Erratum: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia

Year 2025, Volume: 11 Issue: 4, 416 - 422, 31.12.2025

Burak Evren İnanan

https://doi.org/10.58626/memba.1750255

Abstract

The first version of this article was published on 28.12.2024. https://dergipark.org.tr/tr/pub/menba/issue/88727/1538957
Erratum to: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia. Menba, 2024; 10(3): 113–118. DOI: 10.58626/menba.1538957
In the published version of this article, misswriten were inadvertently introduced in the abstract section and in the typesetting (layout). These mistakes were not related to the journal’s former title, Menba Kastamonu University Faculty of Fisheries Journal, but resulted from technical issues during the editorial and production process.

Assessment of the results of different biotechnological methods in aquaculture such as ploidy, gynogenesis, and androgenesis are needed classical cytogenetical techniques like karyotype and chromosome number. Achieving well-distributed metaphase chromosome spreads is a preeminent step for the cytogenetical techniques. The aim of this study was to evaluate the effects of colchicine and trypsin treatments on the yield of countable metaphase chromosome spreads. With this aim, chromosome preparations from gill, spleen, kidney, and caudal fin tissues of adult females and males Nile tilapia (Oreochromis niloticus) as well as from whole body of their larvae were evaluated. Based on the previous studies reviewed so far, this is the first systematic and extensive study indicated that the effects of colchicine and trypsin on metaphase chromosome spreads regarding gender differences and larvae. In the chromosome preparations, it was designated four treatments as “Trypsin (-) Colchicine (-)”, “Trypsin (-) Colchicine (+)”, “Trypsin (+) Colchicine (-)”, and “Trypsin (+) Colchicine (+)”. Effects of colchicine and trypsin in terms of their usage in chromosome preparations were assessed by determining the frequencies of metaphase cells. For this purpose, a metaphase index was used to reveal differences in detection of metaphase cells among the treatments. The highest metaphase index values were calculated in the Trypsin (+) Colchicine (+) treatment group of all tissues. Also, the scores of metaphase index found in spleen and kidney tissues were greater than those found in gill and fin tissues. The present study has also shown that metaphase chromosome spreads could be obtained without colchicine or trypsin treatments.

Keywords

Metaphase spread , Chromosome , Colchicine , Trypsin , Nile Tilapia (Oreochromis niloticus)

Ethical Statement

This study was conducted in Aksaray University Experimental Animal Application and Research Center (ASÜ-DEHAM) with the approval number 2021/1-3.

Supporting Institution

This study was financially supported by Aksaray University Coordinatorship of Scientific Research Projects with the project number of 2021/021.

Project Number

Aksaray University Coordinatorship of Scientific Research Projects (2021/021)

Thanks

The author would like to thank Prof. Dr. Mustafa Öz from Aksaray University for his assistance with the samplings. Also, the author is especially grateful to Prof. Dr. Tahir Karaşahin for the opportunity to examine the sections under the fluorescence microscope at Aksaray University Embryo Transfer Training Application Research Center.

Düzeltme: Kolşisin ve Tripsin Uygulamalarının Yetişkin Dişi ve Erkek Nil Tilapya Dokularından ve Larvalarından Elde Edilen Metafaz Kromozom Yayılımı Üzerine Etkileri

Abstract

Bu makalenin ilk hali 28.12.2024 tarihinde yayımlanmıştır. https://dergipark.org.tr/tr/pub/menba/issue/88727/1538957
Erratum to: Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia. Menba, 2024; 10(3): 113–118. DOI: 10.58626/menba.1538957
Bu makalenin yayımlanan versiyonunda özet (abstract) bölümünde ve dizgide (layout/typesetting) sehven birtakım hatalar yer almıştır. Söz konusu hatalar, derginin önceki adı olan Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi ile ilgili değildir; editoryal süreçte meydana gelen teknik hatalardan kaynaklanmıştır.

