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Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae)

Year 2021, Volume: 21 Issue: 1, 49 - 58, 15.07.2021

Abstract

Honey bee colonies, in particular Apis mellifera intermissa, are affected by a Varroa destructor mite which threatens them with extinction. Several chemical treatments are used but the parasite has become resistant to them. To this end, in recent years, substances of plant origin have been used as a natural alternative means of combating this parasite. In the present work, the acaricidal effect of propolis (which is a product of the hive) is evaluated in the laboratory. The results obtained show that the extract of propolis with 70% ethanol (EEP) is highly toxic against varroa. In fact, 40% to 100% mortality is obtained respectively after 30 minutes to 120 minutes of exposure of the varroa mite to this extract. On the other hand, spraying mites with 100 mg of powdered propolis shows a 20% to 100% mortality after 12 to 24 hours of exposure. In addition, exposure of bees to EEP or propolis powder does not cause any mortality.

References

  • Amaros, M., Sauvager, F., Girre, L.Et Cormier, M., 1992. In vitro antiviral activity of propolis. Apidologie 23, 231-40.
  • Antunez K, Harriet J, Gende L, Magi M, Eguaras M, Zunino P., 2008. Efficacy of natural propolis extract in the control of American Foulbrood. Vet Microbiol 131:324–331.
  • Bastos, E.M., Simone, M., Jorge, D.M., Soares, A.E., Spivak, A.M., 2008. In vitro studyof the antimicrobial activity of Brazilian propolis against Paenibacilluslarvae. Journal of Invertebrate Pathology 97, 273–281.
  • Bogdanov, S. ,2011: Propolis: Composition, Health, Medicine: A Review. Bee Product Science.
  • Boyanova L., Kolarov R., Gergova G. Et Mitov I., 2006. In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe, 12:173-177.
  • Bruneau E., 2002. Les produits de la ruche. Le traité de rustica de l’apiculture. Ed. Rustica, Paris, 354-416p.
  • Bufalo, M.C., Candeias, J.M.G., Sforcin, J.M., 2009. In vitro cytotoxic effect of Braziliangreen propolis on human laryngeal epidermoide carcinoma (HEp-2) cells. Evidence-based Complementary and Alternative Medicine 6, 483–487.
  • Burdock G.A., 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem.Toxicol.3 (6): 347-363.
  • Damiani N., Fernandez N.J., Maldonado L.M., Alvarez A.R., Eguaras M.J. Etmarcangeli J.A., 2010. Bioactivity of propolis from different geographical origins on Varroa destructor (Acari: Varroidae). Parasitol Res. 107:31-37.
  • Dimov V., Ivanovska N., Manolova N., Bankova V., Nikolov N., Popov S., 1991. Immunomodulatory action of propolis Influence on antiinfectious protection and macrophage function. Apidologie, 22:155–162.
  • Drago, L., Mombelli, B., Vecchi, E., Fascina, M., Tocalli, Et Gismondo, M., 2000.In vitro antimicrobial activity of propolis dry extract. J. Chemother. 12, 390–395.
  • Drescher N., Klein A.M., Neumann P., Yañez O., Et Leonhardt S.D. 2017. Inside Honeybee Hives: Impact of Natural Propolis on the Ectoparasitic Mite Varroa destructor and Viruses. Insects, 8, 15.
  • Freitas, S.F., Shinohara, L., Sforcin, J.M. Et Guimaraes, S., 2006. In vitro effects of propolison Giardia duodenalistrophozoites. Phytomedicine 13, 170–175.
  • Garedew A., Lamprecht I., Schmolz E. Et Schricker B. 2002. The varroacidal action of propolis: a laboratory assay. Apidologie 33: 41–50.
  • Garedew, A., Schmolza, E., Lamprechtb, I., 2004. Microbiological and calorimetric investigations on the antimicrobial actions of different propolis extracts: an in vitro approach. Thermochim.Acta 422, 115–124
  • Gekker G., Hu S.X., Spivak M., Lokensgard J.R. Et Peterson P.K., 2005. Anti-HIV-1 activity of propolis in CD4 (+) lymphocyte and microglial cell cultures. J. thnopharmacol. 102:158–163.
