POLY(N-ISOPROPYLACRYLAMIDE) HYDROGEL INCORPORATING SQUARAINE: SYNTHESIS, DRUG DELIVERY AND PHOTODYNAMIC PROPERTIES

Abstract

In the present work, we describe the fabrication of a thermosensitive hydrogel. To fabricate the hydrogel (Sq1@PNIPAAm), we opted to use biocompatible poly(N-isopropylacrylamide) (PNIPAM) and squaraine dye (Sq1) as the polymer and the crosslinker, respectively. It is noteworthy that Sq1@PNIPAAm can be loaded with fluorescein, and we evaluated the fluorescein release behavior of Sq1@PNIPAAm hydrogel. We noted that on demand sustainable release of fluorescein was feasible upon gradual heating of Sq1@PNIPAAm hydrogel. Furthermore, Sq1@PNIPAAm hydrogels can be used as photosensitizers pertinent to photodynamic therapy (PDT). Our results show that hydrogel possesses favorable biological safety for use in in vitro anticancer studies. In vitro experiments confirmed that Sq1@PNIPAAm hydrogels could kill over 40% of cancer cells. Overall, we have successfully shown that Sq1@PNIPAAm enabled photodynamic therapy. Moreover, fluorescein loading into Sq1@PNIPAAm was possible, and it could be used to successfully accomplish temperature-controlled on-demand release. Given the abundance of low-cost, commercially accessible monomers available for use in hydrogel synthesis, this method offers access to a wide range of functional hydrogels for use in biomedical applications.

Keywords

• Poly(N-isopropylacrylamide)
• Hydrogel
• Drug release
• Photodynamic therapy
• In vitro

SKUARİN İÇEREN POLİ(N-İZOPROPİLAKRİLAMİD) HİDROJEL: SENTEZ, İLAÇ SALIMI VE FOTODİNAMİK ÖZELLİKLER

Abstract

Bu çalışmada, termo-duyarlı bir hidrojel sentezi açıklanmaktadır. Hidrojeli (Sq1@PNIPAAm) elde etmek için biyouyumlu poli(N-izopropilakrilamid) (PNIPAM) ve skuarain boyasını (Sq1) sırasıyla polimer ve çapraz bağlayıcı olarak kullanmayı tercih ettik. Sq1@PNIPAAm'ın floresin ile yüklenebileceğini ve floresin salım davranışını inceledik. Sq1@PNIPAAm hidrojelinin kademeli olarak ısıtılmasıyla istenildiğinde sürdürülebilir floresin salımının mümkün olduğunu gözlemledik. Ayrıca, Sq1@PNIPAAm hidrojelleri, fotodinamik tedavide kullanılabilecek fotoduyarlaştırıcı özellik göstermektedir. Sonuçlarımız, hidrojelin in vitro antikanser çalışmalarında kullanım için uygun biyo güvenliğe sahip olduğunu göstermektedir. In vitro deneyler, Sq1@PNIPAAm hidrojellerinin kanser hücrelerinin %40'ından fazlasını öldürebileceğini doğruladı. Genel olarak, Sq1@PNIPAAm'ın fotodinamik tedaviyi mümkün kıldığını başarıyla gösterdik. Dahası, Sq1@PNIPAAm'a ilaç yüklemek mümkündür ve sıcaklık kontrollü ilaç salımı başarıyla gerçekleştirilebilmiştir. Hidrojel sentezi için kullanılabilecek ucuz ve ticari olarak erişilebilir monomerlerin bol miktarda bulunması göz önüne alındığında, bu yöntem biyomedikal uygulamalarda kullanılmak üzere geniş bir yelpazede işlevsel hidrojellere erişim sağlar.

Keywords

• Poli(N-izopropilakrilamid)
• Hidrojel
• İlaç salımı
• Fotodinamik tedavi
• In vitro

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