Lösin Enkefalin\'in Kurbağa Sinir Sistemindeki Lokalizasyonu

Year 2009, Volume: 2 Issue: 1, 30 - 40, 01.03.2009

Yusuf Çamlıca Ali Aşkın Nejat Yılmaz

Abstract

AbstractLocalization of Leucine Enkephalin on Frog Nerve System Objective: In this study, it is aimed to determine the immunohistochemical localization of opioid peptide, leucine enkephalin in frog nerve tissues. Method: After dissection of frog (n=6) brain, spinal cord and sciatic nerves were isolated. Indirect immunohistochemical and light microscopy techniques were then applied for the distribution of leucine enkephalin in frog nerve tissues. Result: In frog nerve tissues such as brain, spinal cord immunopositive structures were observed. Accordingly, the perikaryon, eppendymal and glial cells were immünoreactive. In addition, immunopositive staining was observed in all axoplasm of the sciatic nerves, but little of fibroblast and Schwann cells were immunoreactive. It was no observed immunoreactivity difference of intensity between tissues of patterns. Conclusion: These findings show that opioid peptide, leucine enkephalin in the frog nervous system was localized. The distribution of leucine enkephalin in the central nervous system is an evidence of their neurotransmitter or neuromodulator role.

Keywords

leucine enkephalin, frog, immunohistochemistry, nerve tissue

Lösin Enkefalin'in Kurbağa Sinir Sistemindeki Lokalizasyonu

Abstract

Amaç: Bu çalışmada, opioid peptitlerden lösin enkefalin'in kurbağa sinir dokularında immünohistokimyasal lokalizasyonunun tespit edilmesi amaçlandı. Yöntem: Kurbağaların (n=6) diseksiyonuyla beyin, omurilik ve siyatik sinir dokuları izole edildi. İndirekt immünohistokimya yöntemi ve ışık mikroskopi tekniği kullanılarak kurbağa sinir dokularında lösin enkefalin yayılışı belirlendi. Bulgular: Kurbağa beyin ve omurilik dokularında immünopozitif hücreler gözlendi. Buna göre perikaryonlar, ependima ve glial hücreler immünopozitif olarak işaretlendi. Ayrıca, siyatik sinir aksoplazmalarının çoğunda immünoreaktivite saptandı fakat, fibroblast ve Schwann hücrelerinin az bir kısmında immünoreaktivite izlendi. İncelenen deneklerin dokuları arasında immünoreaktivite kuvveti bakımından herhangi bir fark gözlenmedi. Sonuç: Bulgular, kurbağa sinir sisteminde opioid peptitlerden lösin enkefalin'in lokalize olduğuna işaret etmektedir. Bu peptitin merkezi sinir sisteminde yayılış göstermesi, nörotransmitter veya nöromodülatör rolünün olabileceğini göstermektedir.

Keywords

lösin enkefalin, kurbağa, immünohistokimya, sinir doku

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