Investigation of Aquaporin Molecules in the Placentas of Pregnant Women with Premature Rupture of Membranes

Year 2024, Volume: 6 Issue: 3, 456 - 461, 24.09.2024

Özge Kaplan , Süreyya Özdemir Başaran , Ayşegül Pala , Tuğcan Korak , Fırat Aşır , Serdar Kaplan , Serhat Ege

https://doi.org/10.37990/medr.1517816

Cited By: 3

Abstract

Aim: This study aimed to investigate the immunohistochemical expression of Aquaporin 3 (AQP3) in placentas of pregnant women with premature rupture of membrane (PROM) and to explore AQP3-related interactors and signaling pathways using in silico approaches.
Material and Method: Placental samples from 21 healthy (control) pregnant women and 21 pregnant women diagnosed with PROM were processed for routine histological tissue preparation. Sections were immunostained with AQP3 and analyzed under light microscope via ImageJ software. Protein-protein interaction (PPI) network of AQP3 was constructed with Cytoscape (version 3.10.2). Nodal centrality indexes (degree, closeness and betweenness) were computed through CentiScaPe plugin. The Enrichr tool was utilized to perform pathway enrichment analysis for 15 central genes.
Results: AQP3 immune activity was significantly decreased in the plasma membrane of the trophoblastic cell layer of the PROM group compared to control group. According to network centrality analysis, AQP subfamily proteins predominantly play central roles in the AQP3 network; Major Intrinsic Protein of Lens Fiber (MIP), Glycerol-3-Phosphate Dehydrogenase 2 (GPD2), Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Glycerol Kinase 2 (GK2), GK, and Actin Beta (ACTB) with additional central interactors including proteins. Peroxisome proliferator-activated receptors (PPAR) signaling pathway was obtained as the most significantly enriched pathway.
Conclusion: Alterations in AQP3 expression level in the PROM group compared with the control group may contribute to disturbances in water transport and cellular homeostasis in placental tissues and in silico potential interaction between AQP3 expression and PPAR signaling suggest the role of AQP3 in cell metabolism in PROM.

Keywords

Premature rupture of membranes , aquaporin 3 , placenta , immunohistochemistry

Ethical Statement

Dicle University Faculty of Medicine, non-interventional clinical researches with decision no. 188 on 2023/06.

Thanks

ilginiz için teşekkürler

References

• Meller CH, Carducci ME, Ceriani Cernadas JM, Otaño L. Preterm premature rupture of membranes. Arch Argent Pediatr. 2018;116:e575-81.
• Lin J-H, Hsu Y-H, Wang P-H. Risks for preterm premature labor: many of them are preventable. J Chin Med Assoc. 2020;83:421-2.
• Samejima T, Nagamatsu T, Iriyama T, et al. Impact of additional risk factors on the incidence of preterm delivery among pregnant women diagnosed with short cervix. Taiwan J Obstet Gynecol. 2020;59:195-9.
• Kurek Eken M, Tüten A, Özkaya E, et al. Major determinants of survival and length of stay in the neonatal intensive care unit of newborns from women with premature preterm rupture of membranes. J Matern Fetal Neonatal Med. 2017;30:1972-5.
• Jie C, Mai S, Qiuju L, et al. Investigation of sub-health status of neonates in Heping street region (Beijing) and analysis on related risk factors. Pak J Pharm Sci. 2016;29:2173-7.
• Agre P. The aquaporin water channels. Proc Am Thorac Soc. 2006;3:5-13.
• Zhu X, Jiang S, Zhu X, et al. Expression of aquaporin 1 and aquaporin 3 in fetal membranes and placenta in human term pregnancies with oligohydramnios. Placenta. 2009;30:670-6.
• Damiano A, Zotta E, Goldstein J, et al. Water channel proteins AQP3 and AQP9 are present in syncytiotrophoblast of human term placenta. Placenta. 2001;22:776-81.
• Szpilbarg N, Damiano AE. Expression of aquaporin-3 (AQP3) in placentas from pregnancies complicated by preeclampsia. Placenta. 2017;59:57-60.
• Crowe AR, Yue W. Semi-quantitative determination of protein expression using immunohistochemistry staining and analysis: an integrated protocol. Bio Protoc. 2019;9:e3465.
• Aşır F, Oğlak SC, Ağaçayak E, et al. WIPI-2 protein expression increases in the placentas of patients with preeclampsia. Perinatal Journal. 2023;31:219-23.
• Haznedar B, Kaplan Ö, Aşır F, Ermiş I. Immune activity of Ki-67 during nasal polyp development. Eur Rev Med Pharmacol Sci 2023;27:2759-64.
• Scardoni G, Laudanna C. Centralities based analysis of complex networks. In: Zhang Y, ed. New frontiers in graph theory. Intech, 2012;323-48.
• Xie Z, Bailey A, Kuleshov MV, et al. Gene set knowledge discovery with Enrichr. Curr Protoc. 2021;1:e90.
• Alviggi C, Conforti A, De Rosa P, et al. The distribution of stroma and antral follicles differs between insulin-resistance and hyperandrogenism-related polycystic ovarian syndrome. Front Endocrinol (Lausanne). 2017;8:117.
• Caughey AB, Robinson JN, Norwitz ER. Contemporary diagnosis and management of preterm premature rupture of membranes. Rev Obstet Gynecol. 2008;1:11-22.
• Romero R, Kusanovic JP, Chaiworapongsa T, Hassan S.S, Placental bed disorders in preterm labor, preterm PROM, spontaneous abortion and abruptio placentae. Best Pract Res Clin Obstet Gynaecol. 2011;25:313-27.
• Pérez-Pérez A, Vilariño-García T, Dietrich V, et al. Aquaporins and placenta. Vitam Horm. 2020;112:311-26.
• Ishibashi K, Hara S, Kondo S. Aquaporin water channels in mammals. Clin Exp Nephrol. 2009;13:107-17.
• Seo MJ, Lim JH, Kim DH, Bae HR. Loss of aquaporin-3 in placenta and fetal membranes induces growth restriction in mice. Dev Reprod. 2018;22:263-73.
• Natalia S, Damiano A.E. Expression of aquaporin-3 (AQP3) in placentas from pregnancies complicated by preeclampsia.Placenta. 2017;59:57-60.
• Zhang C, Li Y, Wang J et al. Association between levels of aquaporin 3 in the placenta and adiponectin in the umbilical cord blood with gestational diabetes mellitus and pregnancy outcome. Mol Med Rep. 2020;22:1498-506.
• Wieser F, Waite L, Depoix C, Taylor RN. PPAR action in human placental development and pregnancy and its complications. PPAR Res. 2008;2008:527048.
• Psilopatis I, Vrettou K, Fleckenstein FN, Theocharis S. The role of peroxisome proliferator-activated receptors in preeclampsia. Cells. 2023;12:647.
• Keelan JA, Helliwell RJ, Nijmeijer BE, et al. 15-deoxy-Δ12, 14-prostaglandin J2-induced apoptosis in amnion-like WISH cells. Prostaglandins Other Lipid Mediat. 2001;66:265-82.
• Antoine A, De Sousa Do Outeiro C, Charnay C, et al. Dysregulation of the amniotic PPARγ pathway by phthalates: modulation of the anti-inflammatory activity of PPARγ in human fetal membranes. Life (Basel). 2022;12:544.