  • Ghisalberti E.L. 1979. Propolis: a review, BeeWorld 60, 58–84.
  • Grange J.M. Davey R.W., 1990. Antibacterial properties of propolis. Journal of the Royal Society of Medicine, 83:159-60.
  • Hegazi, A.G. Abd El Hady, F.K., 2002. Egyptian propolis: 3.Antioxidant, antimicrobial activities and chemical composition of propolis from reclaimed lands. Zeitschrift fur Naturforschung - Section C J. Biosci., 57 (3-4), pp. 395-402.
  • Higashi K. O. De Castro S. L., 1995. Effect of different formulations of propolis on mice infected with Trypanosoma cruzi. J. Ethnopharmamcology 46, 55-8.
  • Kedzia A., 1986. Effect of Ethanol extract of propolis (EEP) on anaerobic bacteria. Herbapolonica, 32 (1): 53-58.
  • Kujumgiev A., Tsvetkova I., Serkedjieva Y., Bankova V., Et Christov R.,1999. Antibacterial, Antifungal and Antiviral Activity of Propolis of Different Geographic Origin.J. Ethnopharmacol : 64(3):235-40.
  • Marcucci, M.C., 1995. Propolis: chemical composition, biological properties and therapeutical activity, Apidologie, 26: 83–99.
  • Milani N., 1995. The resistance of Varroa jacobsoni Oud, to pyrethroids: a laboratory assay, Apidologie 26, 415-429.
  • Miyataka H., Nishiki M., Matsumoto H., Fujimoto T., Matsuka M., Et Sotah, T., 1997. Evaluation of propolis I. Evaluation of Brazilian and Chinese propolis by enzymatic and physico-chemical methods. Biol Pharm Bull 20, 497-501.
  • Murad J.M., Calvi S.A., Soares A.M., Bankova V. Et Sforcin J.M., 2002. Effects of propolis from Brazil and Bulgaria on fungicidal activity of macrophages against Paracoccidioides brasiliensis. J Ethnopharmacol. 79(3) :331-441.
  • Ota C., Unterkircher C., Fantinato V. Et Shimizu M.T., 2001. Antifungal activity of propolis on different species of Candida. Mycoses, 44: 375-8.
  • Ozcan M., Unver A., Ceylan D.A. Et Yetisir R., 2004. Inhibitory effect of pollen and propolis extracts Nahrung. 48(3):188-194.
  • Popov S., 1999. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology 64 : 235–240.
  • Popova, M., Reyes, M., Le Conte, Y., Bankova, V., 2014. Propolis chemical composition and honeybee resistance against Varroa destructor. Nat. Prod. Res., 28, 788-794.
  • Prost J.P., Le Conte Y., 2005. Apiculture : connaître l’abeille, conduire le rucher. Ed Lavoisier, Paris, 698p.
  • Rojas Hernandez N. M., Candelario M. Et Olivares E., 1993. Antimicrobial activity of propolis against representatives of the genus Mycobacterium. Revista Biologia (Habana), 7 (1): 69-75.
  • Sforcin, J.M., Fernandes Jr., A., Lopes, C.A.M., Bankova, V. Et Funari, S.R.C., 2000.Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology73, 243–249.
  • Sforcinj.M., Fernandes Jr., A., Lopes C.A.M., Funaris.R.C., Et Bankova V., 2001. Seasonal effect of Brazilian propolis on Candida albicans and Candida tropicalis. Journal of Venomous Animals and Toxins 7, 139–144.
  • Sforcin J.M., 2007. Propolis and the immune system: a review.J. Ethnopharmacol. 113, 1–14. Strehl E., Volpert R. Et Elstner E.F., 1994. Biochemical activities of propolis extracts: III Inhibition of dihydrofolate reductase, Z. Naturforsch. 49c, 39–43.
  • Ugur A. Et Arslan T., 2004. An in vitro study on antimicrobial activity of propolis from Mugla province of Turkey. Med Food, 7: 90-4.
  • Velikova M., V. Bankova V., Sorkunb K., Tsetkova I. Saadi H. Et Kujumgiev A., 2000. Propolis from the Mediterranean Region: Chemical Composition and Antimicrobial activity. Fitoterapia. 71, 693-696.
  • Wilson M.B., Brinkman D., Spivak M., Gardner G. Et Cohen J.D., 2015. Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvae and Ascosphaera apis. J. Invertebr. Pathol. 124:44–50.
Year 2021, Volume: 21 Issue: 1, 49 - 58, 15.07.2021

