Expression of VEGF in Fallopian tubes in Ovarian Ischemia-Reperfusion

Year 2023, Volume: 5 Issue: 2, 337 - 41, 15.05.2023

Veysel Toprak , Engin Deveci

https://doi.org/10.37990/medr.1240419

Abstract

Aim: Our aim was to investigate expression level of VEGF in tuba uterine in ovarian ischemia-reperfusion (I/R) by immunohistochemical techniques.
Material and Methods: 30 Sprague Dawley female rats were categorized into three groups. Sham group: The abdomen was opened and closed without any treatment. Ischemia (I) group: 1-hour ischemia was allowed to create ischemic injury. Ischemia-reperfusion (I/R) group: 1-hour ischemia and then 3-hour reperfusion was allowed to create I/R injury.
Results: MDA and MPO levels were increased after ischemia and IR while GSH content was decreased. Histological scores of follicular degeneration, inflammation, hemorrhage were high in I and IR groups. Normal histology of tuba uterine was observed in sham group. In I and IR group, degenerated cilia, desquamative epithelial cells impaired basement membrane leukocytes infiltration, apoptotic nuclei, vascular dilatation, thrombosis and inflammation and adenoma were observed. VEGF expression was mainly in sham group. In I and IR group, endothelial cells, adenoma structures, vessels, macrophage and inflammatory leukocyte cells and fibroblast cells showed positive VEGF expression.
Conclusion: IR damage affected inflammation and angiogenesis, changes in implantation.

Keywords

VEGF , ischemia-reperfusion , fallopian tubes , histopathology

References

• Soares ROS, Losada DM, Jordani MC, et al. Ischemia/Reperfusion Injury Revisited: An Overview of the Latest Pharmacological Strategies. Int J Mol Sci. 2019;20:5034.
• Durgun C, Aşir F. Effect of ellagic acid on damage caused by hepatic ischemia reperfusion in rats. Eur Rev Med Pharmacol Sci. 2022;26:8209-15.
• Firat A, Deveci E, Ozkorkmaz EG, et al. The Effect of Momordica charantia Ovarian Ischemia-Reperfusion. Journal of Biosciences and Medicines, 2021;912:8-14.
• Topçu G, Ay M, Bilici A, et al. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chemistry 2007;103:816-22.
• Briceag I, Costache A, Purcarea VL, et al. Fallopian tubes--literature review of anatomy and etiology in female infertility. J Med Life. 2015;8:129-31.
• Lowi RN. Uterine tube physiology. A review of the literature. Obstet Gynecol. 1960;16:322-6.
• Sebastian JA, Baker RL, Cordray D. Asymptomatic infarction and separation of ovary and distal uterine tube. Obstet Gynecol. 1973;41:531-5.
• Kubtan MA. Ovarian and uterine tube infarction in a two years old toddler, case report. MOJ Surg. 2017;4:91-3
• Draper, HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990;186:421-31.
• Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70:158-69.
• Hillegass LM, Griswold DE, Brickson B, Albrightson-Winslow C. Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods. 1990;24:285-95.
• Aktaş A, Aşır F, Başaran SÖ, et al. Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury. J Drug Deliv Ther. 2022;12:26-30.
• Peker N, Deger U, Asır F. Investigation of the effects of carvacrol on experimental ischemia/reperfusion model of rat ovaries by immunohistochemistry. Anal Quant Cytopathol Histopathol. 2021;42:197-204.
• Eser A, Hizli D, Haltas H, et al. Effects of curcumin on ovarian ischemia-reperfusion injury in a rat model. Biomed Rep. 2015;3:807-13.
• Melincovici CS, Boşca AB, Şuşman S, et al. Vascular endothelial growth factor (VEGF) - key factor in normal and pathological angiogenesis. Rom J Morphol Embryol. 2018;59:455-67.
• Ersoy Canillioglu Y, Erkanli Senturk G. Alterations of IL-1 and VEGF After Ischemia-Reperfusion Injured Uterus and Ovary in Rats. Medeni Med J. 2020;35:106-15.
• Deger U, Cavus Y. Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models. Acta Cirúrgica Brasileira. 2020;35.
• Parlakgumus HA, Aka Bolat F, Bulgan Kilicdag E, et al. Atorvastatin for ovarian torsion: effects on follicle counts, AMH, and VEGF expression. Eur J Obstet Gynecol Reprod Biol. 2014;175:186-90.
• Sakar MN, Oğlak SC. Comparison of the efficacy of letrozole stair-step protocol with clomiphene citrate stair-step protocol in the management of clomiphene citrate-resistant polycystic ovary syndrome patients. J Obstet Gynaecol Res. 2021;47:3875-82.
• Oğlak SC, Sakar MN, Ege S, et al. Comparison of the efficacy of letrozole and gonadotropin combination versus gonadotropin alone in intrauterine insemination cycles in patients with unexplained infertility. East J Med. 2020;25:427-33.