The effects of 2-aminoethyl diphenylborinate on L-Arginine induced acute pancreatitis in the rats

Year 2015, Volume: 2 Issue: 6, 352 - 7, 04.09.2015

Murat Yildar , Murat Basbug , Omer Ozkan , Faruk Cavdar İsmail Yaman Hasan Aksit Musa Ozyigit Figen Aslan Hayrullah Derici

Abstract

Intraduction: The aim of the study is to investigate the protective effect of 2-aminoethyl diphenylborinate  on an acute pancreatitis model through an experimental study.

Materials and Methods: 30 Spraque-Dawley male rats were randomly divided into three groups:  Sham ,  Pancreatitis and  Pancreatitis + 2-APB. Pancreatitis was induced by L-arginine administration. The therapeutic agent 2-APB was performed i.v. at a dose of 2 mg/kg 10 min before pancreatitis induction. From blood samples, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity, tumor necrosis factor alpha, interleukin-6, aspartate aminotransferase , alanine aminotransferase and creatinine levels were measured and the rats were sacrificed subsequently. Tissue samples were evaluated histopathologically. TUNEL staining method was used to visualize apoptotic cells.

Results: 2-APB significantly reduced serum MDA and creatinine levels in pancreatitis + 2-APB group. Unfortunately, SOD levels reduced significantly, too. Edema and hemorrhage in pancreatic tissue were lower, necrosis and fibrosis were higher in the 2-APB administered group. Additionally, in 2-APB given group, it was found that vacuolisation, epithelial desquamation, and congestion reduced in renal tubular epithelial. The number of apoptotic cells did not change in the pancreatic tissue in TUNEL staining.

Conclusions: 2-APB reduces renal damage caused by acute pancreatitis. However, protective effect has not been on pancreatic tissue with 2-APB administered group. Although 2-APB, which was shown to prevent the degradation of kidney functions due to pancreatitis, do not minimize the pancreas tissue damage, it can improve the prognosis of pancreatitis by reducing the damage of distant organs 

Keywords

2-aminoethyl diphenylborinate, acute pancreatitis, kidney, oxidative stress, antioxidants

The effects of 2-aminoethyl diphenylborinate on L-Arginine induced acute pancreatitis in the rats

Abstract

References

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