E, Kertmen H, Karavelioglu E, Arikok AT, Sekerci Z. Attenuation of cerebral vasospasm and secondary injury by testosterone following experimental subarachnoid hemorrhage in rabbit. Acta Neurochir (Wien). 2014 Nov;156(11):2111-20; discussion 2120. doi: 10.1007/s00701-014-2211-9. Epub 2014 Sep 7.
• Hoi Y, Gao L, Tremmel M, Paluch RA, Siddiqui AH, Meng H, Mocco J. In vivo assessment of rapid cerebrovascular morphological adaptation following acute blood flow increase. J Neurosurg. 2008 Dec;109(6):1141-7.
• Kilic M, Kilic B, Aydin MD, Kanat A, Yilmaz I, Eseoglu M, Gundogdu B. Paradoxic Relations between Basilar Artery Reconfiguration and Superior Cervical Ganglia Ischemia After Bilateral Common Carotid Artery Ligation. World Neurosurg. 2019 May;125:e658-e664.
• Kim SH, Kim EH, Lee BI, Heo JH. Chronic cerebral hypoperfusion protects against acute focal ischemia, improves motor function, and results in vascular remodeling. Curr Neurovasc Res. 2008 Feb;5(1):28-36.
• Lehman RM, Owens GK, Kassell NF, Hongo K. Mechanism of enlargement of major cerebral collateral arteries in rabbits. Stroke. 1991 Apr;22(4):499-504.
• Mandelbaum M, Kolega J, Dolan JM, Siddiqui AH, Meng H.A critical role for proinflammatory behavior of smooth muscle cells in hemodynamic initiation of intracranial aneurysm. PLoS One. 2013 Sep 2;8(9):e74357.
• Masuda H, Sugita A, Zhuang YJ. Pathology of the arteries in the central nervous system with special reference to their dilatation: blood flow. Neuropathology. 2000 Mar;20(1):98-103.
• Meng H, Metaxa E, Gao L, Liaw N, Natarajan SK, Swartz DD, Siddiqui AH, Kolega J, Mocco J Progressive aneurysm development following the hemodynamic insult. J Neurosurg. 2011 Apr;114(4):1095-103.
• Metaxa E, Tremmel M, Natarajan SK, Xiang J, Paluch RA, Mandelbaum M, Siddiqui AH, Kolega J, Mocco J, Meng H.Characterization of critical hemodynamics contributing to aneurysmal remodeling at the basilar terminus in a rabbit model. Stroke. 2010 Aug;41(8):1774-82.
• Miller AA, Drummond GR, Mast AE, Schmidt HH, Sobey CG. Effect of gender on NADPH-oxidase activity, expression, and function in the cerebral circulation: role of estrogen. Stroke. 2007 Jul;38(7):2142-9. Epub 2007 May 24.
• Oldendorf WH. Trophic changes in the arteries at the base of the rat brain in response to bilateral common carotid ligation. J Neuropathol Exp Neurol. 1989 Sep;48(5):534-47.
• Ramírez-Rosas MB, Cobos-Puc LE, Muñoz-Islas E, González-Hernández A, Sánchez-López A, Villalón CM, Maassenvandenbrink A, Centurión D. Pharmacological evidence that Ca²+ channels and, to a lesser extent, K+ channels mediate the relaxation of testosterone in the canine basilar artery. Steroids. 2011 Mar;76(4):409-15. doi: 10.1016/j.steroids.2010.12.012. Epub 2010 Dec 28.
• Rots ML, de Borst GJ, van der Toorn A, Moll FL, Pennekamp CWA, Dijkhuizen RM, Bleys R. Effect of bilateral carotid occlusion on cerebral hemodynamics and perivascular innervation: An experimental rat model. J Comp Neurol. 2019 Oct 1;527(14):2263-2272.
• Songsaeng D, Srivatanakul K, Krings T, Geibprasert S, Ozanne A, Lasjaunias P. Symptomatic spontaneous vertebrobasilar dissections in children: a review of 29 consecutive cases. J Neurosurg Pediatr. 2010 Sep;6(3):233-43. doi: 10.3171/2010.6.PEDS09290.
• Tutino VM, Liaw N, Spernyak JA, Ionita CN, Siddiqui AH, Kolega J, Meng H(1). Assessment of Vascular Geometry for Bilateral Carotid Artery Ligation to Induce Early Basilar Terminus Aneurysmal Remodeling in Rats. Curr Neurovasc Res. 2016;13(1):82-92.
• Tutino VM, Mandelbaum M, Choi H, Pope LC, Siddiqui A, Kolega J, Meng H. Aneurysmal remodeling in the circle of Willis after carotid occlusion in an experimental model. J Cereb Blood Flow Metab. 2014 Mar;34(3):415-24.
• Tütüncü F, Schimansky S, Baharoglu M, Gao B, Calnan D, Hippelheuser J, Safain MG, Lauric A, Malek AM. Widening of the basilar bifurcation angle: association with presence of intracranial aneurysm, age, and female sex. J Neurosurg. 2014 Dec;121(6):1401-10.
• Yang YM, Feng X, Yao ZW, Tang WJ, Liu HQ, Zhang L. Magnetic resonance angiography of carotid and cerebral arterial occlusion in rats using a clinical scanner.J Neurosci Methods. 2008 Jan 30;167(2):176-83.
• Yilmaz I, Eseoglu M, Onen MR, Tanrıverdi O, Kilic M, Yilmaz A(1), Musluman AM, Aydin MD, Gündogdu C. Inverse Association Between Basilar Artery Volume and Neuron Density in the Stellate Ganglion Following Bilateral Common Carotid Artery Ligation: An Experimental Study. World Neurosurg. 2017 Apr;100:138-143.