INVESTIGATION OF TEMPERATURE DEPENDENCE OF D2O SOLUTIONS BY 400 MHZ NMR

Year 2018, Volume: 4 Issue: 1, 1 - 6, 04.06.2018

Gülten Kavak Balcı Canan Aytuğ Ava Ali Yılmaz

https://doi.org/10.23884/mejs.2018.4.1.01

Cited By: 1

Abstract

The
rate of water proton relaxation of protein solutions were studied
in the presence and absence of the paramagnetic ions[gadolinium (III), manganese (II),
chromium (III), iron (III), nickel (II), copper (II), and cobalt (II)]   in the previous studies. However,
these studies were carried out rather at low frequencies. Therefore, studying
of temperature dependence of relaxation rates for absence and presence of 2 %
albumin in pure D₂O by 400 MHz will be a novelty.

In this study, T₁ and T₂ relaxation
ratios of D₂O and 0.1 H₂O/0.9D₂O solutions were investigated with respect to
temperature for pure and  for
constant  albumin  concentration(2%). The experiments were
carried out by using Bruker Avance 400 MHz NMR. Inversion Recovery (180-τ-90)
pulse step were used for T₁, whereas Carr-Purcell-Meiboom-Gill pulse
step were used for T₂.  The
experiments were performed for temperature range of 20°C-40°C by using
automatic temperature control unit.

1/T₁ and 1/T₂
decrease linearly with increasing temperature for pure D₂O
solutions. However, for 0.1H₂O/0.9D₂O solutions, the
relaxation rates of T₁ increase with increasing temperature while T₂
decreases with increasing temperature. The
decrease in both relaxation rates of the D₂O solution with respect
to the increased temperature suggests that relaxation is due to spin relaxation
interaction. Increasing of relaxation rates with the increasing temperature, in
the presence of albumin demonstrates the validity of the
dipolar mechanism

Keywords

NMR, T1, T2, Relaxation, Albumin

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