Determination of Amino Acid Composition of Cow’s Milk by Liquid Chromatography Using Precolumn Derivatization

Year 2017, Volume: 5 Issue: 3, 25 - 34, 01.12.2017

A. Mazhitova , A. Kulmyrzaev

Abstract

The application of the procedure of pre-column derivatization with diethyl
ethoxymethylenemalonate in the determination of the amino acid content of milk by reversedphase
chromatography with diode-matrix detection was investigated. The influence of the pH on
the response factor was established. The conditions of modification of amino acids during
sample preparation procedure were optimized. A complete separation of 18 amino acids was
achieved. The most abundant amino acid in cow's milk was glutamic acid, the concentration of
which twice that of leucine and lysine, and three times greater than aspartic acid

Keywords

Аминокислота, белки, молоко, хроматография

References

• 1. Baranovsky A. Yu. Dietology. 4 th ed. Publishing house "Peter". 2013. (in Russian)
• 2. Böcker Yu. Chromatography. Instrumental analytics: methods of chromatography and capillary electrophoresis. Litres. (2017). (in Russian)
• 3. Kharitonov Yu. Ya., Dzhabarov D. N. and Grigorieva V. Yu. Analytical chemistry. Quantitative analysis, physicochemical methods of analysis: practical: textbook: [for universities on specialty 060301.65 "Pharmacy"]. GEOTAR-Media. (2012). (in Russian)
• 4. Rudenko A. O., Kartsova L. A. and Snarskii S. I. "Determination of the most important amino acids in complex objects of biological origin by reversed-phase HPLC with obtaining phenylthiohydantoins of amino acids". Sorption and chromatographic processes, 10 (2), 223- 230. (2010). (in Russian)
• 5. Bosch L., Alegría A., Farré R. Application of the 6-aminoquinolyl-N-hydroxysccinimidyl carbamate (AQC) reagent to the RP-HPLC determination of amino acids in infant foods. Journal of Chromatography B. 831(1) (2006): 176-83.
• 6. Davis T.A., Nguyen H.V., Garcia-Bravo R., Fiorotto M.L., Jackson E.M., Reeds P.J.. Amino acid composition of the milk of some mammalian species changes with stage of lactation. British journal of nutrition. 72(06) (1994): 845-53.
• 7. Marino R., Iammarino M., Santillo A., Muscarella M., Caroprese M., Albenzio M. Technical note: Rapid method for determination of amino acids in milk. Journal of dairy science. 93(6) (2010):2367-70.
• 8. Rubio-Barroso S., Santos-Delgado M.J., Martin-Olivar C., Polo-Díez L.M. Indirect chiral HPLC determination and fluorimetric detection of D-amino acids in milk and oyster samples. Journal of dairy science. 89(1) (2006):82-9.
• 9. Rebane R., Herodes K. A sensitive method for free amino acids analysis by liquid chromatography with ultraviolet and mass spectrometric detection using precolumn derivatization with diethyl ethoxymethylenemalonate: Application to the honey analysis. Analytica chimica acta. 672(1) (2010): 79-84.
• 10. Barashkin M. I. "Amino acid composition of milk of cows of black and motley type of breed "Uralsky", depending on lactation phase". Agrarian Bulletin of the Urals 8 (100) (2012): 22- 24. (in Russian)
• 11. Gorbatova K. K., and Gunkova P. I. Biochemistry of milk and dairy products. GIORD. (2010). (in Russian)
• 12. Rebane R, Oldekop ML, Herodes K. Comparison of amino acid derivatization reagents for LC–ESI-MS analysis. Introducing a novel phosphazene-based derivatization reagent. Journal of Chromatography B. 904(2012):99-106.
• 13. Hermos n I, Chic n RM, Cabezudo MD. Free amino acid composition and botanical origin of honey. Food Chemistry. 83(2) (2003): 263-8.
• 14. Lebedev A. Mass spectrometry for the analysis of environmental objects. Litres. (2017). (in Russian).
• 15. Mazhitova AT, Kulmyrzaev AA. Determination of amino acid profile of mare milk produced in the highlands of the Kyrgyz Republic during the milking season. Journal of dairy science. 99(4) (2016): 2480-7.
• 16. Shapovalova E. N. and Pirogov A. V. Chromatographic methods of analysis. Methodical manual for a special course. Moscow: Moscow State University Press. (2007). (in Russian)
• 17. Shabir GA. Validation of high-performance liquid chromatography methods for pharmaceutical analysis: Understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International Conference on Harmonization. Journal of chromatography A. 987(1) (2003): 57-66.
• 18. Csap J, Salamon S, L ki K, Csap -Kiss Z. Composition of mare’s colostrum and milk II. Protein content, amino acid composition and contents of macro-and micro-elements. Acta Univ. Sapient. Ser. Aliment.2(1) (2009): 133-48.
• 19. Skurikhin I. M. and Volgarev M. N. Chemical composition of food products. Ripol Classic, (1987) (in Russian)