Genetic Characterization of Bay Laurel (Laurus nobilis L.) Populations Using Microsatellite Markers and Flow Cytometry

Year 2018, Volume: 23 Issue: 2, 242 - 253, 25.12.2018

Mehmet Çağlar Bulut Canan Yüksel Özmen Ali Ergül Filiz Ayanoğlu

Abstract

Turkey is one of the few countries that exports the highest quality bay laurel leaf and covers about 90% of the world bay laurel leaf trade. In this study, 95 bay laurel genotypes selected from flora of Hatay province for their superior characteristics were used. Selected genotypes were genetically characterized by 6 SSR markers and the DNA contents were determined by Flow Cytometry. No polyploidy was determined as a result of flow cytometry analysis and 2C DNA values were observed between 5.91 and 6.36 pg. As a result of the SSR analysis, a total of 82 alleles were obtained with a mean of 16.4 of 5 polymorphic loci, while LnD106 loci were observed monomorphic. The highest number of alleles (24 bp) was observed in the LnA2 locus. Generally, a low similarity is determined among the genotypes. The highest genetic similarity was seen in E6 and O6 genotypes with 80%. This situation revealed the importance of genetic diversity in Hatay bay laurel population. The results are important as regard to reveal and protect the genetic diversity of bay laurel existence in Hatay.

Keywords

Genetic diversity, L. nobilis, DNA content, SSR

Defne (Laurus nobilis L.) Populasyonlarının Mikrosatellit Markörler ve Flow Sitometri ile Genetik Karakterizasyonu

Abstract

Türkiye, yüksek kaliteli defne yaprağı ihraç eden birkaç
ülkeden biridir ve dünya defne yaprağı ticaretinin yaklaşık% 90'ını Türkiye
yapmaktadır. Bu çalışmada üstün özellikleri nedeniyle Hatay florasından seçilen
95 adet defne genotipi kullanılmıştır. Seçilen genotipler, genetik olarak 6 SSR
markörü ile karakterize edilmiş ve DNA içerikleri Flow Cytometry ile
belirlenmiştir. Flow sitometri analizi sonucunda poliploidi saptanmamış ve 2C
DNA değerleri 5.91 ile 6.36 pg arasında gözlenmiştir. SSR analizi sonucunda, 5
polimorfik lokusta ortalama 16.4 ile toplam 82 allel elde edilirken, LnD106
lokusu monomorfik olarak gözlenmiştir. En yüksek allel sayısı (24 bp) LnA2
loküsünde gözlenmiştir. Genel olarak, genotipler arasında benzerlik düşük
olmuştur. En yüksek genetik benzerlik oranı % 80 ile E6 ve O6 genotiplerinde
görülmüştür Bu durum Hatay defne populasyonundaki genetik çeşitliliğin önemini
ortaya çıkarmıştır. Sonuçlar, Hatay'da defne varlığının genetik çeşitliliğini
ortaya koymak ve korumak açısından önemlidir. 

Keywords

Genetik çeşitlilik, L. nobilis, DNA içeriği

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