Ploidi, jinojenez ve androjenez gibi su ürünleri yetiştiriciliğinde kullanılan farklı biyoteknolojik yöntemlerin sonuçlarının değerlendirilmesi için karyotip ve kromozom sayısı gibi klasik sitogenetik tekniklere ihtiyaç vardır. İyi dağılmış metafaz kromozom yayılımları elde etmek, sitogenetik teknikler için önemli bir adımdır. Bu çalışmanın amacı, kolşisin ve tripsin tedavilerinin sayılabilir metafaz kromozom yayılımlarının verimi üzerindeki etkilerini değerlendirmekti. Bu amaçla, yetişkin dişi ve erkek Nil tilapyasının (Oreochromis niloticus) solungaç, dalak, böbrek ve kuyruk yüzgeci dokularından elde edilen kromozom preparatları ile larvalarının tüm vücudundan elde edilen kromozom preparatları değerlendirildi. Şu ana kadar incelenen önceki çalışmalara dayanarak, bu, cinsiyet farklılıkları ve larvalar açısından kolşisin ve tripsinin metafaz kromozom yayılımları üzerindeki etkilerini gösteren ilk sistematik ve kapsamlı çalışmadır. Kromozom hazırlıklarında dört işlem "Tripsin (-) Kolşisin (-)", "Tripsin (-) Kolşisin (+)", "Tripsin (+) Kolşisin (-)" ve "Tripsin (+) Kolşisin (+)" olarak belirlenmiştir. Kolşisin ve tripsinin kromozom hazırlıklarında kullanımı açısından etkileri, metafaz hücrelerinin sıklıklarının belirlenmesiyle değerlendirildi. Bu amaçla, tedaviler arasında metafaz hücrelerinin tespitindeki farklılıkları ortaya çıkarmak için bir metafaz indeksi kullanıldı. En yüksek metafaz indeksi değerleri, tüm dokularda Tripsin (+) Kolşisin (+) tedavi grubunda hesaplandı. Ayrıca, dalak ve böbrek dokularında bulunan metafaz indeksi skorları, solungaç ve yüzgeç dokularında bulunanlardan daha yüksekti. Bu çalışma aynı zamanda, metafaz kromozom yayılımlarının kolşisin veya tripsin uygulamaları olmadan elde edilebileceğini göstermiştir.

Project Number

Aksaray University Coordinatorship of Scientific Research Projects (2021/021)

