Yeni tanı kronik myeloid lösemili hastalarda tiyol/disülfit dengesi ve Sokal prognostik skoru ile ilişkisi

Abstract

Amaç: Kronik myeloid lösemi (KML), bcr-abl füzyon geninin pozitifliği ile karakterize olan myeloproliferatif bir hastalıktır. Oksidatif stres bu hastalarda DNA hasarına yol açarak hastalığın patogenezinde rol oynuyor olabilir. Thiol molekülleri organizmayı oksidatif stresin zararlı etkilerinden korumaktadır. Biz de bu çalışmamızda KML hastalarında thiol/disülfit dengesinin durumunu araştırmayı amaçladık. Yöntem: Toplam 48 hasta ve 80 sağlıklı birey çalışmaya dahil edildi. Alınan venöz kan örnekleri santrifüj edilerek ayrılan serum kısmı -800C’de tüm örnekler çalışılana kadar saklandı. Bu kan örneklerinden native thiol, total thiol, disülfit ve İskemi modifiye albümin (İMA) çalışıldı. Bulgular: Hastaların medyan Sokal skoru 0.78 (0.56-1.37) olarak hesaplandı. Sokal skor kategorilerinde native thiol, total thiol, disülfit/native thiol, disülfit/total thiol, native thiol/total thiol ve İMA düzeyleri arasında anlamlı farklılık tespit edilmiştir. Hasta ve kontrol grubu oksidatif stres ölçümü bakımından karşılaştırıldığında, hastalardaki ölçümler artmış oksidatif stres ile uyumlu bulunmuştur. Sokal skoruna göre yüksek risk grubunda yer alan hastalarda serum İMA düzeylerinin anlamlı derecede daha yüksek olduğu görülmüş ve risk arttıkça İMA düzeylerinde de anlamlı düzeyde artış olduğu görülmüştür (p<0.001). Sonuç: KML hastalarında oksidatif stres parametreleri normal bireylere göre anlamlı derecede daha yüksektir. Özellikle serum İMA düzeyleri, Sokal’a göre yüksek risk hastalarda belirgin yüksek olup gelecekte yeni geliştirilecek prognostik skorlara dahil edilebilir.

Keywords

• Kronik myeloid lösemi
• oksidatif stres
• Sokal skoru

Thiol/disulfide balance in patients with newly diagnosed chronic myeloid leukemia and its relationship with Sokal prognostic score

Abstract

Aim: Chronic myeloid leukemia (CML) is a myeloproliferative disease characterized by the presence of the bcr-abl fusion gene. Oxidative stress may play a role in the pathogenesis of this disease by causing DNA damage. Thiol molecules protect the organism from the harmful effects of oxidative stress. In this study, we aimed to investigate the thiol/disulfide balance in CML patients. Method: A total of 48 patients with CML and 80 healthy individuals were included in the study. The collected venous blood samples were centrifuged and the serum part was stored at -80 0C until all samples were studied. Native thiol, total thiol, disulfide and ischemia modified albumin (IMA) levels were studied from these blood samples. Results: The median Sokal score of the patients was calculated as 0.78 (0.56-1.37). In the Sokal score categories, a significant difference was found between the levels of native thiol, total thiol, disulfide/native thiol, disulfide/total thiol, native thiol/total thiol and IMA. When the patient and control groups were compared in terms of oxidative stress measurement, the measurements in the patients were found to be consistent with increased oxidative stress. Serum IMA levels were found to be significantly higher in patients in the high-risk group according to the Sokal score, and it was observed that IMA levels increased as the risk increased (p<0.001). Conclusion: Oxidative stress parameters are significantly higher in CML patients than in normal individuals. In particular, serum IMA levels are significantly higher in high-risk patients according to Sokal and can be included in future prognostic scores.

Keywords

• Chronic myeloid leukemia
• oxidative stress
• Sokal score

Supporting Institution

none

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