The role of -1984A> G adrenomedullin gene polymorphism in tubal ectopic pregnancy

Abstract

Objective: Tubal ectopic pregnancy is a health issue that can cause maternal death in first trimester and our knowledge about its pathogenesis is limited. Adrenomedullin (ADM) is responsible from regulation of ciliary motility in fallopian tubes. Its expression is known to be reduced in tubal tissue in ectopic pregnancy. In this study, it was aimed to investigate the relationship between -1984A>G functional polymorphism affecting the expression level of the ADM gene and tubal ectopic pregnancies. Material and Methods: This prospective case-control study consisted of 64 women. Peripheral blood samples were obtained from 31 women diagnosed with tubal pregnancy (the study group) and 33 fertile women without a history of ectopic pregnancy (the control group). Genomic DNA was extracted from peripheral blood. The frequency of genotypes and alleles of â€"1984A>G ADM (rs3814700) gene polymorphism was examined by polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method. Results: For -1984A>G polymorphism the study group had an A allele frequency of 90% and a G allele frequency of 10%; the control group had an A allele frequency of 91.7% and a G allele frequency of 7.3% (p=0.75). AA genotype frequency was 80% and AG allele frequency was 20% in the study group while they were 83.3% and 16.7%, respectively, in the control group (p=0.73). There were no significant differences between the study and control groups with respect to allele and genotype frequencies. Conclusion: This study found no significant relationship between tubal ectopic pregnancy and -1984A>G ADM gene polymorphism. Further studies are needed to explore other factors that affect ADM expression.

Keywords

• Adrenomedullin, Tubal ectopic pregnancy, Genetic polymorphism

References

1. Fazeli A, Affara NA, Hubank M, Holt WV. Sperm
2. induced modification of the oviductal gene expression
3. profile after natural insemination in mice. Biology of
4. Cheung BM, Ong KL, Tso AW, Leung RY, Cherny SS,
5. Sham PC, et al. Plasma adrenomedullin level is related to
6. a single nucleotide polymorphism in the adrenomedullin
7. gene. European journal of endocrinology / European
8. Federation of Endocrine Societies. 2011;165(4):571-7.

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