INVESTIGATION OF PLACENTAL HOFBAUER CELLS BY IMMUNOHISTCHEMSTRY METHODS IN COMPLICATED PREGNANCIES

Year 2021, , 150 - 159, 30.12.2021

Yusuf Nergiz , Şebnem Nergiz Öztürk , Fırat Aşır , Ayşe Şahin , Elif Ağaçayak

https://doi.org/10.51477/mejs.1004971

Abstract

Development of placenta without any complication is essential for normal pregnancy. Placenta is a multifunctional organ that plays a vital role in fetal development. Hofbauer cells are one of the most important groups of placental cells. These cells are placental macrophages and have a role in many placental events. The aim of this study is to investigate the placental distribution and density of Hofbauer cells and to contribute to the understanding of the causes and pathogenesis of complicated pregnancies. In this study, 60 full-term placentas were divided into 4 equal groups: control, preeclampsia, gestational diabetes and HELLP group. Placenta were dissected and the samples were fixed 10% neutral buffered formalin. Following routine paraffin wax procedure, 5 µm sections were stained with CD68 for marking Hofbauer cells. In immunohistochemical evaluation, Hofbauer cells in villous stroma showed positive CD68 expression. Immunostaining Findings: CD68 showed a granular staining pattern in the cytoplasm of Hofbauer cells. The group with the highest CD68 positive cell number was HELLP group and the number of cells per cell (1.46 ± 0.25) was significantly different from all groups. CD68 positive cell count in the placental villus were the highest in HELLP group and the number of Hofbauer cells per villus was significantly different from the other groups.

Keywords

Hofbauer cells, CD68, preeclampsia, HELLP syndrome, gestational diabetes

Supporting Institution

dicle university

Project Number

TIP.16.017

References

• [1] Chang, M.D.Y., Pollard, J.W., et al. “Mouse placental macrophages have a decreased ability to present antigen”, Proceedings of the National Academy of Sciences, 90, 462-466, 1993.
• [2] Fox, H. “The incidence and significance of Hofbauer cells in the mature human placenta”, Journal of Patholology and Bacteriology, 93,710-717, 1997.
• [3] Hofbauer, J. “The function of the Hofbauer cells of the chorionic villus, particularly in relation to acute infection and syphilis”. Am J Obstet Gynecol, 10(1):1-14,1975.
• [4] Enders, AC., King, BF. “The cytology of Hofbauer cells”. Anat Rec, 167(2):231-6,1975.
• [5] Vinnars, MT., Rindsjo, E., Ghazi, S., Sundberg, A., Papadogiannakis, N. “ The number of CD68(+) (Hofbauer) cells is decreased in placentas with chorioamnionitis and with advancing gestational age”. Pediatr Dev Pathol, 13(4):300-4, 2010.
• [6] Demir, R., Erbengi, T. “Some new findings about Hofbauer cells in the chorionic villi of the human placenta”. Acta Anat (Basel). 119(1):18-26,1984.
• [7] Castellucci, M., Zaccheo, D., Pescetto, G. “A three-dimensional study of the normal human placenta villous core. I. The Hofbauer cells”. Cell Tissue Res, 210(2):235-47,1980.
• [8] Kondi-Pafiti, A., Grigoriadis, C., Samiotaki, D., Filippidou-Giannopoulou, A., Kleanthis, C., Hassiakos, D. “Immunohistochemical study of inhibin A and B expression in placentas from normal and pathological gestations”. Clin Exp Obstet Gynecol, 40(1):109-12, 2013.
• [9] King, BF. “Ultrastructural differentiation of stromal and vascular components in early macaque placental villi”. Am J Anat, 1987;178(1):30-44,1987.
• [10] Wynn, RM. “Derivation and ultrastructure of the so-called Hofbauer cell”. Am J Obstet Gynecol, 97(2):235-48,1967.
• [11] Martinoli, C., Castellucci, M., Zaccheo, D., Kaufmann, P. “Scanning electron microscopy of stromal cells of human placental villi throughout pregnancy”. Cell Tissue Res. 235(3):647-55,1984.
• [12] Hauguel-de Mouzon, S., Guerre-Millo, M. “The placenta cytokine network and inflammatory signals”. Placent, 27(8):794-82006.
• [13] Wood, GW., King, GR Jr. “Trapping antigen-antibody complexes within the human placenta”. Cell Immunol, 69(2):347-62,1982.
• [14] Wetzka, B., Clark, DE., Charnock-Jones, DS., Zahradnik, HP., Smith, SK. “Isolation of macrophages (Hofbauer cells) from human term placenta and their prostaglandin E2 and thromboxane production”. Hum Reprod, 12(4):847-52,1997.
• [15] Tang, Z., Tadesse, S., Norwitz, E, Mor, G, Abrahams, VM., Guller, S. “Isolation of Hofbauer cells from human term placentas with high yield and purity”. Am J Reprod Immunol, 66(4):336-48,2011.
• [16] Seval, Y., Korgun, E.T., et al.“Hofbauer cells in early human placenta: possible implications in vasculogenesis and angiogenesis”, Placenta, 28, 841-845, 2007.
• 17] Anteby, EY., Natanson-Yaron, S., Greenfield, C., Goldman-Wohl ,D., Haimov-Kochman, R., Holzer, H., et al. “Human placental Hofbauer cells express sprouty proteins: a possible modulating mechanism of villous branching”. Placenta, 26(6):476-83, 2005
• [18] Vinnars, MT., Rindsjo, E., Ghazi S., Sundberg, A. Papadogiannakis N. “The number of CD68(+) (Hofbauer) cells is decreased in placentas with chorioamnionitis and with advancing gestational age”.. Pediatr Dev Pathol, 13:300–4. 10, 2010. [19] Ben Amara, A., Gorvel, L., Baulan, K., Derain-Court, J., Buffat, C., Verollet,C.et al. “Placental macrophages are impaired in chorioamnionitis, an infectious pathology of the placenta”.. J Immunol. 191:5501–5514.2013.
• 20] Vinnars, M.T., Wijnaendts, L.C., Westgren, M., Bolte, A.C. Papadogiannakis, N., Nasiell, J. “ Severe preeclampsia with and without HELLP differ with regard to placental pathology”.. Hypertension 51:1295–1299, 2008.
• [21] Evsen, M.S., Kalkanli, S., Deveci, E., Sak, ME., Ozler., A, Baran, O. et al. “ Human placental macrophages (Hofbauer cells) in severe preeclampsia complicated by HELLP syndrome:v immunohistochemistry of chorionic vill”.i. Anal Quant Cytopathol Histpathol. 35:283–288,2013.
• [22] Yang, S.W., Cho, E.H., Choi, S.Y., Lee, Y.K., Park, J.H., Kim, M.K. et al. “expression in Hofbauer cells may play an important role in immune tolerance in fetal chorionic villi during the development of preeclampsia”... J Reprod Immunol. 124:30–37,2017.
• [23] Tang, Z., Buhimschi, I.A., Buhimschi, C.S., Tadesse, S., Norwit, E., Niven-Fairchild, T.et al. “Decreased levels of folate receptor-beta and reduced numbers of fetal macrophages (Hofbauer cells) in placentas from pregnancies with severe pre-eclampsia”.. Am J Reprod Immunol. 70:104–115,2013.
• [24] Demir, R., Kayisli, UA., Seval, Y., Celik-Ozenci, C., Korgun, ET., Demir-Weusten, AY., et al. Sequential expression of VEGF and its receptors in human placental villi during very early pregnancy: differences between placental vasculogenesis and angiogenesi”.s. Placenta, 25:560–572, 2004.