AbstractThe Comparison of Ion Exchange Chromatography and Immunoturbidimetric Methods of HemoglobinA1c Results Objective: Development of chronic complications of Diabetes Mellitus, a public health problem, is related with long term blood glucose levels of the patient. Today, measurement of blood hemoglobin A1c (HbA1c) levels were used as an indicator of the risk for development of DM related complications and average retrospective glisemic status. There exist numerous different analysis methods for the determination of blood HbA1c levels in routine test laboratories. Method: The relationship between the blood HbA1c data obtained with both immunoturbidimetric method by Cobas Integra 800 and ion exchange chromatography by Agilent 1100 HPLC system was evaluated. Results: The mean±standart deviation of blood HbA1c levels obtained by both Cobas Integra 800 and Agilent 1100 HPLC system are 6.06±1.83 and 6.29±1.59; respectively. Among the data obtained by these two methods, the intraclass correlation coefficient and concordance correlation coefficient values were found as 0.9468 (95% CI=0.9223-0.9638); 0.9382 (95% CI=0.9128-0.9563) (p<0.0001), respectively. Furthermore, it was determined that the data obtained by these two methods are in good agreements with the Bland&Altman Plot (r=-0.042, p=0.672). Conclusion: Based on the data obtained in this study, it can be concluded that these two different measurement tools are compatible with each other. However, the most advantageous method should be selected for operation, considering the needs and priorities of the test laboratory as both methods possess different advantageous and disadvantageous.
Türkçe Özet:Amaç: Bir halk sağlığı problemi olan Diabetes Mellitus\'un kronik komplikasyonlarının oluşumu, hastanın uzun dönem kan glukoz düzeyi ile ilişkilidir. Günümüzde geçmişe yönelik ortalama glisemik durumun ve komplikasyon gelişme riskinin göstergesi olarak kan HemoglobinA1c (HbA1c) düzeylerinin ölçümü kullanılmaktadır. Rutin laboratuarlarda sık çalışılan testler arasına giren HbA1c ölçümü için bugün kullanılan birçok farklı analiz yöntemi vardır. Yöntem: Cobas Integra800 otoanalizöründe immünotürbidimetrik yöntemle çalıştığımız HbA1c sonuçları ile Agilent 1100 HPLC sistemi iyon değişim kromatografisi yöntemi ile elde ettiğimiz HbA1c sonuçları arasındaki ilişki değerlendirildi. Bulgular: Cobas Integra 800 ve Agilent 1100 HPLC sistemi ile ölçülen HbA1c değerleri sırasıyla 6.06± 1.83 ve 6.29±1.59 olarak bulunmuştur. İki yöntem sonuçları arasında, sınıf içi korelasyon katsayısı 0.9468 (%95 GA=0.9223-0.9638) (p<0.0001) ve concordance korelasyon katsayısı 0.9382 ((%95 GA=0.9128-0.9563) (p<0.0001) olarak bulunmuştur. Ayrıca Bland&Altman grafiği ile (r=-0.042, p=0.672) iki yöntem sonuçlarının uyum içinde olduğu belirlenmiştir. Sonuç: Bu sonuçlara göre iki farklı ölçme aracının birbirleriyle uyumlu olduğu, bununla beraber her iki yöntemin farklı avantaj ve dezavantajlarının olması nedeniyle, laboratuarın ihtiyacı ve önceliklerine göre iki yöntem arasında seçim yapılabileceği sonucuna varıldı.
Primary Language | Turkish |
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Journal Section | Articles |
Authors | |
Publication Date | June 1, 2009 |
Submission Date | June 13, 2014 |
Published in Issue | Year 2009 Volume: 2 Issue: 2 |
MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.
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