Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon

Karwan Taalat; Muhammad Arshad Javed; Fahrul Zaman Huyop; Yilmaz Kaya

doi:10.51354/mjen.839516

EN

Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon

Abstract

Current study is to establish a basic plant tissue culture of Nicotiana tabacum TAPM 26 and test the plant tissue on resistancies against 2,2 DCP an active ingredient in herbicide-Dalapon. During micropropagation, the surface sterilization method was ascertained on seeds of tobacco. HgCl2 was used to disinfect tobacco seeds at different concentrations (0.05 gL-1, 0.2 gL-1, 0.5 gL-1 and 1.0 gL-1) within three minutes. About 70% seeds were survived when exposed to 0.05 gL-1 of HgCl2, whereas, no seeds were germinated when sterilized at concentrations above 0.05 gL-1 of HgCl2. To optimize an efficient protocol of shoots and callus formation during in vitro regeneration, explant types and plant growth were studied. Growth regulators NAA (0.1 mgL-1, 0.2 mgL-1, 0.5 mgL-1, 1.0 mgL-1 and 2.0 mgL-1) and BAP (1.0 mgL-1, 2.0 mgL-1, 3.0 mgL-1 and 4.0 mgL-1) were used. The explants types were one month old leaves and two weeks old cotyledons. The maximum numbers of shoots per explants were obtained from cotyledon with combination 0.1 mgL-1 NAA and 1.0 mgL-1 BAP. The highest callus fresh weight was achieved when NAA 0.5 mgL-1 with BAP 1.0 mgL-1 after four weeks. Thus, the highest number of shoots produced per explants from leaves culture on the MS media containing 0.2 mgL-1 NAA and 4.0 mgL-1 BAP. The best callus fresh weight was obtained with combination of 1.0 mgL-1 NAA and 1.0 mgL-1 BAP by using leaves explant. Finally, Dalapon (5 gL-1, 10 gL-1, 15 gL-1 and 20 gL-1) were applied onto leaves and cotyledon cultures of N. tabacum to check on the minimum concentration of inhibition. The minimum concentration of inhibition of leaves and cotyledon cultures of N. tabacum was at 5 gL-1 of 2,2DCP but not at 10 gL-1, 15 gL-1 and 20 gL-1. This investigation will shed alight for future studies on transgenic tobacco resistant against Dalapon

Keywords

Seeds sterilization, NAA, BAP, callus formation, Shoot formation, Dalapon

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Karwan Taalat
0000-0003-4195-8699
Malaysia

Muhammad Arshad Javed
0000-0001-9629-4215
Malaysia

Fahrul Zaman Huyop
0000-0003-3978-4087
Malaysia

Yilmaz Kaya ^*
0000-0003-1506-7913
Kyrgyzstan

Publication Date

April 30, 2021

Submission Date

December 12, 2020

Acceptance Date

March 9, 2021

Published in Issue

Year 2021 Volume: 9 Number: Special 1

DOI

https://doi.org/10.51354/mjen.839516

IZ

https://izlik.org/JA69CZ84XF

APA

Taalat, K., Javed, M. A., Huyop, F. Z., & Kaya, Y. (2021). Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MANAS Journal of Engineering, 9(Special 1), 35-42. https://doi.org/10.51354/mjen.839516

AMA

1.Taalat K, Javed MA, Huyop FZ, Kaya Y. Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MJEN. 2021;9(Special 1):35-42. doi:10.51354/mjen.839516

Chicago

Taalat, Karwan, Muhammad Arshad Javed, Fahrul Zaman Huyop, and Yilmaz Kaya. 2021. “Plant Tissue Culture of Nicotiana Tabacum Cv. TAPM 26 and Its Minimum Inhibition Against Herbicide-Dalapon”. MANAS Journal of Engineering 9 (Special 1): 35-42. https://doi.org/10.51354/mjen.839516.

EndNote

Taalat K, Javed MA, Huyop FZ, Kaya Y (April 1, 2021) Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MANAS Journal of Engineering 9 Special 1 35–42.

IEEE

[1]K. Taalat, M. A. Javed, F. Z. Huyop, and Y. Kaya, “Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon”, MJEN, vol. 9, no. Special 1, pp. 35–42, Apr. 2021, doi: 10.51354/mjen.839516.

ISNAD

Taalat, Karwan - Javed, Muhammad Arshad - Huyop, Fahrul Zaman - Kaya, Yilmaz. “Plant Tissue Culture of Nicotiana Tabacum Cv. TAPM 26 and Its Minimum Inhibition Against Herbicide-Dalapon”. MANAS Journal of Engineering 9/Special 1 (April 1, 2021): 35-42. https://doi.org/10.51354/mjen.839516.

JAMA

1.Taalat K, Javed MA, Huyop FZ, Kaya Y. Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MJEN. 2021;9:35–42.

MLA

Taalat, Karwan, et al. “Plant Tissue Culture of Nicotiana Tabacum Cv. TAPM 26 and Its Minimum Inhibition Against Herbicide-Dalapon”. MANAS Journal of Engineering, vol. 9, no. Special 1, Apr. 2021, pp. 35-42, doi:10.51354/mjen.839516.

Vancouver

1.Karwan Taalat, Muhammad Arshad Javed, Fahrul Zaman Huyop, Yilmaz Kaya. Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MJEN. 2021 Apr. 1;9(Special 1):35-42. doi:10.51354/mjen.839516