RELİKT ENDEMİK LIQUIDAMBAR ORIENTALIS (Miller )’İN HİPOKOTİL VE KOTİLEDON EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU

Abstract

Bu çalışmada, Bitki Büyüme Düzenleyicileri (BBD)’nin [ 6-Benzil Amino Pürin (BAP) (0, 1.0, 2.0, 3.0 mg L^-1) tek başına ve BAP’ın Naftalen Asetik Asit (NAA) (0, 0.05, 0.10, 0.15 mg L^-1) veya İndol Bütirik Asit (IBA) (0, 0.01, 0.05, 0.10 mg L^-1)ile kombinasyonları] Liquidambar orientalis (Anadolu sığlası)’in 4-5 haftalık steril fidelerinin hipokotil ve kotiledon eksplantlarından direkt organogenez üzerine etkisi araştırılmıştır. Organogenez için ortama BAP ilavesi gerekli bulunmuş ayrıca, BAP’ın NAA ile kombinasyonları IBA ile kombinasyonlarına göre, gerek sürgün oluşturan eksplant yüzdesi gerekse eksplant başına sürgün sayısı ve sürgünlerin gelişimi bakımından daha iyi sonuç vermiştir. Ortalama en yüksek sürgün oluşturan eksplant yüzdesi (%76.64), 0.10 mg L^-1NAA + 3.0 mg L^-1BAP içeren Woody Plant Medium (WPM)’da kültüre alınan hipokotil eksplantlarından elde edilmiştir. Eksplant başına ortalama en yüksek sürgün sayısı (32.20 sürgün/eksplant), 0.10 mg L^-1IBA + 1.0 mg L^-1BAP eklenmiş WPM besin ortamında kültüre alınan hipokotil eksplantlarında gözlenmiştir. Her iki eksplant için de 0.05 mg L^-1NAA’in 1.0 veya 2.0 mg L^-1BAP ile kombinasyonu uygun bulunmuştur.

Keywords

• Direkt organogenez,Liquidambar orientalis,Anadolu sığlası,Bitki Büyüme Düzenleyicileri (BBD)

ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller

Abstract

In this study, the effect of Plant Growth Regulators (PGRs) [6-Benzylaminopurine (BAP)(0, 1.0, 2.0, 3.0 mg L^-1) alone and BAP in combinations with α-Naphthaleneacetic acid (NAA)(0, 0.05, 0.10, 0.15 mg L^-1) or Indole-3-butyric acid (IBA)(0, 0.01, 0.05, 0.10 mg L^-1)] on direct organogenesis from hypocotyl and cotyledon explants of 4-5-week-old sterile seedlings of Liquidambar orientalis (Oriental sweetgum) were investigated. For organogenesis, the addition of BAP to the medium was required, also the combinations of BAP with NAA responded better in terms of the percentage of shoot-forming explants, the number of shoots per explant and the growth of shoots as compared to combinations of BAP with IBA. The highest average percentage of shoot formation (76.64%) was obtained from hypocotyl explants cultured on Woody Plant Medium (WPM) containing 0.10 mg L^-1NAA + 3.0 mg L^-1BAP. The highest average shoot number per explant (32.20 shoots/explant) was observed in also hypocotyl explants cultured on WPM supplemented with 0.10 mg L^-1IBA + 1.00 mg L^-1BAP. The combination of 0.05 mg L^-1NAA + 1.0 or 2.0 mg L^-1BAP was found to be appropriate for both explants.

Keywords

• Direct organogenesis,Liquidambar orientalis,Oriental sweetgum,Plant Growth Regulators (PGRs)

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