Expression of stem-cell pluripotency markers in amniotic fluid cells

Abstract

Aim: As being a heterogenous population but having different pools of self-renewing cells, amniotic fluid cells (AFCs) have been considered as promising cell sources for regenerative medicine owing to their multipotency and effective reprogramming capacity. AFCs can more easily and effectively be reprogrammed than terminally differentiated cells to generate induced pluripotent stem cells (iPSCs) by transfectional and chemical strategies. But stem cell dynamics and differentiation capacity of AFCs into multiple lineages were poorly understood. The aim of this study was to determine the expression and stem cell characteristics of stem cell pluripotency markers in amniotic fluid cells. Methods: In this study, the expression profile of pluripotency markers in AFCs taken from 17 patients were investigated by conventional RT-PCR by qualitatively. Results: The results showed that all the pluripotency markers (OCT3/OCT4, SOX2, KLF4, MYC, KIT, NANOG, DPPA3, DPPA5, FUT4, SALL4) were expressed in AFCs from different patients but UTF1 is shows a non-specific expression. Conclusion: Consequently, our results revealed that AFCs have a unique stem cell profile and pluripotency markers may be impactful when choosing cells for reprogramming and regenerative medicine applications. 

Keywords

• Amniotic fluid cells,pluripotency markers,expression,regenerative medicine

Amniyotik sıvı hücrelerinde kök hücre pluripotensi belirteçlerinin ifadesi

Abstract

Amaç: Amniyotik sıvı farklı seviyelerde kendini yenileyebilen hücre gruplarından oluşmaktadır ve bu hücrelerin kök hücre özellikleri henüz tam olarak karakterize edilmemiştir. Son yıllarda amniyotik sıvı hücreleri (AFC) rejenaratif tıp için umut veren kök hücre kaynağı olarak görülmektedir. AFC’ler transfeksiyonel ve kimyasal stratejiler ile indüklenmiş pluripotent kök hücre (iPSC)’ler oluşturmak için terminal olarak farklılaşmış hücrelerden daha kolay ve etkili bir şekilde yeniden programlanabilirler. Fakat AFC’lerin kök hücre potansiyelleri ve farklı hücre soylarına farklılaşma kapasitesi tam olarak anlaşılamamıştır. Bu çalışmada amniyotik sıvı hücrelerinde kök hücre pluripotensi belirteçlerinin ifadelerinin ve kök hücre karakterlerinin belirlenmesi amaçlanmıştır. Yöntem: Bu çalışmada, 17 amniyon sıvısından elde edilen AFC’lerde pluripotensi belirteçlerinin ifade edilip edilmediği geleneksel RT-PCR ile kalitatif olarak araştırılmıştır. Bulgular: Elde edilen sonuçlar, farklı hastalardan alınan AFC örneklerinde incelenen pluripotensi belirteçlerinden OCT3/OCT4, SOX2, KLF4, MYC, KIT, NANOG, DPPA3, DPPA5, FUT4, SALL4’ün ifade edildiğini, UTF1 için ise non-spesifik ifade olduğunu göstermektedir. Sonuç: Elde edilen bulgular doğrultusunda AFC’nin kendine özgü bir kök hücre profiline sahip olduğu ve pluripotensi belirteçlerinin yeniden programlama ve rejeneratif tıp uygulamaları için hücre seçiminde etkili şekilde kullanılabileceği düşünülmektedir.

Keywords

• Amniyotik sıvı hücresi,pluripotensi belirteci,ekspresyon,rejeneratif tıp

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