REDUCED GRAPHENE OXIDE AND Tb-DO3A CONJUGATE AS LUMINESCENT CHEMOSENSOR FOR AGILE DETECTION OF HYDROXYL RADICAL

Abstract

The development of chemosensors for the detection of hydroxyl radicals (HO•) is a challenging task since HO• has an exceptionally short lifetime (in vivo half-life of 1 ns). In this work, we have designed and synthesized a versatile probe, viz. Tb@rGO, for the detection of HO• amongst the biologically important ions and reactive oxygen species (ROS). Our design is based on covalent conjugation of reduced graphene oxide (rGO) with terbium (III)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (Tb-DO3A). Tb@rGO is characterized by traditional spectroscopic methods including XRD, SEM, TEM, and zeta potential analysis. Furthermore, we elaborate the photophysical properties of Tb@rGO. Accordingly, our results attest that Tb@rGO has unique luminescence features, rendering it highly effective in the detection of HO•. Remarkably, Tb@rGO is highly selective to HO• among many biologically important species in 0.1 M pH 7.4 phosphate buffered saline solution. It is also noteworthy that the limit of detection (LOD) is 0.92 M for HO•. Therefore, this novel material hold promises as selective turn-off luminescent HO• probe.

Keywords

• Terbium (III)
• Reduced graphene oxide
• Reactive oxygen species
• Hydroxyl radical
• Luminescent probe

Supporting Institution

Aksaray University, the Scientific and Technological Research Council of Turkey, Turkish Academy of Sciences (TUBA)

Project Number

BAP 2019-19

Ethical Statement

None to declare

Thanks

Partial financial supports were provided by Aksaray University (Grant No. BAP 2019-19) and the Scientific and Technological Research Council of Turkey (TUBITAK, Grant No. 118Z293). F. A. is indebted to the Turkish Academy of Sciences (TUBA) for Outstanding Young Investigator Award (TUBA GEBIP). M.A. thanks TUBITAK for a scholarship.

HİDROKSİL RADİKALİNİN HIZLI TESPİTİ İÇİN LÜMİNESANS KEMOSENSÖR OLARAK iNDİRGENMİŞ GRAFEN OKSİT VE Tb-DO3A KONJÜGATI

Abstract

Hidroksil radikallerinin (HO•) tespiti için kemosensörlerin geliştirilmesi, HO•’nun son derece kısa bir ömre sahip olması (in vivo yarılanma ömrü ~1 ns) nedeniyle zorlu bir iştir. Bu çalışmada biyolojik olarak önemli iyonlar ve reaktif oksijen türleri (ROS) arasında HO•’nun tespiti için kullanılabilecek çok yönlü bir prob Tb@rGO tasarladık ve sentezledik. Tasarımımız indirgenmiş grafen oksidin (rGO) terbiyum (III)-1,4,7,10-tetraazasiklododekan-1,4,7-triasetik asit (Tb-DO3A) ile kovalent konjugasyonuna dayanmaktadır. Tb@rGO, XRD, SEM, TEM ve zeta potansiyel analizini içeren geleneksel spektroskopik yöntemlerle karakterize edilmiştir. Ayrıca Tb@rGO’nun fotofiziksel özelliklerini de detaylandırdık. Buna göre, sonuçlarımız Tb@rGO’nun benzersiz lüminesans özelliklere sahip olduğunu ve bu özelliğin onu HO• tespitinde son derece etkili kıldığını doğrulamaktadır. Dikkat çekici bir şekilde Tb@rGO fosfat tamponlu salin (0,1 M PBS, pH 7,4) çözeltisinde biyolojik açıdan önemli birçok tür arasında HO•’ya karşı oldukça seçicidir. HO• için tespit sınırının (LOD) 0,92 M olması da dikkat çekicidir. Bu nedenle, bu yeni malzeme, seçici lüminesans sönümlemeli HO• probu olarak umut vaat etmektedir.

Keywords

• Terbiyum (III)
• İndirgenmiş grafen oksit
• Reaktif oksijen türleri
• Hidroksil radikali
• Lüminesans prob

Project Number

BAP 2019-19

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