CYTOTOXICITY AND WOUND HEALING CAPACITY OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES

Year 2022, , 63 - 68, 28.06.2022

Tuba Baygar , Nurdan Saraç , Aysel Uğur

https://doi.org/10.22531/muglajsci.1087581

Cited By: 1

Abstract

Metallic nanoparticles are interesting areas of research due to their unique properties which can be advantageous for producing smart products. Silver nanoparticles (AgNPs) are remarkably used in pharmaceutical industry because of their strong biological activities. The aim of the present study was to investigate the cytotoxicity and wound healing capacity of the biologically prepared silver nanoparticles via green synthesis route. Cytotoxicity of the biogenic AgNPs was determined by MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide) assay against L929 fibroblast cell line. Wound healing properties of the AgNPs were evaluated using in vitro-scratch wound healing assay using 3T3 fibroblast cell line. Biosynthesized AgNPs inhibited the propagation of fibroblasts at a half maximal inhibitory concentration (IC₅₀) of 23.507 μg/mL after 24 h incubation. In vitro wound healing assay also revealed that the biogenic AgNPs stimulated the 3T3 fibroblasts' cell proliferation. It can be suggested that biologically synthesized AgNPs can be used effectively for biomedical applications such as wound dressing materials.

Keywords

silver nanoparticle, green synthesis, cytotoxicity, wound healing

Thanks

Cytotoxicity analysis was performed at AUBIBAM (Anadolu University Medicinal Plants, Drugs and Scientific Research Center), Anadolu University, Eskişehir, Türkiye.

