Adrenomedullin has effects on hypoxia-inducible factor 1-alpha in hypoxia in several organs of female rats

Abstract

Objective: Vascular dysfunction induces impaired O2 delivery and hypoxia, and adrenomedullin (AdM) have a role in hypoxia and angiogenesis. The reaction of the organism exposed to hypoxia and the effect of AdM over HIF-1α has been investigated in this study. Material and Methods: Female rats were divided into 4 groups (control, hypoxia, control + AdM and hypoxia + AdM). Hypoxia groups were provided hypoxia containing 10% oxygen and 90% nitrogen for 1 week. AdM treatment groups were injected 1.25 nmol/kg AdM for 4 days intraperitoneally. AdM and HIF-1α levels were measured in heart, lung, and kidney tissues with ELISA. Results: AdM levels increased in hypoxia + AdM group than hypoxia group, however, was not increased in control + AdM group when compared with the control group in heart tissue. Contrarily, AdM decreased in control + AdM group in the kidney, while increased in control + AdM, and hypoxia + AdM groups in the lung when compared to the control group. The HIF-1α level was higher in control + AdM group in the kidney, and hypoxia + AdM group in the lung. HIF-1α levels in heart tissue were decreased in hypoxia group when compared to the control group. Conclusion: Tissues respond to hypoxic conditions at different times, and at distinct levels. AdM may be used to induce HIF-1α therapeutically before ischemic conditions.

Keywords

• Adrenomedullin,hypoxia,hypoxia-inducible factor 1α,angiogenesis

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