Abstract

References

  • Amaros, M., Sauvager, F., Girre, L.Et Cormier, M., 1992. In vitro antiviral activity of propolis. Apidologie 23, 231-40.
  • Antunez K, Harriet J, Gende L, Magi M, Eguaras M, Zunino P., 2008. Efficacy of natural propolis extract in the control of American Foulbrood. Vet Microbiol 131:324–331.
  • Bastos, E.M., Simone, M., Jorge, D.M., Soares, A.E., Spivak, A.M., 2008. In vitro studyof the antimicrobial activity of Brazilian propolis against Paenibacilluslarvae. Journal of Invertebrate Pathology 97, 273–281.
  • Bogdanov, S. ,2011: Propolis: Composition, Health, Medicine: A Review. Bee Product Science.
  • Boyanova L., Kolarov R., Gergova G. Et Mitov I., 2006. In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe, 12:173-177.
  • Bruneau E., 2002. Les produits de la ruche. Le traité de rustica de l’apiculture. Ed. Rustica, Paris, 354-416p.
  • Bufalo, M.C., Candeias, J.M.G., Sforcin, J.M., 2009. In vitro cytotoxic effect of Braziliangreen propolis on human laryngeal epidermoide carcinoma (HEp-2) cells. Evidence-based Complementary and Alternative Medicine 6, 483–487.
  • Burdock G.A., 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem.Toxicol.3 (6): 347-363.
  • Damiani N., Fernandez N.J., Maldonado L.M., Alvarez A.R., Eguaras M.J. Etmarcangeli J.A., 2010. Bioactivity of propolis from different geographical origins on Varroa destructor (Acari: Varroidae). Parasitol Res. 107:31-37.
  • Dimov V., Ivanovska N., Manolova N., Bankova V., Nikolov N., Popov S., 1991. Immunomodulatory action of propolis Influence on antiinfectious protection and macrophage function. Apidologie, 22:155–162.
  • Drago, L., Mombelli, B., Vecchi, E., Fascina, M., Tocalli, Et Gismondo, M., 2000.In vitro antimicrobial activity of propolis dry extract. J. Chemother. 12, 390–395.
  • Drescher N., Klein A.M., Neumann P., Yañez O., Et Leonhardt S.D. 2017. Inside Honeybee Hives: Impact of Natural Propolis on the Ectoparasitic Mite Varroa destructor and Viruses. Insects, 8, 15.
  • Freitas, S.F., Shinohara, L., Sforcin, J.M. Et Guimaraes, S., 2006. In vitro effects of propolison Giardia duodenalistrophozoites. Phytomedicine 13, 170–175.
  • Garedew A., Lamprecht I., Schmolz E. Et Schricker B. 2002. The varroacidal action of propolis: a laboratory assay. Apidologie 33: 41–50.
  • Garedew, A., Schmolza, E., Lamprechtb, I., 2004. Microbiological and calorimetric investigations on the antimicrobial actions of different propolis extracts: an in vitro approach. Thermochim.Acta 422, 115–124
  • Gekker G., Hu S.X., Spivak M., Lokensgard J.R. Et Peterson P.K., 2005. Anti-HIV-1 activity of propolis in CD4 (+) lymphocyte and microglial cell cultures. J. thnopharmacol. 102:158–163.
  • Ghisalberti E.L. 1979. Propolis: a review, BeeWorld 60, 58–84.
  • Grange J.M. Davey R.W., 1990. Antibacterial properties of propolis. Journal of the Royal Society of Medicine, 83:159-60.
  • Hegazi, A.G. Abd El Hady, F.K., 2002. Egyptian propolis: 3.Antioxidant, antimicrobial activities and chemical composition of propolis from reclaimed lands. Zeitschrift fur Naturforschung - Section C J. Biosci., 57 (3-4), pp. 395-402.
  • Higashi K. O. De Castro S. L., 1995. Effect of different formulations of propolis on mice infected with Trypanosoma cruzi. J. Ethnopharmamcology 46, 55-8.
  • Kedzia A., 1986. Effect of Ethanol extract of propolis (EEP) on anaerobic bacteria. Herbapolonica, 32 (1): 53-58.
  • Kujumgiev A., Tsvetkova I., Serkedjieva Y., Bankova V., Et Christov R.,1999. Antibacterial, Antifungal and Antiviral Activity of Propolis of Different Geographic Origin.J. Ethnopharmacol : 64(3):235-40.
  • Marcucci, M.C., 1995. Propolis: chemical composition, biological properties and therapeutical activity, Apidologie, 26: 83–99.
  • Milani N., 1995. The resistance of Varroa jacobsoni Oud, to pyrethroids: a laboratory assay, Apidologie 26, 415-429.
  • Miyataka H., Nishiki M., Matsumoto H., Fujimoto T., Matsuka M., Et Sotah, T., 1997. Evaluation of propolis I. Evaluation of Brazilian and Chinese propolis by enzymatic and physico-chemical methods. Biol Pharm Bull 20, 497-501.
  • Murad J.M., Calvi S.A., Soares A.M., Bankova V. Et Sforcin J.M., 2002. Effects of propolis from Brazil and Bulgaria on fungicidal activity of macrophages against Paracoccidioides brasiliensis. J Ethnopharmacol. 79(3) :331-441.
  • Ota C., Unterkircher C., Fantinato V. Et Shimizu M.T., 2001. Antifungal activity of propolis on different species of Candida. Mycoses, 44: 375-8.
  • Ozcan M., Unver A., Ceylan D.A. Et Yetisir R., 2004. Inhibitory effect of pollen and propolis extracts Nahrung. 48(3):188-194.
  • Popov S., 1999. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology 64 : 235–240.
  • Popova, M., Reyes, M., Le Conte, Y., Bankova, V., 2014. Propolis chemical composition and honeybee resistance against Varroa destructor. Nat. Prod. Res., 28, 788-794.
  • Prost J.P., Le Conte Y., 2005. Apiculture : connaître l’abeille, conduire le rucher. Ed Lavoisier, Paris, 698p.
  • Rojas Hernandez N. M., Candelario M. Et Olivares E., 1993. Antimicrobial activity of propolis against representatives of the genus Mycobacterium. Revista Biologia (Habana), 7 (1): 69-75.
  • Sforcin, J.M., Fernandes Jr., A., Lopes, C.A.M., Bankova, V. Et Funari, S.R.C., 2000.Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology73, 243–249.
  • Sforcinj.M., Fernandes Jr., A., Lopes C.A.M., Funaris.R.C., Et Bankova V., 2001. Seasonal effect of Brazilian propolis on Candida albicans and Candida tropicalis. Journal of Venomous Animals and Toxins 7, 139–144.
  • Sforcin J.M., 2007. Propolis and the immune system: a review.J. Ethnopharmacol. 113, 1–14. Strehl E., Volpert R. Et Elstner E.F., 1994. Biochemical activities of propolis extracts: III Inhibition of dihydrofolate reductase, Z. Naturforsch. 49c, 39–43.
  • Ugur A. Et Arslan T., 2004. An in vitro study on antimicrobial activity of propolis from Mugla province of Turkey. Med Food, 7: 90-4.
  • Velikova M., V. Bankova V., Sorkunb K., Tsetkova I. Saadi H. Et Kujumgiev A., 2000. Propolis from the Mediterranean Region: Chemical Composition and Antimicrobial activity. Fitoterapia. 71, 693-696.
  • Wilson M.B., Brinkman D., Spivak M., Gardner G. Et Cohen J.D., 2015. Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvae and Ascosphaera apis. J. Invertebr. Pathol. 124:44–50.
There are 38 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Articles
Authors