BİYOLOJİK OLARAK SENTEZLENMİŞ GÜMÜŞ NANOPARTİKÜLLERİN SİTOTOKSİSİTESİ VE YARA İYİLEŞTİRME KAPASİTESİ

Abstract

References

• Mathur, P., Jha, S., Ramteke, S., Jain, N. K., “Pharmaceutical aspects of silver nanoparticles”, Artificial cells, nanomedicine, and biotechnology, 46(sup1), 115-126, 2018.
• Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M., Mozafari, M. R., “Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties”, International journal of nanomedicine, 13, 8013-8024, 2018.
• Dhand, V., Soumya, L., Bharadwaj, S., Chakra, S., Bhatt, D., Sreedhar, B., “Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity”, Materials Science and Engineering: C, 58, 36-43, 2016.
• Palaniappan, P., Sathishkumar, G., Sankar, R., “Fabrication of nano-silver particles using Cymodocea serrulata and its cytotoxicity effect against human lung cancer A549 cells line”, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 138, 885-890, 2015.
• El-Sonbaty, S. M., “Fungus-mediated synthesis of silver nanoparticles and evaluation of antitumor activity”, Cancer Nanotechnology, 4(4), 73-79, 2013.
• Nadagouda, M. N., Iyanna, N., Lalley, J., Han, C., Dionysiou, D. D., Varma, R. S., “Synthesis of silver and gold nanoparticles using antioxidants from blackberry, blueberry, pomegranate, and turmeric extracts”, ACS Sustainable Chemistry & Engineering, 2(7), 1717-1723, 2014.
• Iravani, S., “Green synthesis of metal nanoparticles using plants”, Green Chemistry, 13(10), 2638-2650, 2011.
• Roopan, S. M., Madhumitha, G., Rahuman, A. A., Kamaraj, C., Bharathi, A., Surendra, T. V., “Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity”, Industrial Crops and Products, 43, 631-635. 2013.
• Patra, J. K., Baek, K. H., “Green nanobiotechnology: factors affecting synthesis and characterization techniques”, Journal of Nanomaterials, 2014, 1-12, 2014.
• Aziz, N., Faraz, M., Pandey, R., Shakir, M., Fatma, T., Varma, A., ... & Prasad, R., “Facile algae-derived route to biogenic silver nanoparticles: synthesis, antibacterial, and photocatalytic properties”, Langmuir, 31(42), 11605-11612, 2015.
• Yazdi, M. E. T., Khara, J., Housaindokht, M. R., Sadeghnia, H. R., Bahabadi, S. E., Amiri, M. S., ... & Darroudi, M., “Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties”, IET nanobiotechnology, 13(2), 189-192, 2019.
• Pugazhenthiran, N., Anandan, S., Kathiravan, G., Udaya Prakash, N. K., Crawford, S., & Ashokkumar, M., “Microbial synthesis of silver nanoparticles by Bacillus sp.”, Journal of Nanoparticle Research, 11(7), 1811-1815, 2009.
• Dhoondia, Z. H., Chakraborty, H., “Lactobacillus mediated synthesis of silver oxide nanoparticles”, Nanomaterials and nanotechnology, 2, 15, 1-7, 2012.
• Składanowski, M., Golinska, P., Rudnicka, K., Dahm, H., Rai, M., “Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles”, Medical microbiology and immunology, 205(6), 603-613, 2016.
• Zhao, X., Xu, X., Ai, C., Yan, L., Jiang, C., & Shi, J., “Advantages of silver nanoparticles synthesized by microorganisms in antibacterial activity”, In Green Synthesis of Silver Nanomaterials, Elsevier, Netherlands, 2022
• Durán, N., Seabra, A. B., “Metallic oxide nanoparticles: state of the art in biogenic syntheses and their mechanisms”, Applied Microbiology and Biotechnology, 95(2), 275-288, 2012.
• Capeness, M. J., Echavarri-Bravo, V., & Horsfall, L. E., “Production of biogenic nanoparticles for the reduction of 4-nitrophenol and oxidative Laccase-Like reactions”, Frontiers in Microbiology, 10, 997, 2019.
• Guilger-Casagrande, M., Lima, R. D., “Synthesis of silver nanoparticles mediated by fungi: a review”, Frontiers in Bioengineering and Biotechnology, 7, 287, 2019.
• Anandaradje, A., Meyappan, V., Kumar, I., & Sakthivel, N., “Microbial synthesis of silver nanoparticles and their biological potential”, In Nanoparticles in medicine, Springer, Singapore, 2020.
• Wypij, M., Czarnecka, J., Świecimska, M., Dahm, H., Rai, M., Golinska, P., “Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain”, World Journal of Microbiology and Biotechnology, 34(2), 1-13, 2018.
• Procópio, R. E. D. L., Silva, I. R. D., Martins, M. K., Azevedo, J. L. D., Araújo, J. M. D., “Antibiotics produced by Streptomyces”, Brazilian Journal of Infectious Diseases, 16(5), 466-471, 2012.
• Berdy, J., “Bioactive microbial metabolites”, The Journal of antibiotics, 58(1), 1-26. 2005.
• Newman, D. J., Cragg, G. M., “Natural products as sources of new drugs over the last 25 years”, Journal of natural products, 70(3), 461-477, 2007.
• Olano, C., Méndez, C., Salas, J. A., “Antitumor compounds from actinomycetes: from gene clusters to new derivatives by combinatorial biosynthesis”, Natural product reports, 26(5), 628-660, 2009.
• Shanmugaiah, V., Harikrishnan, H., Al-Harbi, N. S., Shine, K., Khaled, J. M., Balasubramanian, N., Kumar, R. S., “Facile synthesis of silver nanoparticles using Streptomyces sp. VSMGT1014 and their antimicrobial efficiency”, Digest Joyrnal of Nanomaterials and Biostructures, 10(1), 179-187, 2015.
