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INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE

Year 2019, , 17 - 23, 30.06.2019
https://doi.org/10.22531/muglajsci.495209

Abstract

Since carbon nanotubes and conjugated polymers promise to lead a number of real-world technologies, herein, an electrochemical biosensor, using poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1’,3}-thiadiazole)] (PFBTz) and multi-walled carbon nanotubes (MWCNTs) was constructed for glucose sensing. To create such a surface, a graphite electrode was covered with PFBTz and MWCNTs, respectively. Then, glucose oxidase (GOx) was immobilized onto this new platform using glutaraldehyde (GA) as the cross linker. The limit of detection, linear range and sensitivity values were found to be 0.018 mM, 0.025–0.6 mM and 66.0 μAmM-1cm-2, respectively. The selectivity of the prepared biosensor was also examined in the presence of interferences and as a result, it has been observed that the biosensor response was much higher for glucose in all measurements. Surface characterizations before and after enzyme immobilization were done using scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. In the final part of the study, the constructed biosensor was applied to beverages for glucose detection and very promising results were obtained. With this work, a robust, novel and economic sensing platform was prepared for glucose determination.

References

  • [1] Harper, A. and Anderson, M.R. “Electrochemical glucose sensors—developments using electrostatic assembly and carbon nanotubes for biosensor construction”, Sensors, 10, 8248–8274, 2010.[2] Wang, J. “Electrochemical Glucose Biosensors”, Chem. Rev., 108, 814-825, 2008.[3] Singh, S., Chaubey, A., Malhotra, B.D. “Amperometric cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on conducting polypyrrole films”, Anal. Chim. Acta, 502, 229-234, 2004.[4] Cesarino, I., Moraes, F.C., Lanza, M.R.V., Machado, S.A. “Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline–carbon nanotubes”, Food Chem., 135, 873-879, 2012.[5] Soylemez, S., Yilmaz, T., Buber, E., Udum, Y.A., Ozcubukcu, S., Toppare, L. “Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates”, J.Mat.Chem. B., 5, 7384-7392, 2017.[6] Bicak, T.C., Gicevičius, M., Gokoglan, T.C., Yilmaz, G., Ramanavicius, A., Toppare, L., Yagci, Y. “Simultaneous and sequential synthesis of Polyaniline‑g‑poly(ethylene glycol) by combination of oxidative polymerization and CuAAC click chemistry: A water-soluble”, Macromolecules, 50, 1824−1831, 2017.[7] Malhotra, B. D. and Chaubey, A. “Biosensors for clinical diagnostics industry”, Sensor. Actuat. B-Chem., 91, 117-127, 2003.[8]Mazeiko, V., Kausaite-Minkstimiene, A., Ramanaviciene, A., Balevicius, Z., Ramanavicius, A. “Gold nanoparticle and conducting polymer-polyaniline-based nanocomposites for glucose biosensor design”, Sens. Actuat. B-Chem., 189, 187-193, 2013.[9]Siriviriyanun, A., Imae, T., Nagatani, N. “Electrochemical biosensors for biocontaminant detection consisting of carbon nanotubes, platinum nanoparticles, dendrimers, and enzymes”, Anal. Biochem., 443, 169–171, 2013.[10] Yoo, E., Lee, S. “Glucose biosensors: an overview of use in clinical practice”, Sensors, 10, 4558-4576, 2010.[11]Bankar, S.B., Bule, M.V., Singhal, R.S., Ananthanarayan, L. “Glucose oxidase — An overview”, Biotechnol. Adv., 27, 489-501, 2009.[12] Leskovac V., Trivić, S., Wohlfahrt, G., Kandrac, J., Pericin, D. “Glucose oxidase from Aspergillus niger: the mechanism of action with molecular oxygen, quinones, and one-electron acceptors”, Int. J. Biochem. Cell Biol., 37, 731-750, 2005.[13] Brights, H.J., and Appleby, M. “The pH Dependence of the Individual Steps in the Glucose Oxidase Reaction”, J. Biol. Chem., 244, 3625-3634, 1969.[14] Bard, A.J., and Faulkner, L.R. “Electrochemical methods: Fundamentals and applications”, New York: John Wiley, 2000.[15] Liu, Y., Wang, M., Zhao, F., Xu, Z., Dong, S. “The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix”, Biosens. Bioelectron. 21, 984–988, 2005.[16] Ayranci, R., Demirkol, D.O., Ak, M., Timur, S. “Ferrocene-functionalized 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline: A novel design in conducting polymer-based electrochemical biosensors”, Sensors, 15, 1389-1403, 2015.[17] Deng, S., Jian, G., Lei, J., Hu, Z., Ju, H. “A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes”, Biosens. Bioelectron., 25, 373-377, 2009.[18] Chen, Y., Li, Y., Sun, D., Tian, D., Zhang, J., Zhu, J.J. “Fabrication of gold nanoparticles on bilayer graphene for glucose electrochemical biosensing”, J. Mater. Chem., 21, 7604-7611, 2011.[19] Vukojević, V., Djurdjić, S., Ognjanović, M., Fabián, M., Samphao, A., Kalcher, K., Stanković, D.M. “Enzymatic glucose biosensor based on manganese dioxide nanoparticles decorated on graphene nanoribbons”, J. Electroanal. Chem. 823, 610–616, 2018.[20]Palanisamy, S., Cheemalapati, S., Chen, S.M. “Amperometric glucose biosensor based on glucose oxidase dispersed in multiwalled carbon nanotubes/graphene oxide hybrid biocomposite”, Mater. Sci. Eng. C, 34, 207–213, 2014.[21] Ekabutr, P., Chailapakul, O., Supaphol, P. “Modification of Disposable Screen-Printed Carbon Electrode Surfaces with Conductive Electrospun Nanofibers for Biosensor Applications”, J. Appl. Polym. Sci., 130, 3885-3893, 2013.
Year 2019, , 17 - 23, 30.06.2019
https://doi.org/10.22531/muglajsci.495209