Cherifi Assia

Noureddine Adjlane 0000-0002-0369-2968

Publication Date July 15, 2021
Submission Date March 4, 2021
Acceptance Date July 15, 2021
Published in Issue Year 2021 Volume: 21 Issue: 1

Cite

APA Assia, C., & Adjlane, N. (2021). Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae). Mellifera, 21(1), 49-58.
AMA Assia C, Adjlane N. Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae). mellifera. July 2021;21(1):49-58.
Chicago Assia, Cherifi, and Noureddine Adjlane. “Preliminary Trials on the Efficacy of Propolis in the Control of the Varroa Destructor (Mesostigmata Vrroidae) Ectoparasite of the Honey Bee Apis Mellifera Intermissa (Hymenoptera: Apidae)”. Mellifera 21, no. 1 (July 2021): 49-58.
EndNote Assia C, Adjlane N (July 1, 2021) Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae). Mellifera 21 1 49–58.
IEEE C. Assia and N. Adjlane, “Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae)”, mellifera, vol. 21, no. 1, pp. 49–58, 2021.
ISNAD Assia, Cherifi - Adjlane, Noureddine. “Preliminary Trials on the Efficacy of Propolis in the Control of the Varroa Destructor (Mesostigmata Vrroidae) Ectoparasite of the Honey Bee Apis Mellifera Intermissa (Hymenoptera: Apidae)”. Mellifera 21/1 (July 2021), 49-58.
JAMA Assia C, Adjlane N. Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae). mellifera. 2021;21:49–58.
MLA Assia, Cherifi and Noureddine Adjlane. “Preliminary Trials on the Efficacy of Propolis in the Control of the Varroa Destructor (Mesostigmata Vrroidae) Ectoparasite of the Honey Bee Apis Mellifera Intermissa (Hymenoptera: Apidae)”. Mellifera, vol. 21, no. 1, 2021, pp. 49-58.
Vancouver Assia C, Adjlane N. Preliminary trials on the efficacy of propolis in the control of the Varroa destructor (Mesostigmata Vrroidae) ectoparasite of the honey bee Apis mellifera intermissa (Hymenoptera: Apidae). mellifera. 2021;21(1):49-58.