Düzeltme: Kolşisin ve Tripsin Uygulamalarının Yetişkin Dişi ve Erkek Nil Tilapya Dokularından ve Larvalarından Elde Edilen Metafaz Kromozom Yayılımı Üzerine Etkileri

Abstract

Bu makalenin ilk hali 28.12.2024 tarihinde yayımlanmıştır. https://dergipark.org.tr/tr/pub/menba/issue/88727/1538957 Erratum to: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia. Menba, 2024; 10(3): 113–118. DOI: 10.58626/menba.1538957 Bu makalenin yayımlanan versiyonunda özet (abstract) bölümünde ve dizgide (layout/typesetting) sehven birtakım hatalar yer almıştır. Söz konusu hatalar, derginin önceki adı olan Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi ile ilgili değildir; editoryal süreçte meydana gelen teknik hatalardan kaynaklanmıştır. Ploidi, jinojenez ve androjenez gibi su ürünleri yetiştiriciliğinde kullanılan farklı biyoteknolojik yöntemlerin sonuçlarının değerlendirilmesi için karyotip ve kromozom sayısı gibi klasik sitogenetik tekniklere ihtiyaç vardır. İyi dağılmış metafaz kromozom yayılımları elde etmek, sitogenetik teknikler için önemli bir adımdır. Bu çalışmanın amacı, kolşisin ve tripsin tedavilerinin sayılabilir metafaz kromozom yayılımlarının verimi üzerindeki etkilerini değerlendirmekti. Bu amaçla, yetişkin dişi ve erkek Nil tilapyasının (Oreochromis niloticus) solungaç, dalak, böbrek ve kuyruk yüzgeci dokularından elde edilen kromozom preparatları ile larvalarının tüm vücudundan elde edilen kromozom preparatları değerlendirildi. Şu ana kadar incelenen önceki çalışmalara dayanarak, bu, cinsiyet farklılıkları ve larvalar açısından kolşisin ve tripsinin metafaz kromozom yayılımları üzerindeki etkilerini gösteren ilk sistematik ve kapsamlı çalışmadır. Kromozom hazırlıklarında dört işlem "Tripsin (-) Kolşisin (-)", "Tripsin (-) Kolşisin (+)", "Tripsin (+) Kolşisin (-)" ve "Tripsin (+) Kolşisin (+)" olarak belirlenmiştir. Kolşisin ve tripsinin kromozom hazırlıklarında kullanımı açısından etkileri, metafaz hücrelerinin sıklıklarının belirlenmesiyle değerlendirildi. Bu amaçla, tedaviler arasında metafaz hücrelerinin tespitindeki farklılıkları ortaya çıkarmak için bir metafaz indeksi kullanıldı. En yüksek metafaz indeksi değerleri, tüm dokularda Tripsin (+) Kolşisin (+) tedavi grubunda hesaplandı. Ayrıca, dalak ve böbrek dokularında bulunan metafaz indeksi skorları, solungaç ve yüzgeç dokularında bulunanlardan daha yüksekti. Bu çalışma aynı zamanda, metafaz kromozom yayılımlarının kolşisin veya tripsin uygulamaları olmadan elde edilebileceğini göstermiştir.

Erratum to: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia

Abstract

The first version of this article was published on 28.12.2024. https://dergipark.org.tr/tr/pub/menba/issue/88727/1538957 Erratum to: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia. Menba, 2024; 10(3): 113–118. DOI: 10.58626/menba.1538957 In the published version of this article, misswriten were inadvertently introduced in the abstract section and in the typesetting (layout). These mistakes were not related to the journal’s former title, Menba Kastamonu University Faculty of Fisheries Journal, but resulted from technical issues during the editorial and production process. Assessment of the results of different biotechnological methods in aquaculture such as ploidy, gynogenesis, and androgenesis are needed classical cytogenetical techniques like karyotype and chromosome number. Achieving well-distributed metaphase chromosome spreads is a preeminent step for the cytogenetical techniques. The aim of this study was to evaluate the effects of colchicine and trypsin treatments on the yield of countable metaphase chromosome spreads. With this aim, chromosome preparations from gill, spleen, kidney, and caudal fin tissues of adult females and males Nile tilapia (Oreochromis niloticus) as well as from whole body of their larvae were evaluated. Based on the previous studies reviewed so far, this is the first systematic and extensive study indicated that the effects of colchicine and trypsin on metaphase chromosome spreads regarding gender differences and larvae. In the chromosome preparations, it was designated four treatments as “Trypsin (-) Colchicine (-)”, “Trypsin (-) Colchicine (+)”, “Trypsin (+) Colchicine (-)”, and “Trypsin (+) Colchicine (+)”. Effects of colchicine and trypsin in terms of their usage in chromosome preparations were assessed by determining the frequencies of metaphase cells. For this purpose, a metaphase index was used to reveal differences in detection of metaphase cells among the treatments. The highest metaphase index values were calculated in the Trypsin (+) Colchicine (+) treatment group of all tissues. Also, the scores of metaphase index found in spleen and kidney tissues were greater than those found in gill and fin tissues. The present study has also shown that metaphase chromosome spreads could be obtained without colchicine or trypsin treatments.

Keywords

• Metaphase spread
• Chromosome
• Colchicine
• Trypsin
• Nile Tilapia (Oreochromis niloticus)

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