Abstract

References

  • [1] Harper, A. and Anderson, M.R. “Electrochemical glucose sensors—developments using electrostatic assembly and carbon nanotubes for biosensor construction”, Sensors, 10, 8248–8274, 2010.[2] Wang, J. “Electrochemical Glucose Biosensors”, Chem. Rev., 108, 814-825, 2008.[3] Singh, S., Chaubey, A., Malhotra, B.D. “Amperometric cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on conducting polypyrrole films”, Anal. Chim. Acta, 502, 229-234, 2004.[4] Cesarino, I., Moraes, F.C., Lanza, M.R.V., Machado, S.A. “Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline–carbon nanotubes”, Food Chem., 135, 873-879, 2012.[5] Soylemez, S., Yilmaz, T., Buber, E., Udum, Y.A., Ozcubukcu, S., Toppare, L. “Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates”, J.Mat.Chem. B., 5, 7384-7392, 2017.[6] Bicak, T.C., Gicevičius, M., Gokoglan, T.C., Yilmaz, G., Ramanavicius, A., Toppare, L., Yagci, Y. “Simultaneous and sequential synthesis of Polyaniline‑g‑poly(ethylene glycol) by combination of oxidative polymerization and CuAAC click chemistry: A water-soluble”, Macromolecules, 50, 1824−1831, 2017.[7] Malhotra, B. D. and Chaubey, A. “Biosensors for clinical diagnostics industry”, Sensor. Actuat. B-Chem., 91, 117-127, 2003.[8]Mazeiko, V., Kausaite-Minkstimiene, A., Ramanaviciene, A., Balevicius, Z., Ramanavicius, A. “Gold nanoparticle and conducting polymer-polyaniline-based nanocomposites for glucose biosensor design”, Sens. Actuat. B-Chem., 189, 187-193, 2013.[9]Siriviriyanun, A., Imae, T., Nagatani, N. “Electrochemical biosensors for biocontaminant detection consisting of carbon nanotubes, platinum nanoparticles, dendrimers, and enzymes”, Anal. Biochem., 443, 169–171, 2013.[10] Yoo, E., Lee, S. “Glucose biosensors: an overview of use in clinical practice”, Sensors, 10, 4558-4576, 2010.[11]Bankar, S.B., Bule, M.V., Singhal, R.S., Ananthanarayan, L. “Glucose oxidase — An overview”, Biotechnol. Adv., 27, 489-501, 2009.[12] Leskovac V., Trivić, S., Wohlfahrt, G., Kandrac, J., Pericin, D. “Glucose oxidase from Aspergillus niger: the mechanism of action with molecular oxygen, quinones, and one-electron acceptors”, Int. J. Biochem. Cell Biol., 37, 731-750, 2005.[13] Brights, H.J., and Appleby, M. “The pH Dependence of the Individual Steps in the Glucose Oxidase Reaction”, J. Biol. Chem., 244, 3625-3634, 1969.[14] Bard, A.J., and Faulkner, L.R. “Electrochemical methods: Fundamentals and applications”, New York: John Wiley, 2000.[15] Liu, Y., Wang, M., Zhao, F., Xu, Z., Dong, S. “The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix”, Biosens. Bioelectron. 21, 984–988, 2005.[16] Ayranci, R., Demirkol, D.O., Ak, M., Timur, S. “Ferrocene-functionalized 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline: A novel design in conducting polymer-based electrochemical biosensors”, Sensors, 15, 1389-1403, 2015.