Investigation of the Effects of Pendimethalin on Liver and Kidney Tissue in Mice by Histological Methods

Year 2025, Volume: 7 Issue: 1, 263 - 267, 15.01.2025

Yusuf Ersan , Nurdan Tuğba İşçen

https://doi.org/10.37990/medr.1565351

Abstract

Aim: Pesticides, which provide many benefits in terms of production and yield, can also be toxic substances that can harm human health. In this study, the effects of pendimethalin (PND), a type of pesticide, on the liver and kidney tissue of mice were examined by histopathological methods.
Material and Method: A total of 30 mice were used in our study. They were divided into 5 groups, 6 mice in the control group and 6 mice in the other groups. One of the groups was reserved as the control group. No application was made to the control group. Group I received 0.1 mg/l PND, group II received 0.2 mg/l PND intraperitoneally on the 1st and 3rd days of the study. Group III received 0.1 mg/l PND intraperitoneally and 0.1 mg/l vitamin A and C orally, group IV received 0.2 mg/l PND intraperitoneally and 0.1 mg/l vitamin A and C orally on the 1st and 3rd days of our study. On the 4th day of our study, the experimental animals were sacrificed by cervical dislocation method under general anaesthesia. Liver and kidney tissues of the mice were histopathologically examined under light microscope.
Results: In our study, sinusoidal enlargement and vascular congestion were observed in the liver tissue of the I. experimental group, while tubular dilatation and intertubular vascular congestion were observed in the kidney tissue. In the second experimental group, in addition to the similar findings in the first experimental group, an increase in the number of pyknotic nuclei and Kuppfer cells in the liver tissue and loss of cells and disrupted areas in the tubules in the kidney tissue were observed. In addition, the findings in this group were more pronounced. In the third experimental group, histopathological findings were similar to those in the first group. Similarly, the findings in experimental group IV and experimental group II, which were given vitamin A and C, were similar.
Conclusion: In conclusion, our findings showed that PND negatively affected the histology of liver and kidney of mice. Vitamins A and C did not contribute positively to these histopathological findings.

Keywords

Liver tissue , kidney tissue , pendimethalin , histopathology

Ethical Statement

For this study, Saki Yenilli Experimental Animal Production and Application Laboratory Permission for the study was obtained with the decision of the Local Ethics Committee for Animal Experiments (Decision No: 07- 24.04.2019)

Supporting Institution

The study was conducted with project code 2069 at Karabük University Supported by the Scientific Research Projects (BAP) Coordination Unit.

Project Number

The study was conducted with project code 2069 at Karabük University Supported by the Scientific Research Projects (BAP) Coordination Unit.

Thanks

Karabuk University supported the study with project code no. 2069 I would like to thank the Scientific Research Projects (BAP) Coordination Unit.

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