EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES

Erhan Mustafa Akarsu; Halit Kızılet; Pinar Aksu Kılıçle; Ali Aslan

doi:10.51477/mejs.1661476

EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES

Abstract

Environmental pollutants and genotoxic agents pose serious risks to human health and the ecosystem through DNA damage. This study aimed to evaluate the protective potential of the aqueous extract of Xanthoparmelia somloensis (XSG) lichen against ethyl methanesulfonate (EMS) and mitomycin-C (MMC) induced genotoxicity by in vitro micronucleus (MN) test. The genotoxic effects of EMS and MMC and the antigenotoxic activity of XSG extract were analyzed in peripheral blood lymphocytes taken from healthy donors. In the experimental groups determined by preliminary experiments, negative control (distilled water), positive controls (3 μg/ml EMS and MMC) and combinations containing different concentrations (30, 60, 120 μg/ml) of XSG extract were used. According to the results, MN percentages were significantly higher in EMS and MMC applied groups compared to negative control (p<0.05). XSG water extract did not show genotoxic effect when applied alone, but reduced MN frequency dose-dependently in combination with EMS and MMC. Especially 120 μg/ml XSG extract exhibited the highest antigenotoxic effect by reducing EMS-induced MN by 65.7% and MMC-induced MN by 71.7%. The protective mechanism of XSG may be related to the scavenging of free radicals and modulation of DNA repair pathways through antioxidant components (e.g. usnic acid). These findings reveal the potential of XSG as a broad-spectrum antigenotoxic agent and contribute to the development of natural protective strategies.

Keywords

Supporting Institution

none

Project Number

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Ethical Statement

The study was approved by the Kafkas University Faculty of Medicine Ethics Committee with the approval numbered 27.04.2017/97.

Thanks

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Details

Primary Language

English

Subjects

Cancer Biology, Genotoxicity and Cytotoxicity, Cell and Nuclear Division, Cytogenetic, Medicinal and Aromatic Plants

Journal Section

Research Article

Authors

Erhan Mustafa Akarsu
0000-0002-6956-0909
Türkiye

Halit Kızılet ^*
0000-0002-7289-4635
Türkiye

Pinar Aksu Kılıçle
0000-0002-3567-5775
Türkiye

Ali Aslan
0000-0002-5122-6646
Türkiye

Publication Date

December 30, 2025

Submission Date

March 19, 2025

Acceptance Date

November 18, 2025

Published in Issue

Year 2025 Volume: 11 Number: 2

DOI

https://doi.org/10.51477/mejs.1661476

IZ

https://izlik.org/JA23DD62FD

Cite

RIS / Bibtex

APA

Akarsu, E. M., Kızılet, H., Aksu Kılıçle, P., & Aslan, A. (2025). EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. Middle East Journal of Science, 11(2), 142-154. https://doi.org/10.51477/mejs.1661476

AMA

1.Akarsu EM, Kızılet H, Aksu Kılıçle P, Aslan A. EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. MEJS. 2025;11(2):142-154. doi:10.51477/mejs.1661476

Chicago

Akarsu, Erhan Mustafa, Halit Kızılet, Pinar Aksu Kılıçle, and Ali Aslan. 2025. “EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia Somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES”. Middle East Journal of Science 11 (2): 142-54. https://doi.org/10.51477/mejs.1661476.

EndNote

Akarsu EM, Kızılet H, Aksu Kılıçle P, Aslan A (December 1, 2025) EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. Middle East Journal of Science 11 2 142–154.

IEEE

[1]E. M. Akarsu, H. Kızılet, P. Aksu Kılıçle, and A. Aslan, “EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES”, MEJS, vol. 11, no. 2, pp. 142–154, Dec. 2025, doi: 10.51477/mejs.1661476.

ISNAD

Akarsu, Erhan Mustafa - Kızılet, Halit - Aksu Kılıçle, Pinar - Aslan, Ali. “EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia Somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES”. Middle East Journal of Science 11/2 (December 1, 2025): 142-154. https://doi.org/10.51477/mejs.1661476.

JAMA

1.Akarsu EM, Kızılet H, Aksu Kılıçle P, Aslan A. EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. MEJS. 2025;11:142–154.

MLA

Akarsu, Erhan Mustafa, et al. “EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia Somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES”. Middle East Journal of Science, vol. 11, no. 2, Dec. 2025, pp. 142-54, doi:10.51477/mejs.1661476.

Vancouver

1.Erhan Mustafa Akarsu, Halit Kızılet, Pinar Aksu Kılıçle, Ali Aslan. EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. MEJS. 2025 Dec. 1;11(2):142-54. doi:10.51477/mejs.1661476