[17] Deng, S., Jian, G., Lei, J., Hu, Z., Ju, H. “A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes”, Biosens. Bioelectron., 25, 373-377, 2009.[18] Chen, Y., Li, Y., Sun, D., Tian, D., Zhang, J., Zhu, J.J. “Fabrication of gold nanoparticles on bilayer graphene for glucose electrochemical biosensing”, J. Mater. Chem., 21, 7604-7611, 2011.[19] Vukojević, V., Djurdjić, S., Ognjanović, M., Fabián, M., Samphao, A., Kalcher, K., Stanković, D.M. “Enzymatic glucose biosensor based on manganese dioxide nanoparticles decorated on graphene nanoribbons”, J. Electroanal. Chem. 823, 610–616, 2018.[20]Palanisamy, S., Cheemalapati, S., Chen, S.M. “Amperometric glucose biosensor based on glucose oxidase dispersed in multiwalled carbon nanotubes/graphene oxide hybrid biocomposite”, Mater. Sci. Eng. C, 34, 207–213, 2014.[21] Ekabutr, P., Chailapakul, O., Supaphol, P. “Modification of Disposable Screen-Printed Carbon Electrode Surfaces with Conductive Electrospun Nanofibers for Biosensor Applications”, J. Appl. Polym. Sci., 130, 3885-3893, 2013.
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Details

Primary Language English
Subjects Engineering
Journal Section Journals
Authors

Saniye Söylemez 0000-0002-8955-133X

Publication Date June 30, 2019
Published in Issue Year 2019

Cite

APA Söylemez, S. (2019). INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE. Mugla Journal of Science and Technology, 5(1), 17-23. https://doi.org/10.22531/muglajsci.495209
AMA Söylemez S. INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE. MJST. June 2019;5(1):17-23. doi:10.22531/muglajsci.495209
Chicago Söylemez, Saniye. “INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-Co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE”. Mugla Journal of Science and Technology 5, no. 1 (June 2019): 17-23. https://doi.org/10.22531/muglajsci.495209.
EndNote Söylemez S (June 1, 2019) INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE. Mugla Journal of Science and Technology 5 1 17–23.
IEEE S. Söylemez, “INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE”, MJST, vol. 5, no. 1, pp. 17–23, 2019, doi: 10.22531/muglajsci.495209.
ISNAD Söylemez, Saniye. “INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-Co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE”. Mugla Journal of Science and Technology 5/1 (June 2019), 17-23. https://doi.org/10.22531/muglajsci.495209.
JAMA Söylemez S. INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE. MJST. 2019;5:17–23.
MLA Söylemez, Saniye. “INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-Co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE”. Mugla Journal of Science and Technology, vol. 5, no. 1, 2019, pp. 17-23, doi:10.22531/muglajsci.495209.
Vancouver Söylemez S. INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE. MJST. 2019;5(1):17-23.

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