Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism

Mehmet Enes Arslan

doi:10.51354/mjen.848282

EN

Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism

Abstract

This study aimed to investigate the anticarcinogenic and genotoxic damage potentials of malic acid on human fibroblast cells (HDFa) and glioblastoma (U87-MG) cell lines. MTT cell viability and LDH release assays were performed to understand cytotoxic features of malic acid on different cell lines. Also, Hoechst 33258 fluorescent staining was used to monitor nuclear abnormalities including micronucleus, lobbed, and notched structures. Furthermore, cellular death mechanisms behind the malic acid application were investigated via the use of flow cytometry analysis. According to cell viability analysis, malic acid showed a greater effect on U87-MG compared to HDFa cell line in terms of cytotoxicity. Similarly, chromosomal integrity assay put forth a higher number of nuclear abnormalities in U87-MG cells when compared to HDFa cell lines, and aberrations were analyzed to amplify when malic acid concentration increased. Finally, flow cytometry analysis demonstrated higher necrotic cell death in U87-MG cells than HDFa cell line. On the other hand, apoptotic cell death was the main cytotoxic mechanism against malic acid exposure in the HDFa cell line. In light of these results, it can be concluded that in higher concentrations, malic acid has an anticarcinogenic effect on glioblastoma cells via the necrotic pathway, and it also shows apoptotic properties on the fibroblast cell line. When mutagenic properties are compared, it could be understood that malic acid had a greater impact on glioblastoma cells.

Keywords

malic acid, glioblastoma, in vitro, necrosis, DNA mutations

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Mehmet Enes Arslan ^*
0000-0002-1600-2305
Türkiye

Publication Date

June 30, 2021

Submission Date

December 28, 2020

Acceptance Date

March 9, 2021

Published in Issue

Year 2021 Volume: 9 Number: 1

DOI

https://doi.org/10.51354/mjen.848282

IZ

https://izlik.org/JA65BU38CT

APA

Arslan, M. E. (2021). Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism. MANAS Journal of Engineering, 9(1), 22-29. https://doi.org/10.51354/mjen.848282

AMA

1.Arslan ME. Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism. MJEN. 2021;9(1):22-29. doi:10.51354/mjen.848282

Chicago

Arslan, Mehmet Enes. 2021. “Anticarcinogenic Properties of Malic Acid on Glioblastoma Cell Line through Necrotic Cell Death Mechanism”. MANAS Journal of Engineering 9 (1): 22-29. https://doi.org/10.51354/mjen.848282.

EndNote

Arslan ME (June 1, 2021) Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism. MANAS Journal of Engineering 9 1 22–29.

IEEE

[1]M. E. Arslan, “Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism”, MJEN, vol. 9, no. 1, pp. 22–29, June 2021, doi: 10.51354/mjen.848282.

ISNAD

Arslan, Mehmet Enes. “Anticarcinogenic Properties of Malic Acid on Glioblastoma Cell Line through Necrotic Cell Death Mechanism”. MANAS Journal of Engineering 9/1 (June 1, 2021): 22-29. https://doi.org/10.51354/mjen.848282.

JAMA

1.Arslan ME. Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism. MJEN. 2021;9:22–29.

MLA

Arslan, Mehmet Enes. “Anticarcinogenic Properties of Malic Acid on Glioblastoma Cell Line through Necrotic Cell Death Mechanism”. MANAS Journal of Engineering, vol. 9, no. 1, June 2021, pp. 22-29, doi:10.51354/mjen.848282.

Vancouver

1.Mehmet Enes Arslan. Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism. MJEN. 2021 Jun. 1;9(1):22-9. doi:10.51354/mjen.848282