Polyporus squamosus (Huds.) Fr. MANTARININ HPLC-DAD ANALİZİ, ANTİOKSİDAN, ANTİKOLİNESTERAZ VE ANTİDİYABETİK AKTİVİTELERİ

Year 2023, , 73 - 80, 31.12.2023

Gülsen Tel-çayan , Cansel Fındık

https://doi.org/10.22531/muglajsci.1336470

Abstract

Polyporus türleri, tıbbi mantarlar arasında tanımlanan biyoaktif özellikleri ile Polyporaceae familyasının değerli bir türüdür. Bu çalışma, Polyporus squamosus (Huds.) Fr. mantarının antioksidan, antikolinesteraz ve antidiyabetik aktiviteleri ile HPLC-DAD ile fenolik profilinin karakterizasyonu araştırmak için yapılmıştır. Fumarik asit (190,07±0,08 µg/g), kateşin hidrat (46,96±0,02 µg/g), ferulik asit (21,98±0,03 µg/g), trans-sinnamik asit (16,66±0,02 µg/g) ve protokateşik asit (13,29±0,06 µg/g) HPLC-DAD ile P. squamosus'ta en bol bulunan bileşikler olarak tespit edilmiştir. P. squamosus metanol ekstresi β-karoten linoleik asit (IC50: 73,75±0,28 µg/mL), DPPH• giderim (400 µg/mL’de %7,56±0,00 inhibisyon), ABTS•+ giderim (IC50: 154,30±0,55 µg/mL) ve metal kelatlama (400 µg/mL’de %35,61±1,20 inhibisyon) yöntemlerinde hekzan ekstresinden daha yüksek antioksidan aktivite göstermiştir. P. squamosus hekzan ekstresi AChE enzimine karşı 200 µg/mL’de %39,48±0,45, BChE enzimine karşı 200 µg/mL’de %28,02±1,34, α-amilaz enzimine karşı 1000 µg/mL’de %96,70±0,80 ve α-glukozidaz enzimine karşı 500 µg/mL’de %39,93±0,52 inhibisyon değerleri ile metanol ekstresinden daha güçlü bir inhibitör olarak belirlenmiştir. Mevcut çalışma, P. squamosus’un sentetik ilaçlara alternatif olarak değerlendirilebilecek değerli terapötik bileşiklerin umut verici bir kaynağı olarak önemini vurgulamıştır.

Keywords

Polyporus squamosus, Fenolik profil, HPLC-DAD, Antioksidan aktivite, Antikolinesteraz aktivite, Antidiyabetik aktivite

HPLC-DAD ANALYSIS, AND DETERMINATION OF ANTIOXIDANT, ANTICHOLINESTERASE AND ANTIDIABETIC ACTIVITIES OF Polyporus squamosus (Huds.) Fr.

Abstract

Polyporus species is a valuable species of the Polyporaceae family with defined bioactive properties among medicinal mushrooms. This study was undertaken to investigate the antioxidant, anticholinesterase and antidiabetic activities of Polyporus squamosus (Huds.) Fr. with characterization of phenolic profile by HPLC-DAD. Fumaric acid (190.07±0.08 µg/g), catechin hydrate (46.96±0.02 µg/g), ferulic acid (21.98±0.03 µg/g), trans-cinnamic acid (16.66±0.02 µg/g), and protocatechuic acid (13.29±0.06 µg/g) were detected as the most abundant compounds in P. squamosus by HPLC-DAD. P. squamosus methanol extract exhibited higher antioxidant activity than the hexane extract in β-carotene linoleic acid (IC50: 73.75±0.28 µg/mL), DPPH• scavenging (7.56±0.00% inhibition at 400 µg/mL), ABTS•+ scavenging (IC50: 154.30±0.55 µg/mL), and metal chelating (35.61±1.20% inhibition at 400 µg/mL) assays. P. squamosus hexane extract was determined as the stronger inhibitor than the methanol extract against all enzymes with inhibition values of 39.48±0.45% on AChE at 200 µg/mL, 28.02±1.34% on BChE at 200 µg/mL, 96.70±0.80% on α-amylase at 1000 µg/mL, and 39.93±0.52% on α-glucosidase at 500 µg/mL. The present study highlighted the importance of P. squamosus as a promising source of valuable therapeutic compounds that could be considered as an alternatives to synthetic drugs.

Keywords

Polyporus squamosus, Phenolic profile, HPLC-DAD, Antioxidant activity, Anticholinesterase activity, Antidiabetic activity

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