• Wypij, M., Czarnecka, J., Świecimska, M., Dahm, H., Rai, M., Golinska, P., “Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain”, World Journal of Microbiology and Biotechnology, 34(2), 1-13, 2018.
• Paterlini, P., Rodríguez, C., Ledesma, A., Pereyra, J., Costa, J. S. D., Álvarez, A., Romero, C. M., “Characterization of biosynthesized silver nanoparticles from Streptomyces aqueous extract and evaluation of surface-capping proteins involved in the process”, Nano-Structures & Nano-Objects, 26, 100755, 2021.
• Baygar, T., Ugur, A. “Biosynthesis of silver nanoparticles by Streptomyces griseorubens isolated from soil and their antioxidant activity”, IET nanobiotechnology, 11(3), 286-291, 2017.
• Baygar, T., Ugur, A., “In vitro evaluation of antimicrobial and antibiofilm potentials of silver nanoparticles biosynthesised by Streptomyces griseorubens”, IET Nanobiotechnology, 11(6), 677-681, 2017.
• Sarac, N., Baygar, T., Ugur, A., “In vitro mutagenic and anti-mutagenic properties of green synthesised silver nanoparticles”, IET Nanobiotechnology, 12(2), 230-233, 2018.
• Mosmann, T., “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays”, Journal of immunological methods, 65(1-2), 55-63, 1983.
• Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols; 2(2): 329-333, 2007.
• Rodriguez, L. G., Wu, X., Guan, J. L., Wound-healing assay. In Cell migration, Humana Press, USA, 2005.
• Jaganathan, S. K., Balaji, A., Vellayappan, M. V., Subramanian, A. P., John, A. A., Asokan, M. K., Supriyanto, E., “Radiation-induced surface modification of polymers for biomaterial application”, Journal of Materials Science, 50(5), 2007-2018, 2015.
• Dehghanizade, S., Arasteh, J., Mirzaie, A., “Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities”, Artificial Cells, Nanomedicine, and Biotechnology, 46(1), 160-168, 2018.
• Carlson, C., Hussain, S. M., Schrand, A. M., K. Braydich-Stolle, L., Hess, K. L., Jones, R. L., Schlager, J. J., “Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species”, The journal of physical chemistry B, 112(43), 13608-13619, 2008.
• Zhao, W., Lu, X., Yuan, Y., Liu, C., Yang, B., Hong, H., ... & Zeng, F., “Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines”, International journal of nanomedicine, 6, 1569-1577, 2011.
• Chowdhury, N. R., MacGregor-Ramiasa, M., Zilm, P., Majewski, P., Vasilev, K., “’Chocolate’silver nanoparticles: Synthesis, antibacterial activity and cytotoxicity”, Journal of colloid and interface science, 482, 151-158, 2016.
• Wei, L., Tang, J., Zhang, Z., Chen, Y., Zhou, G., Xi, T., Investigation of the cytotoxicity mechanism of silver nanoparticles in vitro”, Biomedical Materials, 5(4), 044103, 2010.
• Jahan, I., Erci, F., Isildak, I., “Rapid green synthesis of non-cytotoxic silver nanoparticles using aqueous extracts of 'Golden Delicious' apple pulp and cumin seeds with antibacterial and antioxidant activity”, SN Applied Sciences, 3(1), 1-14, 2021.
• Xia, T., Kovochich, M., Liong, M., Zink, J. I., Nel, A. E., “Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways”, ACS nano, 2(1), 85-96, 2008.
• Moadab, S., Aharı, H., Shahbazzadeh, D., Motalebı, A. A., Anvar, A. A., Rahmanıa, J., Shokrgozar, M. R., “Toxicity study of nanosilver (Nanocid®) on osteoblast cancer cell line”, International Nano Letters, 1(1), 11-16, 2011.
• de Lima, R., Seabra, A. B., Durán, N., “Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles”, Journal of Applied Toxicology, 32(11), 867-879, 2012.
• Fox, L. T., Mazumder, A., Dwivedi, A., Gerber, M., Du Plessis, J., Hamman, J. H., “In vitro wound healing and cytotoxic activity of the gel and whole-leaf materials from selected aloe species”, Journal of ethnopharmacology, 200, 1-7, 2017.
• Hu, X., Kandasamy Saravanakumar, T. J., Wang, M. H., “Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus Talaromyces purpureogenus”, International Journal of Nanomedicine, 14, 3427, 2019.
• Kumar, S. S. D., Houreld, N. N., Kroukamp, E. M., Abrahamse, H., “Cellular imaging and bactericidal mechanism of green-synthesized silver nanoparticles against human pathogenic bacteria”, Journal of Photochemistry and Photobiology B: Biology, 178, 259-269, 2018.
• Hendi, A. “Silver nanoparticles mediate differential responses in some of liver and kidney functions during skin wound healing”, Journal of King Saud University-Science, 23, 47–52, 2011.
• Gong, C.P., Li. S.C., Wang, R.Y., “Development of biosynthesized silver nanoparticles based formulation for treating wounds during nursing care in hospitals”, Journal of Photochemistry and Photobiology B: Biology, 183, 137–141. 2018.
• Burdușel, A.C., Gherasim, O., Grumezescu, A., Mogoantă, L., Ficai, A., “Biomedical applications of silver nanoparticles: An upto-date overview”, Nanomaterials, 2018, 8(9), 681-705 2018.
• Kumar, S.S.D., Rajendran, N.K., Houreld, N.N., Abrahamse, H., “Recent advances on silver nanoparticle and biopolymer based biomaterials for wound healing applications”, International Journal of Biological Macromolecules, 115, 165-175, 2018.