References

• Bertão, M.R., & Aguiar-Perecin, M.L.R. (2002). Maize somatic chromosome preparation: pretreatments and genotypes for obtention of high index of metaphase accumulation. Caryologia, 55(2): 115-119. https://doi.org/10.1080/00087114.2002.10589266 Add to Citavi project by DOI
• Blanco, D.R., L.A.C. Bertollo, R.L. Lui, M.R. Vicari, V.P. Margarido, R.F. Artoni & O. Moreira Filho. (2012). A new technique for obtaining mitotic chromosome spreads from fishes in the field. J. Fish Biol., 81:351-357. https://doi.org/10.1111/j.1095-8649.2012.03325.x Add to Citavi project by DOI
• Cattin, P.M. & Ferreira, J.T. (1989). A rapid, nonsacrificial chromosome preparation technique for freshwater teleosts. South African Journal of Zoology, 24:76-78. https://doi.org/10.1080/02541858.1989.11448136 Add to Citavi project by DOI
• Chourrout, D., & Happe, A., (1986). Improved methods of direct chromosome preparation in rainbow trout, Salmo gairdneri. Aquaculture, 52: 255-261. https://doi.org/10.1016/0044-8486(86)90368-6 Add to Citavi project by DOI
• Demirok, N.K. & Ünlü, E., (2001). Karyotypes of Cyprinid fish Capoeta trutta and Capoeta capoeta umbla (Cyprinidae) from the Tigris River. Turkish Journal of Zoology, 25: 389-393.
• Deng, W., Tsao, S.W., Lucas, J.N., Leung, C.S., & Cheung, A.L. (2003). A new method for improving metaphase chromosome spreading. Cytometry A, 51(1):46-51. https://doi.org/10.1002/cyto.a.10004 Add to Citavi project by DOI
• Foresti, F., Oliveira, C., & Foresti de Almeida-Toledo, L. (1993). A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia, 49: 810-813. https://doi.org/10.1007/BF01923555 Add to Citavi project by DOI
• Harvey, S., Campos-Ramos, R., Kennedy, D.D., Ezaz, M.T., Bromage, N.R., Griffin, D.K., & Penman, D.J. (2002). Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis Karongae and O. Niloticus × O. Karongae Hybrids. Genetica 115:169-177. https://doi.org/10.1023/A:1020190918431 Add to Citavi project by DOI
• Henegariu, O., Heerema, N.A., Wright, L.L., Brayward, P., Ward, D.C., & Vance G.H. (2001). Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry, 43: 101-109. https://doi.org/10.1002/1097-0320(20010201)43:2<101::AID-CYTO1024>3.0.CO;2-8 Add to Citavi project by DOI
• Howe, B., Umrigar, A., & Tsien, F. (2014). Chromosome preparation from cultured cells. J. Vis. Exp., 28(83):e50203. https://doi.org/10.3791/50203 Add to Citavi project by DOI
• Hussain, M.G., & McAndrew, B.J. (1994). An improved technique for chromosome karyotyping from embryonic and soft tissues of Tilapia and Salmonids. Asian Fisheries Science, 7: 187-190.
• Moreira-Filho, O., & Bertollo, L.A.C. (1991). Extraction and use of the cephalic kidney for chromosome studies in small fi sh. Braz. J. Genet., 14:1085-1090.
• Mukti, A.T., Carman, O., Alimuddin, & Zairin Jr, M. (2016). A rapid chromosome preparation technique without metaphase arrest for ploidy determination in Nile tilapia, Oreochromis niloticus, Caryologia, 69(2):175-180. https://doi.org/10.1080/00087114.2016.1152112 Add to Citavi project by DOI
• O'Connor, C. (2008). Karyotyping for chromosomal abnormalities. Nature Education 1(1):27.
• Pope, L.H., Xiong, C., & Marko, J.F. (2006). Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymes. Mol. Biol. Cell., 17(1):104-113. https://doi.org/10.1091/mbc.e05-04-0321 Add to Citavi project by DOI
• Pradeep, P.J., Srijaya, T.C., Zain, R.B.M., Papini, A., & Chatterji, A.K. (2011). A simple technique for chromosome preparation from embryonic tissues of teleosts for ploidy verification. Caryologia, 64(2): 235-241. https://doi.org/10.1080/00087114.2002.10589788 Add to Citavi project by DOI
• Rieder, C.L., & Palazzo, R.E. (1992). Colcemid and the mitotic cycle. J. Cell Sci., 102(3):387-392. https://doi.org/10.1242/jcs.102.3.387 Add to Citavi project by DOI
• Schneider, C.A., Rasband, W.S., & Eliceiri, K.W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7):671-675. https://doi.org/10.1038/nmeth.2089 Add to Citavi project by DOI
• Supiwong, W., Tanomtong, A., Supanuam, P., Seetapan, K., Khakhong, S., & Sanoamuang, L. (2013). Chromosomal Characteristic of Nile Tilapia (Oreochromis niloticus) from Mitotic and Meiotic Cell Division by T-Lymphocyte Cell Culture. Cytologia, 78(1): 9-14. https://doi.org/10.1508/cytologia.78.9 Add to Citavi project by DOI
• Yamazaki, F., Onazato, H., & Arai, K. (1981). The chopping method for obtaining permanent chromosome preparation from embryos of teleost fishes. Bulletin of the Japanese Society of Scientific Fisheries, 47(7): 963. https://doi.org/10.2331/suisan.47.963 Add to Citavi project by DOI